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Some immune parameters in carp cyprinus Carpio susceptible and resistant to the haemoflagellate Trypanoplasma borreli.
Dis Aquat Organ. 2004 Jul 05; 60(1):41-8.DA

Abstract

The present study addresses aspects of the (specific) immune response of carp to the haemoflagellate Trypanoplasma borreli. Sera of resistant carp contained antibodies, which agglutinated the flagellates in vitro. When flagellates were incubated in fish sera from resistant carp, binding of antibodies to flagellates could be demonstrated by flow cytometry, and T. borreli were effectively killed. Heat-treatment of the sera prevented killing, indicating that complement activation is important for the control of a T. borreli infection. Sera of carp that were highly susceptible to infection with T. borreli contained no antibodies capable of binding to or killing the parasite. Furthermore, specific antibodies were not generated after experimental infection. This lack of antibody production in susceptible carp is not due to a general unresponsiveness of lymphoid cells, since peripheral blood leukocytes (PBL) from susceptible and resistant carp responded to mitogenic stimuli in vitro with lymphocyte proliferation in a similar manner. However, viable flagellates were significantly less able to stimulate proliferation of PBL from susceptible carp. In vitro-produced culture supernatants of freshly isolated PBL from both carp lines (but not those of head kidney cells) differentially modulated the mitogen-induced proliferation of PBL from susceptible and resistant carp. The supernatants enhanced the proliferation of leukocytes obtained from individuals from the same carp line, but suppressed the mitogen-induced proliferation of PBL from the other line tested. This indicates that lymphoid cells from susceptible and resistant carp differ in their spectrum of spontaneously produced immunomodulatory mediators. Whether this is decisive for a T. borreli-specific and successful immune response is discussed.

Authors+Show Affiliations

Scharsack JP
Fish Disease Research Unit, School of Veterinary Medicine, Hannover, Hannover, Germany.
Steinhagen D
No affiliation info available
Körting W
No affiliation info available
Wagner B
No affiliation info available
Leibold W
No affiliation info available
Schuberth HJ
No affiliation info available

MeSH

Analysis of VarianceAnimalsAntibodiesCarpsCulture MediaElectrophoresis, Polyacrylamide GelFish DiseasesFlow CytometryImmune SeraImmunity, InnateKinetoplastidaLeukocytesProtozoan InfectionsProtozoan Infections, Animal

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

15352523

Citation

Scharsack, J P., et al. "Some Immune Parameters in Carp Cyprinus Carpio Susceptible and Resistant to the Haemoflagellate Trypanoplasma Borreli." Diseases of Aquatic Organisms, vol. 60, no. 1, 2004, pp. 41-8.
Scharsack JP, Steinhagen D, Körting W, et al. Some immune parameters in carp cyprinus Carpio susceptible and resistant to the haemoflagellate Trypanoplasma borreli. Dis Aquat Organ. 2004;60(1):41-8.
Scharsack, J. P., Steinhagen, D., Körting, W., Wagner, B., Leibold, W., & Schuberth, H. J. (2004). Some immune parameters in carp cyprinus Carpio susceptible and resistant to the haemoflagellate Trypanoplasma borreli. Diseases of Aquatic Organisms, 60(1), 41-8.
Scharsack JP, et al. Some Immune Parameters in Carp Cyprinus Carpio Susceptible and Resistant to the Haemoflagellate Trypanoplasma Borreli. Dis Aquat Organ. 2004 Jul 5;60(1):41-8. PubMed PMID: 15352523.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Some immune parameters in carp cyprinus Carpio susceptible and resistant to the haemoflagellate Trypanoplasma borreli. AU - Scharsack,J P, AU - Steinhagen,D, AU - Körting,W, AU - Wagner,B, AU - Leibold,W, AU - Schuberth,H J, PY - 2004/9/9/pubmed PY - 2004/10/22/medline PY - 2004/9/9/entrez SP - 41 EP - 8 JF - Diseases of aquatic organisms JO - Dis Aquat Organ VL - 60 IS - 1 N2 - The present study addresses aspects of the (specific) immune response of carp to the haemoflagellate Trypanoplasma borreli. Sera of resistant carp contained antibodies, which agglutinated the flagellates in vitro. When flagellates were incubated in fish sera from resistant carp, binding of antibodies to flagellates could be demonstrated by flow cytometry, and T. borreli were effectively killed. Heat-treatment of the sera prevented killing, indicating that complement activation is important for the control of a T. borreli infection. Sera of carp that were highly susceptible to infection with T. borreli contained no antibodies capable of binding to or killing the parasite. Furthermore, specific antibodies were not generated after experimental infection. This lack of antibody production in susceptible carp is not due to a general unresponsiveness of lymphoid cells, since peripheral blood leukocytes (PBL) from susceptible and resistant carp responded to mitogenic stimuli in vitro with lymphocyte proliferation in a similar manner. However, viable flagellates were significantly less able to stimulate proliferation of PBL from susceptible carp. In vitro-produced culture supernatants of freshly isolated PBL from both carp lines (but not those of head kidney cells) differentially modulated the mitogen-induced proliferation of PBL from susceptible and resistant carp. The supernatants enhanced the proliferation of leukocytes obtained from individuals from the same carp line, but suppressed the mitogen-induced proliferation of PBL from the other line tested. This indicates that lymphoid cells from susceptible and resistant carp differ in their spectrum of spontaneously produced immunomodulatory mediators. Whether this is decisive for a T. borreli-specific and successful immune response is discussed. SN - 0177-5103 UR - https://www.unboundmedicine.com/medline/citation/15352523/Some_immune_parameters_in_carp_cyprinus_Carpio_susceptible_and_resistant_to_the_haemoflagellate_Trypanoplasma_borreli_ DB - PRIME DP - Unbound Medicine ER -