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HPTLC method for guggulsterone. I. Quantitative determination of E- and Z-guggulsterone in herbal extract and pharmaceutical dosage form.
J Pharm Biomed Anal. 2004 Sep 21; 36(1):33-41.JP

Abstract

A sensitive, selective, precise and robust high-performance thin-layer chromatographic method of analysis of E and Z stereoisomers of guggulsterone (the hypolipidemic agent in the gum-resin exudates of Commiphora mukul) both as a bulk drug and in formulations was developed and validated. The method employed TLC aluminium plates precoated with silica gel 60F-254 as the stationary phase. The solvent system consisted of toluene-acetone (9:1, v/v). Densitometric analysis of guggulsterone was carried out in the absorbance mode at 250 nm. This system was found to give compact spots for E- and Z-guggulsterone (Rf value of 0.38 +/- 0.02 and 0.46 +/- 0.02, respectively) following double development of chromatoplates with the same mobile phase. The linear regression analysis data for the calibration plots for E- and Z-guggulsterone showed good linear relationship with r2 = 0.9977 +/- 0.054 and 0.9975 +/- 0.068, respectively, in the concentration range of 100-6000 ng/spot. The mean value of slope and intercept were 0.11 +/- 0.006 and 0.11 +/- 0.005, 14.26 +/- 0.56 and 10.92 +/- 0.76, respectively, for E- and Z-guggulsterone. The method was validated for precision, robustness and recovery. The limit of detection and quantitation were 12, 10 and 24, 20 ng/spot, respectively, for E- and Z-guggulsterone. Statistical analysis proves that the method is repeatable and selective for the estimation of the said drug. Since the proposed mobile phase effectively resolves the E- and Z-isomers of guggulsterone, this HPTLC method can be applied for identification and quantitation of these isomers in herbal extracts and pharmaceutical dosage form.

Authors+Show Affiliations

Agrawal H
Department of Quality Assurance Techniques, Poona College of Pharmacy, Bharati Vidyapeeth Deemed University, Erandwane, Pune 411038, Maharashtra, India.
Kaul N
No affiliation info available
Paradkar AR
No affiliation info available
Mahadik KR
No affiliation info available

MeSH

CalibrationCapsulesChromatography, High Pressure LiquidChromatography, Thin LayerCommiphoraLinear ModelsPlant ExtractsPregnenedionesReference StandardsReproducibility of ResultsSensitivity and SpecificityStereoisomerism

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Validation Study

Language

eng

PubMed ID

15351045

Citation

Agrawal, Himani, et al. "HPTLC Method for Guggulsterone. I. Quantitative Determination of E- and Z-guggulsterone in Herbal Extract and Pharmaceutical Dosage Form." Journal of Pharmaceutical and Biomedical Analysis, vol. 36, no. 1, 2004, pp. 33-41.
Agrawal H, Kaul N, Paradkar AR, et al. HPTLC method for guggulsterone. I. Quantitative determination of E- and Z-guggulsterone in herbal extract and pharmaceutical dosage form. J Pharm Biomed Anal. 2004;36(1):33-41.
Agrawal, H., Kaul, N., Paradkar, A. R., & Mahadik, K. R. (2004). HPTLC method for guggulsterone. I. Quantitative determination of E- and Z-guggulsterone in herbal extract and pharmaceutical dosage form. Journal of Pharmaceutical and Biomedical Analysis, 36(1), 33-41.
Agrawal H, et al. HPTLC Method for Guggulsterone. I. Quantitative Determination of E- and Z-guggulsterone in Herbal Extract and Pharmaceutical Dosage Form. J Pharm Biomed Anal. 2004 Sep 21;36(1):33-41. PubMed PMID: 15351045.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - HPTLC method for guggulsterone. I. Quantitative determination of E- and Z-guggulsterone in herbal extract and pharmaceutical dosage form. AU - Agrawal,Himani, AU - Kaul,Neeraj, AU - Paradkar,A R, AU - Mahadik,K R, PY - 2003/11/25/received PY - 2004/04/20/revised PY - 2004/04/20/accepted PY - 2004/9/8/pubmed PY - 2005/2/11/medline PY - 2004/9/8/entrez SP - 33 EP - 41 JF - Journal of pharmaceutical and biomedical analysis JO - J Pharm Biomed Anal VL - 36 IS - 1 N2 - A sensitive, selective, precise and robust high-performance thin-layer chromatographic method of analysis of E and Z stereoisomers of guggulsterone (the hypolipidemic agent in the gum-resin exudates of Commiphora mukul) both as a bulk drug and in formulations was developed and validated. The method employed TLC aluminium plates precoated with silica gel 60F-254 as the stationary phase. The solvent system consisted of toluene-acetone (9:1, v/v). Densitometric analysis of guggulsterone was carried out in the absorbance mode at 250 nm. This system was found to give compact spots for E- and Z-guggulsterone (Rf value of 0.38 +/- 0.02 and 0.46 +/- 0.02, respectively) following double development of chromatoplates with the same mobile phase. The linear regression analysis data for the calibration plots for E- and Z-guggulsterone showed good linear relationship with r2 = 0.9977 +/- 0.054 and 0.9975 +/- 0.068, respectively, in the concentration range of 100-6000 ng/spot. The mean value of slope and intercept were 0.11 +/- 0.006 and 0.11 +/- 0.005, 14.26 +/- 0.56 and 10.92 +/- 0.76, respectively, for E- and Z-guggulsterone. The method was validated for precision, robustness and recovery. The limit of detection and quantitation were 12, 10 and 24, 20 ng/spot, respectively, for E- and Z-guggulsterone. Statistical analysis proves that the method is repeatable and selective for the estimation of the said drug. Since the proposed mobile phase effectively resolves the E- and Z-isomers of guggulsterone, this HPTLC method can be applied for identification and quantitation of these isomers in herbal extracts and pharmaceutical dosage form. SN - 0731-7085 UR - https://www.unboundmedicine.com/medline/citation/15351045/HPTLC_method_for_guggulsterone__I__Quantitative_determination_of_E__and_Z_guggulsterone_in_herbal_extract_and_pharmaceutical_dosage_form_ DB - PRIME DP - Unbound Medicine ER -