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Ewing sarcoma/peripheral primitive neuroectodermal tumor: adult abdominal tumors with an Ewing sarcoma gene rearrangement demonstrated by fluorescence in situ hybridization in paraffin sections.
Appl Immunohistochem Mol Morphol. 2004 Jun; 12(2):160-5.AI

Abstract

The differential diagnosis of small round cell tumors is exhaustive and requires ancillary studies. Relatively recently, fluorescence in situ hybridization (FISH) using probes for specific gene rearrangements has gained wide acceptance. This technique is particularly useful in the differential diagnosis of Ewing sarcoma/primitive neuroectodermal tumor (ES/PNET) and desmoplastic small round-cell tumor (DSRCT). In ES/PNET, the EWS gene is juxtaposed to the FLI-1 gene in 85% of cases and to the ERG gene in another 7% of cases; the EWS gene is juxtaposed to the WTI gene in DSRCT. Documentation of the EWS gene rearrangements in EWS/PNET has previously been demonstrated in frozen tissue. We report 2 unusual cases of EWS/PNET diagnosed in abdominal tumors in adults. Although the immunohistochemical results supported a diagnosis of ES/PNET, 1 case morphologically resembled DSRCT. The diagnosis in these 2 cases was confirmed by the FISH demonstration of EWS/FLI-1 gene fusion in paraffin-embedded tissue. Thus, the usefulness of FISH demonstration of an EWS gene rearrangement with these specific probes in such unusual cases is supported and is demonstrated in paraffin-embedded tissue.

Authors+Show Affiliations

Division of Hematopathology, Department of Pathology, St. Louis University Health Sciences Center, St. Louis, Missouri, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Case Reports
Journal Article

Language

eng

PubMed ID

15354743

Citation

Gardner, Laura J., et al. "Ewing Sarcoma/peripheral Primitive Neuroectodermal Tumor: Adult Abdominal Tumors With an Ewing Sarcoma Gene Rearrangement Demonstrated By Fluorescence in Situ Hybridization in Paraffin Sections." Applied Immunohistochemistry & Molecular Morphology : AIMM, vol. 12, no. 2, 2004, pp. 160-5.
Gardner LJ, Ayala AG, Monforte HL, et al. Ewing sarcoma/peripheral primitive neuroectodermal tumor: adult abdominal tumors with an Ewing sarcoma gene rearrangement demonstrated by fluorescence in situ hybridization in paraffin sections. Appl Immunohistochem Mol Morphol. 2004;12(2):160-5.
Gardner, L. J., Ayala, A. G., Monforte, H. L., & Dunphy, C. H. (2004). Ewing sarcoma/peripheral primitive neuroectodermal tumor: adult abdominal tumors with an Ewing sarcoma gene rearrangement demonstrated by fluorescence in situ hybridization in paraffin sections. Applied Immunohistochemistry & Molecular Morphology : AIMM, 12(2), 160-5.
Gardner LJ, et al. Ewing Sarcoma/peripheral Primitive Neuroectodermal Tumor: Adult Abdominal Tumors With an Ewing Sarcoma Gene Rearrangement Demonstrated By Fluorescence in Situ Hybridization in Paraffin Sections. Appl Immunohistochem Mol Morphol. 2004;12(2):160-5. PubMed PMID: 15354743.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ewing sarcoma/peripheral primitive neuroectodermal tumor: adult abdominal tumors with an Ewing sarcoma gene rearrangement demonstrated by fluorescence in situ hybridization in paraffin sections. AU - Gardner,Laura J, AU - Ayala,Alberto G, AU - Monforte,Hector L, AU - Dunphy,Cherie H, PY - 2004/9/10/pubmed PY - 2004/10/16/medline PY - 2004/9/10/entrez SP - 160 EP - 5 JF - Applied immunohistochemistry & molecular morphology : AIMM JO - Appl Immunohistochem Mol Morphol VL - 12 IS - 2 N2 - The differential diagnosis of small round cell tumors is exhaustive and requires ancillary studies. Relatively recently, fluorescence in situ hybridization (FISH) using probes for specific gene rearrangements has gained wide acceptance. This technique is particularly useful in the differential diagnosis of Ewing sarcoma/primitive neuroectodermal tumor (ES/PNET) and desmoplastic small round-cell tumor (DSRCT). In ES/PNET, the EWS gene is juxtaposed to the FLI-1 gene in 85% of cases and to the ERG gene in another 7% of cases; the EWS gene is juxtaposed to the WTI gene in DSRCT. Documentation of the EWS gene rearrangements in EWS/PNET has previously been demonstrated in frozen tissue. We report 2 unusual cases of EWS/PNET diagnosed in abdominal tumors in adults. Although the immunohistochemical results supported a diagnosis of ES/PNET, 1 case morphologically resembled DSRCT. The diagnosis in these 2 cases was confirmed by the FISH demonstration of EWS/FLI-1 gene fusion in paraffin-embedded tissue. Thus, the usefulness of FISH demonstration of an EWS gene rearrangement with these specific probes in such unusual cases is supported and is demonstrated in paraffin-embedded tissue. SN - 1541-2016 UR - https://www.unboundmedicine.com/medline/citation/15354743/Ewing_sarcoma/peripheral_primitive_neuroectodermal_tumor:_adult_abdominal_tumors_with_an_Ewing_sarcoma_gene_rearrangement_demonstrated_by_fluorescence_in_situ_hybridization_in_paraffin_sections_ L2 - https://doi.org/10.1097/00129039-200406000-00011 DB - PRIME DP - Unbound Medicine ER -