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Glucose biosensor prepared by glucose oxidase encapsulated sol-gel and carbon-nanotube-modified basal plane pyrolytic graphite electrode.
Anal Biochem. 2004 Oct 01; 333(1):49-56.AB

Abstract

A new glucose biosensor has been fabricated by immobilizing glucose oxidase into a sol-gel composite at the surface of a basal plane pyrolytic graphite (bppg) electrode modified with multiwall carbon nanotube. First, the bppg electrode is subjected to abrasive immobilization of carbon nanotubes by gently rubbing the electrode surface on a filter paper supporting the carbon nanotubes. Second, the electrode surface is covered with a thin film of a sol-gel composite containing encapsulated glucose oxidase. The carbon nanotubes offer excellent electrocatalytic activity toward reduction and oxidation of hydrogen peroxide liberated in the enzymatic reaction between glucose oxidase and glucose, enabling sensitive determination of glucose. The amperometric detection of glucose is carried out at 0.3 V (vs saturated calomel electrode) in 0.05 M phosphate buffer solution (pH 7.4) with linear response range of 0.2-20 mM glucose, sensitivity of 196 nA/mM, and detection limit of 50 microM (S/N=3). The response time of the electrode is < 5s when it is stored dried at 4 degrees C, the sensor showed almost no change in the analytical performance after operation for 3 weeks. The present carbon nanotube sol-gel biocomposite glucose oxidase sensor showed excellent properties for the sensitive determination of glucose with good reproducibility, remarkable stability, and rapid response and in comparison to bulk modified composite biosensors the amounts of enzyme and carbon nanotube needed for electrode fabrication are dramatically decreased.

Authors+Show Affiliations

Department of Chemistry, Kurdistan University, P.O. Box 416, Sanandaj, Iran. absalimi@yahoo.comNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15351279

Citation

Salimi, Abdollah, et al. "Glucose Biosensor Prepared By Glucose Oxidase Encapsulated Sol-gel and Carbon-nanotube-modified Basal Plane Pyrolytic Graphite Electrode." Analytical Biochemistry, vol. 333, no. 1, 2004, pp. 49-56.
Salimi A, Compton RG, Hallaj R. Glucose biosensor prepared by glucose oxidase encapsulated sol-gel and carbon-nanotube-modified basal plane pyrolytic graphite electrode. Anal Biochem. 2004;333(1):49-56.
Salimi, A., Compton, R. G., & Hallaj, R. (2004). Glucose biosensor prepared by glucose oxidase encapsulated sol-gel and carbon-nanotube-modified basal plane pyrolytic graphite electrode. Analytical Biochemistry, 333(1), 49-56.
Salimi A, Compton RG, Hallaj R. Glucose Biosensor Prepared By Glucose Oxidase Encapsulated Sol-gel and Carbon-nanotube-modified Basal Plane Pyrolytic Graphite Electrode. Anal Biochem. 2004 Oct 1;333(1):49-56. PubMed PMID: 15351279.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Glucose biosensor prepared by glucose oxidase encapsulated sol-gel and carbon-nanotube-modified basal plane pyrolytic graphite electrode. AU - Salimi,Abdollah, AU - Compton,Richard G, AU - Hallaj,Rahman, PY - 2004/03/15/received PY - 2004/9/8/pubmed PY - 2005/3/25/medline PY - 2004/9/8/entrez SP - 49 EP - 56 JF - Analytical biochemistry JO - Anal Biochem VL - 333 IS - 1 N2 - A new glucose biosensor has been fabricated by immobilizing glucose oxidase into a sol-gel composite at the surface of a basal plane pyrolytic graphite (bppg) electrode modified with multiwall carbon nanotube. First, the bppg electrode is subjected to abrasive immobilization of carbon nanotubes by gently rubbing the electrode surface on a filter paper supporting the carbon nanotubes. Second, the electrode surface is covered with a thin film of a sol-gel composite containing encapsulated glucose oxidase. The carbon nanotubes offer excellent electrocatalytic activity toward reduction and oxidation of hydrogen peroxide liberated in the enzymatic reaction between glucose oxidase and glucose, enabling sensitive determination of glucose. The amperometric detection of glucose is carried out at 0.3 V (vs saturated calomel electrode) in 0.05 M phosphate buffer solution (pH 7.4) with linear response range of 0.2-20 mM glucose, sensitivity of 196 nA/mM, and detection limit of 50 microM (S/N=3). The response time of the electrode is < 5s when it is stored dried at 4 degrees C, the sensor showed almost no change in the analytical performance after operation for 3 weeks. The present carbon nanotube sol-gel biocomposite glucose oxidase sensor showed excellent properties for the sensitive determination of glucose with good reproducibility, remarkable stability, and rapid response and in comparison to bulk modified composite biosensors the amounts of enzyme and carbon nanotube needed for electrode fabrication are dramatically decreased. SN - 0003-2697 UR - https://www.unboundmedicine.com/medline/citation/15351279/Glucose_biosensor_prepared_by_glucose_oxidase_encapsulated_sol_gel_and_carbon_nanotube_modified_basal_plane_pyrolytic_graphite_electrode_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0003-2697(04)00572-X DB - PRIME DP - Unbound Medicine ER -