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[Epigallocatechin-3-gallate interferes with EBV-encoding AP-1 signal transduction pathway].
Zhonghua Zhong Liu Za Zhi. 2004 Jul; 26(7):393-7.ZZ

Abstract

OBJECTIVE

To elucidate the interference effect of Epigallocatechin-3-Gallate (EGCG) on targets of Activator Protein-1 (AP-1) signal transduction pathway activated by EB virus encoded latent membrane protein 1 in nasopharyngeal carcinoma (NPC) cell lines.

METHODS

Survival rate of cells was determined by MTT assay. AP-1 and CyclinD1 activation were analyzed by promoter luciferase reporter system. Nuclear translocation of JNK was analyzed by indirect immunofluorescence. Protein expression and phosphorylation were observed by Western blot.

RESULTS

EGCG inhibited the survival of CNE1 and CNE-LMP1 cells and the activity of AP-1 caused by LMP1 in CNE-LMP1 cells. EGCG also inhibited the nuclear translocation of JNK and the phosphorylation of c-Jun. It also inhibited cyclinD1 promoter activity and cyclinD1 expression.

CONCLUSION

EGCG inhibits AP-1, JNK, c-Jun and cyclinD1 which are key targets on AP-1 signal transduction pathway. The results may explain the molecular mechanism of action of EGCG against nasopharyngeal carcinoma.

Authors+Show Affiliations

Cancer Research Institute, Xiangya School of Medicine, Central South University, Changsha 410078, China. yan_68cn@yahoo.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

15355641

Citation

Zhao, Yan, et al. "[Epigallocatechin-3-gallate Interferes With EBV-encoding AP-1 Signal Transduction Pathway]." Zhonghua Zhong Liu Za Zhi [Chinese Journal of Oncology], vol. 26, no. 7, 2004, pp. 393-7.
Zhao Y, Wang H, Zhao XR, et al. [Epigallocatechin-3-gallate interferes with EBV-encoding AP-1 signal transduction pathway]. Zhonghua Zhong Liu Za Zhi. 2004;26(7):393-7.
Zhao, Y., Wang, H., Zhao, X. R., Luo, F. J., Tang, M., & Cao, Y. (2004). [Epigallocatechin-3-gallate interferes with EBV-encoding AP-1 signal transduction pathway]. Zhonghua Zhong Liu Za Zhi [Chinese Journal of Oncology], 26(7), 393-7.
Zhao Y, et al. [Epigallocatechin-3-gallate Interferes With EBV-encoding AP-1 Signal Transduction Pathway]. Zhonghua Zhong Liu Za Zhi. 2004;26(7):393-7. PubMed PMID: 15355641.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Epigallocatechin-3-gallate interferes with EBV-encoding AP-1 signal transduction pathway]. AU - Zhao,Yan, AU - Wang,Hai, AU - Zhao,Xiao-rong, AU - Luo,Fei-jun, AU - Tang,Min, AU - Cao,Ya, PY - 2004/9/10/pubmed PY - 2005/2/19/medline PY - 2004/9/10/entrez SP - 393 EP - 7 JF - Zhonghua zhong liu za zhi [Chinese journal of oncology] JO - Zhonghua Zhong Liu Za Zhi VL - 26 IS - 7 N2 - OBJECTIVE: To elucidate the interference effect of Epigallocatechin-3-Gallate (EGCG) on targets of Activator Protein-1 (AP-1) signal transduction pathway activated by EB virus encoded latent membrane protein 1 in nasopharyngeal carcinoma (NPC) cell lines. METHODS: Survival rate of cells was determined by MTT assay. AP-1 and CyclinD1 activation were analyzed by promoter luciferase reporter system. Nuclear translocation of JNK was analyzed by indirect immunofluorescence. Protein expression and phosphorylation were observed by Western blot. RESULTS: EGCG inhibited the survival of CNE1 and CNE-LMP1 cells and the activity of AP-1 caused by LMP1 in CNE-LMP1 cells. EGCG also inhibited the nuclear translocation of JNK and the phosphorylation of c-Jun. It also inhibited cyclinD1 promoter activity and cyclinD1 expression. CONCLUSION: EGCG inhibits AP-1, JNK, c-Jun and cyclinD1 which are key targets on AP-1 signal transduction pathway. The results may explain the molecular mechanism of action of EGCG against nasopharyngeal carcinoma. SN - 0253-3766 UR - https://www.unboundmedicine.com/medline/citation/15355641/[Epigallocatechin_3_gallate_interferes_with_EBV_encoding_AP_1_signal_transduction_pathway]_ L2 - http://journal.yiigle.com/LinkIn.do?linkin_type=pubmed&issn=0253-3766&year=2004&vol=26&issue=7&fpage=393 DB - PRIME DP - Unbound Medicine ER -