Tags

Type your tag names separated by a space and hit enter

Determination of nateglinide in animal plasma by micellar electrokinetic chromatography and on-line sweeping technique.
J Pharm Biomed Anal. 2004 Sep 21; 36(1):169-74.JP

Abstract

A micellar electrokinetic chromatography (MEKC) method was developed for the determination of nateglinide in animal plasma by on-line sweeping technique, in which plasma samples were simply deproteinized with acetonitrile, and analyzed with 16 mmol/L NaH2PO4 + 6 mmol/L Na2B4O7 + 60 mmol/L sodium dodecyl sulfate (SDS) (pH 7.14) as the running buffer, a fused-silica capillary as the separation tube, 21 kV as the running voltage and UV detection at 214 nm. Under these conditions, more than 100-fold enrichment of nateglinide was obtained with the good linear relation in the range of nateglinide plasma concentration 0.2-7 mg/L (R = 0.998). The method could be applied successfully to determine trace drugs in clinical analysis.

Authors+Show Affiliations

Yan H
Institute of Chemistry, Center for Molecular Science, Chinese Academy of Science, Beijing 100080, China.
Yang G
No affiliation info available
Qiao F
No affiliation info available
Chen Y
No affiliation info available

MeSH

AnimalsBuffersChromatography, Micellar Electrokinetic CapillaryCyclohexanesHydrogen-Ion ConcentrationNateglinidePhenylalanineRabbitsReproducibility of ResultsSensitivity and SpecificitySpecimen Handling

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15351062

Citation

Yan, Hongyuan, et al. "Determination of Nateglinide in Animal Plasma By Micellar Electrokinetic Chromatography and On-line Sweeping Technique." Journal of Pharmaceutical and Biomedical Analysis, vol. 36, no. 1, 2004, pp. 169-74.
Yan H, Yang G, Qiao F, et al. Determination of nateglinide in animal plasma by micellar electrokinetic chromatography and on-line sweeping technique. J Pharm Biomed Anal. 2004;36(1):169-74.
Yan, H., Yang, G., Qiao, F., & Chen, Y. (2004). Determination of nateglinide in animal plasma by micellar electrokinetic chromatography and on-line sweeping technique. Journal of Pharmaceutical and Biomedical Analysis, 36(1), 169-74.
Yan H, et al. Determination of Nateglinide in Animal Plasma By Micellar Electrokinetic Chromatography and On-line Sweeping Technique. J Pharm Biomed Anal. 2004 Sep 21;36(1):169-74. PubMed PMID: 15351062.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of nateglinide in animal plasma by micellar electrokinetic chromatography and on-line sweeping technique. AU - Yan,Hongyuan, AU - Yang,Gengliang, AU - Qiao,Fengxia, AU - Chen,Yi, PY - 2004/01/15/received PY - 2004/05/10/revised PY - 2004/05/12/accepted PY - 2004/9/8/pubmed PY - 2005/2/11/medline PY - 2004/9/8/entrez SP - 169 EP - 74 JF - Journal of pharmaceutical and biomedical analysis JO - J Pharm Biomed Anal VL - 36 IS - 1 N2 - A micellar electrokinetic chromatography (MEKC) method was developed for the determination of nateglinide in animal plasma by on-line sweeping technique, in which plasma samples were simply deproteinized with acetonitrile, and analyzed with 16 mmol/L NaH2PO4 + 6 mmol/L Na2B4O7 + 60 mmol/L sodium dodecyl sulfate (SDS) (pH 7.14) as the running buffer, a fused-silica capillary as the separation tube, 21 kV as the running voltage and UV detection at 214 nm. Under these conditions, more than 100-fold enrichment of nateglinide was obtained with the good linear relation in the range of nateglinide plasma concentration 0.2-7 mg/L (R = 0.998). The method could be applied successfully to determine trace drugs in clinical analysis. SN - 0731-7085 UR - https://www.unboundmedicine.com/medline/citation/15351062/Determination_of_nateglinide_in_animal_plasma_by_micellar_electrokinetic_chromatography_and_on_line_sweeping_technique_ DB - PRIME DP - Unbound Medicine ER -