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Adsorptive stripping voltammetric quantification of the antipsychotic drug clozapine in bulk form, pharmaceutical formulation and human serum at a mercury electrode.
J Pharm Biomed Anal. 2004 Sep 21; 36(1):149-56.JP

Abstract

Using the cyclic voltammetry technique and the Britton-Robinson buffer (pH 2-10) as a supporting electrolyte, clozapine was found to reduce at the hanging mercury drop electrode in a single two-electron irreversible step corresponding to reduction of the azomethine group of the seven-member heterocyclic ring. Based on the interfacial adsorptive character of clozapine onto the hanging mercury drop electrode, a validated square-wave adsorptive cathodic stripping (SWAdCS) voltammetric procedure was described for the quantification of bulk clozapine with limits of detection and quantitation of 4.5 x 10(-10) and 1.5 x 10(-9) M, respectively. The proposed procedure was successfully applied to the quantification of the drug in pharmaceutical formulation (Leponex) and human serum without the necessity for samples' pretreatment or any time-consuming extraction or evaporation steps prior to the analysis. The limits of detection and quantitation of clozapine in spiked human serum were found to be 1 x 10(-9) and 3.3 x 10(-9) M, respectively. The proposed procedure for quantification of clozapine in bulk form, tablets and human serum has the advantage of being simple, rapid, sensitive, precise and inexpensive compared to most of the reported methods.

Authors+Show Affiliations

Chemistry Department, Faculty of Science, Tanta University, 31527 Tanta, Egypt. essamhammam2k@hotmail.comNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15351059

Citation

Hammam, E, et al. "Adsorptive Stripping Voltammetric Quantification of the Antipsychotic Drug Clozapine in Bulk Form, Pharmaceutical Formulation and Human Serum at a Mercury Electrode." Journal of Pharmaceutical and Biomedical Analysis, vol. 36, no. 1, 2004, pp. 149-56.
Hammam E, Tawfik A, Ghoneim MM. Adsorptive stripping voltammetric quantification of the antipsychotic drug clozapine in bulk form, pharmaceutical formulation and human serum at a mercury electrode. J Pharm Biomed Anal. 2004;36(1):149-56.
Hammam, E., Tawfik, A., & Ghoneim, M. M. (2004). Adsorptive stripping voltammetric quantification of the antipsychotic drug clozapine in bulk form, pharmaceutical formulation and human serum at a mercury electrode. Journal of Pharmaceutical and Biomedical Analysis, 36(1), 149-56.
Hammam E, Tawfik A, Ghoneim MM. Adsorptive Stripping Voltammetric Quantification of the Antipsychotic Drug Clozapine in Bulk Form, Pharmaceutical Formulation and Human Serum at a Mercury Electrode. J Pharm Biomed Anal. 2004 Sep 21;36(1):149-56. PubMed PMID: 15351059.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Adsorptive stripping voltammetric quantification of the antipsychotic drug clozapine in bulk form, pharmaceutical formulation and human serum at a mercury electrode. AU - Hammam,E, AU - Tawfik,A, AU - Ghoneim,M M, PY - 2004/01/22/received PY - 2004/04/20/revised PY - 2004/04/25/accepted PY - 2004/9/8/pubmed PY - 2005/2/11/medline PY - 2004/9/8/entrez SP - 149 EP - 56 JF - Journal of pharmaceutical and biomedical analysis JO - J Pharm Biomed Anal VL - 36 IS - 1 N2 - Using the cyclic voltammetry technique and the Britton-Robinson buffer (pH 2-10) as a supporting electrolyte, clozapine was found to reduce at the hanging mercury drop electrode in a single two-electron irreversible step corresponding to reduction of the azomethine group of the seven-member heterocyclic ring. Based on the interfacial adsorptive character of clozapine onto the hanging mercury drop electrode, a validated square-wave adsorptive cathodic stripping (SWAdCS) voltammetric procedure was described for the quantification of bulk clozapine with limits of detection and quantitation of 4.5 x 10(-10) and 1.5 x 10(-9) M, respectively. The proposed procedure was successfully applied to the quantification of the drug in pharmaceutical formulation (Leponex) and human serum without the necessity for samples' pretreatment or any time-consuming extraction or evaporation steps prior to the analysis. The limits of detection and quantitation of clozapine in spiked human serum were found to be 1 x 10(-9) and 3.3 x 10(-9) M, respectively. The proposed procedure for quantification of clozapine in bulk form, tablets and human serum has the advantage of being simple, rapid, sensitive, precise and inexpensive compared to most of the reported methods. SN - 0731-7085 UR - https://www.unboundmedicine.com/medline/citation/15351059/Adsorptive_stripping_voltammetric_quantification_of_the_antipsychotic_drug_clozapine_in_bulk_form_pharmaceutical_formulation_and_human_serum_at_a_mercury_electrode_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0731708504002067 DB - PRIME DP - Unbound Medicine ER -