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Influence of multigeneration acclimation to copper on tolerance, energy reserves, and homeostasis of Daphnia magna straus.
Environ Toxicol Chem. 2004 Aug; 23(8):2029-37.ET

Abstract

A multigeneration acclimation experiment was performed with Daphnia magna exposed to copper to assess possible changes in tolerance and to establish the optimal concentration range (OCEE) of this species. The hypothesis was tested that as the bioavailable background concentration of an essential metal increases (within realistic limits), the natural tolerance (to the metal) of the acclimated/adapted organisms and communities will increase. During 18 months the daphnids were exposed to six different, environmentally relevant, copper background concentrations ranging between 0.5 and 100 microg Cu L(-1) (7 x 10(-15) and 3.7 x 10(-9) M Cu2+). An increase in acute (effect concentration resulting in 50% immobility: 48-h EC50) and chronic copper (effect concentration resulting in 50% or 10% reproduction reduction: 21-d EC50, 21-d EC10) tolerance was observed with increasing exposure concentration. The 48-h EC50 increased significantly from 204 +/- 24 microg Cu L(-1) to 320 +/- 43 microg Cu L(-1). A nonsignificant change from 48.0 (47.9-48.0) microg Cu L(-1) to 78.8 (66.3-93.6) microg Cu L(-1) was noted in the chronic toxicity assays. The optimal concentration range was assessed using different biological parameters (i.e., net reproduction [R0]), energy reserves (Ea), body length measurements, filtration rates, and body burdens. After three generations of acclimation the OCEE ranged between 1 and 35 microg Cu L(-1) (2 X 10(-14) to 80 x 10(-12) M Cu2+). Body burden measurements revealed an active copper regulation up to 35 microg Cu L(-1) (80 pM Cu2+). It can be concluded that acclimation of D. magna to copper does occur in laboratory experiments, even at realistic copper background concentrations (10(-11) - 10(-9) M Cu2+). However, it is suggested that this phenomenon is of less importance in the context of regulatory risk assessments. An optimal copper concentration range for D. magna was observed between 1 and 35 microg Cu L(-1) (10(-14) - 10(-11) M Cu2+), indicating that copper deficiency can occur in routine laboratory cultures.

Authors+Show Affiliations

Bossuyt BT
Laboratory of Environmental Toxicology and Aquatic Ecology, Ghent University, J. Plateaustraat 22, B-9000 Ghent, Belgium. bart.bossuyt@ugent.be
Janssen CR
No affiliation info available

MeSH

Adaptation, PhysiologicalAnimalsBiological AvailabilityBody BurdenCopperDaphniaDrug ResistanceEnvironmental ExposurePedigreeWater Pollutants

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15352494

Citation

Bossuyt, Bart T A., and Colin R. Janssen. "Influence of Multigeneration Acclimation to Copper On Tolerance, Energy Reserves, and Homeostasis of Daphnia Magna Straus." Environmental Toxicology and Chemistry, vol. 23, no. 8, 2004, pp. 2029-37.
Bossuyt BT, Janssen CR. Influence of multigeneration acclimation to copper on tolerance, energy reserves, and homeostasis of Daphnia magna straus. Environ Toxicol Chem. 2004;23(8):2029-37.
Bossuyt, B. T., & Janssen, C. R. (2004). Influence of multigeneration acclimation to copper on tolerance, energy reserves, and homeostasis of Daphnia magna straus. Environmental Toxicology and Chemistry, 23(8), 2029-37.
Bossuyt BT, Janssen CR. Influence of Multigeneration Acclimation to Copper On Tolerance, Energy Reserves, and Homeostasis of Daphnia Magna Straus. Environ Toxicol Chem. 2004;23(8):2029-37. PubMed PMID: 15352494.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Influence of multigeneration acclimation to copper on tolerance, energy reserves, and homeostasis of Daphnia magna straus. AU - Bossuyt,Bart T A, AU - Janssen,Colin R, PY - 2004/9/9/pubmed PY - 2004/11/13/medline PY - 2004/9/9/entrez SP - 2029 EP - 37 JF - Environmental toxicology and chemistry JO - Environ Toxicol Chem VL - 23 IS - 8 N2 - A multigeneration acclimation experiment was performed with Daphnia magna exposed to copper to assess possible changes in tolerance and to establish the optimal concentration range (OCEE) of this species. The hypothesis was tested that as the bioavailable background concentration of an essential metal increases (within realistic limits), the natural tolerance (to the metal) of the acclimated/adapted organisms and communities will increase. During 18 months the daphnids were exposed to six different, environmentally relevant, copper background concentrations ranging between 0.5 and 100 microg Cu L(-1) (7 x 10(-15) and 3.7 x 10(-9) M Cu2+). An increase in acute (effect concentration resulting in 50% immobility: 48-h EC50) and chronic copper (effect concentration resulting in 50% or 10% reproduction reduction: 21-d EC50, 21-d EC10) tolerance was observed with increasing exposure concentration. The 48-h EC50 increased significantly from 204 +/- 24 microg Cu L(-1) to 320 +/- 43 microg Cu L(-1). A nonsignificant change from 48.0 (47.9-48.0) microg Cu L(-1) to 78.8 (66.3-93.6) microg Cu L(-1) was noted in the chronic toxicity assays. The optimal concentration range was assessed using different biological parameters (i.e., net reproduction [R0]), energy reserves (Ea), body length measurements, filtration rates, and body burdens. After three generations of acclimation the OCEE ranged between 1 and 35 microg Cu L(-1) (2 X 10(-14) to 80 x 10(-12) M Cu2+). Body burden measurements revealed an active copper regulation up to 35 microg Cu L(-1) (80 pM Cu2+). It can be concluded that acclimation of D. magna to copper does occur in laboratory experiments, even at realistic copper background concentrations (10(-11) - 10(-9) M Cu2+). However, it is suggested that this phenomenon is of less importance in the context of regulatory risk assessments. An optimal copper concentration range for D. magna was observed between 1 and 35 microg Cu L(-1) (10(-14) - 10(-11) M Cu2+), indicating that copper deficiency can occur in routine laboratory cultures. SN - 0730-7268 UR - https://www.unboundmedicine.com/medline/citation/15352494/Influence_of_multigeneration_acclimation_to_copper_on_tolerance_energy_reserves_and_homeostasis_of_Daphnia_magna_straus_ DB - PRIME DP - Unbound Medicine ER -