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Rapid analysis of docetaxel in human plasma by tandem mass spectrometry with on-line sample extraction.
J Pharm Biomed Anal. 2004 Sep 21; 36(1):125-31.JP

Abstract

A simple, rapid, and sensitive analytical method for the measurement of docetaxel in human plasma was developed and validated. The method is based on positive electrospray ionization tandem mass spectrometry (ESI+-MS-MS) with on-line sample extraction. It uses paclitaxel as internal standard for calibration. The on-line sample extraction minimizes sample handling and is readily adopted for automation. Quantitation of plasma docetaxel was done by the multiple reaction monitoring (MRM) mode. The method had a linear calibration range of 1.00-3000 ng/mL with a correlation coefficient >0.9999. The limit of quantitation (LOQ) for docetaxel in plasma was 1.00 ng/mL. The on-line extraction recovery of docetaxel was between 86.1-94.7%, with %CV < or = 6.1%. This method has high accuracy (90.1-96.3%), and excellent intra-assay (0.6-3.8%) and inter-assay (2.0-5.7%) precision. Its applicability to clinical samples was demonstrated by measuring patient plasma samples after treatment of weekly docetaxel at 25 mg/m2 as 60-min infusion.

Authors+Show Affiliations

Grozav AG
Department of Chemistry, Cleveland State University, 2399 Euclid Avenue, Cleveland, OH 44115, USA.
Hutson TE
No affiliation info available
Zhou X
No affiliation info available
Bukowski RM
No affiliation info available
Ganapathi R
No affiliation info available
Xu Y
No affiliation info available

MeSH

CalibrationDocetaxelHumansReproducibility of ResultsSensitivity and SpecificitySpecimen HandlingSpectrometry, Mass, Electrospray IonizationTaxoidsTime Factors

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Validation Study

Language

eng

PubMed ID

15351056

Citation

Grozav, Adrian G., et al. "Rapid Analysis of Docetaxel in Human Plasma By Tandem Mass Spectrometry With On-line Sample Extraction." Journal of Pharmaceutical and Biomedical Analysis, vol. 36, no. 1, 2004, pp. 125-31.
Grozav AG, Hutson TE, Zhou X, et al. Rapid analysis of docetaxel in human plasma by tandem mass spectrometry with on-line sample extraction. J Pharm Biomed Anal. 2004;36(1):125-31.
Grozav, A. G., Hutson, T. E., Zhou, X., Bukowski, R. M., Ganapathi, R., & Xu, Y. (2004). Rapid analysis of docetaxel in human plasma by tandem mass spectrometry with on-line sample extraction. Journal of Pharmaceutical and Biomedical Analysis, 36(1), 125-31.
Grozav AG, et al. Rapid Analysis of Docetaxel in Human Plasma By Tandem Mass Spectrometry With On-line Sample Extraction. J Pharm Biomed Anal. 2004 Sep 21;36(1):125-31. PubMed PMID: 15351056.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rapid analysis of docetaxel in human plasma by tandem mass spectrometry with on-line sample extraction. AU - Grozav,Adrian G, AU - Hutson,Thomas E, AU - Zhou,Xiang, AU - Bukowski,Ronald M, AU - Ganapathi,Ram, AU - Xu,Yan, PY - 2004/03/20/received PY - 2004/05/09/revised PY - 2004/05/14/accepted PY - 2004/9/8/pubmed PY - 2005/2/11/medline PY - 2004/9/8/entrez SP - 125 EP - 31 JF - Journal of pharmaceutical and biomedical analysis JO - J Pharm Biomed Anal VL - 36 IS - 1 N2 - A simple, rapid, and sensitive analytical method for the measurement of docetaxel in human plasma was developed and validated. The method is based on positive electrospray ionization tandem mass spectrometry (ESI+-MS-MS) with on-line sample extraction. It uses paclitaxel as internal standard for calibration. The on-line sample extraction minimizes sample handling and is readily adopted for automation. Quantitation of plasma docetaxel was done by the multiple reaction monitoring (MRM) mode. The method had a linear calibration range of 1.00-3000 ng/mL with a correlation coefficient >0.9999. The limit of quantitation (LOQ) for docetaxel in plasma was 1.00 ng/mL. The on-line extraction recovery of docetaxel was between 86.1-94.7%, with %CV < or = 6.1%. This method has high accuracy (90.1-96.3%), and excellent intra-assay (0.6-3.8%) and inter-assay (2.0-5.7%) precision. Its applicability to clinical samples was demonstrated by measuring patient plasma samples after treatment of weekly docetaxel at 25 mg/m2 as 60-min infusion. SN - 0731-7085 UR - https://www.unboundmedicine.com/medline/citation/15351056/Rapid_analysis_of_docetaxel_in_human_plasma_by_tandem_mass_spectrometry_with_on_line_sample_extraction_ DB - PRIME DP - Unbound Medicine ER -