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Erdosteine ameliorates neurological outcome and oxidative stress due to ischemia/reperfusion injury in rabbit spinal cord.
Eur J Vasc Endovasc Surg. 2004 Oct; 28(4):379-86.EJ

Abstract

OBJECTIVE

Oxygen-derived free radicals have been suggested as important in degeneration after spinal cord ischemia. The aim of this study was to investigate whether erdosteine has a protective effect against spinal cord ischemia during aortic cross clamping.

MATERIALS AND METHODS

New Zealand White rabbits (n=21) were divided into three groups. In the ischemia/reperfusion group (I/R) (n=8), the infrarenal aorta of rabbits was cross clamped for 21 min and then reperfused. In erdosteine group, the administration of erdosteine solution (50 mg/kg) was started two days before aortic cross-clamping and rabbits (n=8) were subjected to ischemia and reperfusion. Animals in control group (n=5) underwent a surgical procedure similar to the other groups but the aorta was not clamped. The animals were sacrificed at 72 h and histopathological, and biochemical analyses were carried out on the lumbar spinal cords.

RESULTS

Erdosteine treatment was associated with improved neurological function in the postoperative period. Histopathological examination of spinal cord tissues in erdosteine group revealed changes consistent with mild ischemic injury, but rabbits in I/R group with paraplegia had total destruction of the motor neurons. Biochemical analyses of spinal cord tissues, in the I/R group, revealed a significant increase in the superoxide dismutase, xanthine oxidase, adenosine deaminase and myeloperoxidase activities, and a significant depletion in glutathione peroxidase activity when compared to that of control rabbits. Erdosteine treatment prevented the increase of all these enzymes except adenosine deaminase. Ischemia/reperfusion produced a significant increase in the tissue malondialdehyde levels. Ischemia/reperfusion-induced increments in malondialdehyde content of the spinal cord were significantly prevented by erdosteine treatment.

CONCLUSIONS

The present study demonstrated that erdosteine treatment before aortic cross clamping ameliorates neurological outcome, neuronal injury and oxidative stress in the rabbit spinal cord.

Authors+Show Affiliations

Department of Cardiovascular Surgery, Turgut Ozal Medical Center, Inonu University, Malatya, Turkey.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Evaluation Study
Journal Article

Language

eng

PubMed ID

15350559

Citation

Ege, E, et al. "Erdosteine Ameliorates Neurological Outcome and Oxidative Stress Due to Ischemia/reperfusion Injury in Rabbit Spinal Cord." European Journal of Vascular and Endovascular Surgery : the Official Journal of the European Society for Vascular Surgery, vol. 28, no. 4, 2004, pp. 379-86.
Ege E, Ilhan A, Gurel A, et al. Erdosteine ameliorates neurological outcome and oxidative stress due to ischemia/reperfusion injury in rabbit spinal cord. Eur J Vasc Endovasc Surg. 2004;28(4):379-86.
Ege, E., Ilhan, A., Gurel, A., Akyol, O., & Ozen, S. (2004). Erdosteine ameliorates neurological outcome and oxidative stress due to ischemia/reperfusion injury in rabbit spinal cord. European Journal of Vascular and Endovascular Surgery : the Official Journal of the European Society for Vascular Surgery, 28(4), 379-86.
Ege E, et al. Erdosteine Ameliorates Neurological Outcome and Oxidative Stress Due to Ischemia/reperfusion Injury in Rabbit Spinal Cord. Eur J Vasc Endovasc Surg. 2004;28(4):379-86. PubMed PMID: 15350559.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Erdosteine ameliorates neurological outcome and oxidative stress due to ischemia/reperfusion injury in rabbit spinal cord. AU - Ege,E, AU - Ilhan,A, AU - Gurel,A, AU - Akyol,O, AU - Ozen,S, PY - 2004/06/03/accepted PY - 2004/9/8/pubmed PY - 2004/11/9/medline PY - 2004/9/8/entrez SP - 379 EP - 86 JF - European journal of vascular and endovascular surgery : the official journal of the European Society for Vascular Surgery JO - Eur J Vasc Endovasc Surg VL - 28 IS - 4 N2 - OBJECTIVE: Oxygen-derived free radicals have been suggested as important in degeneration after spinal cord ischemia. The aim of this study was to investigate whether erdosteine has a protective effect against spinal cord ischemia during aortic cross clamping. MATERIALS AND METHODS: New Zealand White rabbits (n=21) were divided into three groups. In the ischemia/reperfusion group (I/R) (n=8), the infrarenal aorta of rabbits was cross clamped for 21 min and then reperfused. In erdosteine group, the administration of erdosteine solution (50 mg/kg) was started two days before aortic cross-clamping and rabbits (n=8) were subjected to ischemia and reperfusion. Animals in control group (n=5) underwent a surgical procedure similar to the other groups but the aorta was not clamped. The animals were sacrificed at 72 h and histopathological, and biochemical analyses were carried out on the lumbar spinal cords. RESULTS: Erdosteine treatment was associated with improved neurological function in the postoperative period. Histopathological examination of spinal cord tissues in erdosteine group revealed changes consistent with mild ischemic injury, but rabbits in I/R group with paraplegia had total destruction of the motor neurons. Biochemical analyses of spinal cord tissues, in the I/R group, revealed a significant increase in the superoxide dismutase, xanthine oxidase, adenosine deaminase and myeloperoxidase activities, and a significant depletion in glutathione peroxidase activity when compared to that of control rabbits. Erdosteine treatment prevented the increase of all these enzymes except adenosine deaminase. Ischemia/reperfusion produced a significant increase in the tissue malondialdehyde levels. Ischemia/reperfusion-induced increments in malondialdehyde content of the spinal cord were significantly prevented by erdosteine treatment. CONCLUSIONS: The present study demonstrated that erdosteine treatment before aortic cross clamping ameliorates neurological outcome, neuronal injury and oxidative stress in the rabbit spinal cord. SN - 1078-5884 UR - https://www.unboundmedicine.com/medline/citation/15350559/Erdosteine_ameliorates_neurological_outcome_and_oxidative_stress_due_to_ischemia/reperfusion_injury_in_rabbit_spinal_cord_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1078588404002618 DB - PRIME DP - Unbound Medicine ER -