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[Prevalence and clinical significance of FLT3 internal tandem duplication mutation in acute leukemia].
Zhonghua Xue Ye Xue Za Zhi. 2004 Jul; 25(7):393-6.ZX

Abstract

OBJECTIVE

To evaluate the prevalence of FLT3 mutation-internal tandem duplication in acute leukemia (AL) patients and its significance.

METHODS

Genomic DNAs from 194 cases of AL were screened by polymerase chain reaction (PCR) and gel electrophoresis for FLT3-ITD mutations.

RESULTS

FLT3-ITDs were found in 37 (25.9%) of 143 de novo acute myeoloid leukemia (AML) patients, including 10/53 of M(2), 15/40 of M(3), 4/20 of M(4) and 8/23 of M(5). Significantly more FLT3 aberrations were found in AML M(3) and M(5) (P < 0.05). No FLT3-ITD was found in 25 acute lymphoid leukemia (ALL), 2 acute hybrid leukemia, 17 myelodysplastic syndromes and 7 chronic myelogenous leukemia in blast crisis. Sequence analysis of 5 cases with abnormal PCR electrophoretic patterns revealed that the ITDs were located within exon 14 from 21 bp to 60 bp, in 3 cases the ITD was a simple tandem duplication, and in 2 cases the ITD was tandem duplication with insertion, but all of the above ITD did not altered the FLT3 reading frame. FLT3-ITD was associated with a higher peripheral white cell count (P < 0.05) and a lower complete remission rate (P < 0.05), and was more prevalent in patients with normal karyotype (P < 0.05).

CONCLUSION

FLT3-ITD mutation occurs with a significant percentage in AML M(3) and M(5) patients. Sequences of the mutants are in frame mutation. FLT3-ITD mutation was associated with a higher peripheral white cell count and a lower complete remission rate.

Authors+Show Affiliations

State Key Laboratory of Experimental Hematology. Institute of Hematology and Blood Diseases Hospital, CAMS and PUMC, Tianjin 300020, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

15355689

Citation

Wang, Li-hong, et al. "[Prevalence and Clinical Significance of FLT3 Internal Tandem Duplication Mutation in Acute Leukemia]." Zhonghua Xue Ye Xue Za Zhi = Zhonghua Xueyexue Zazhi, vol. 25, no. 7, 2004, pp. 393-6.
Wang LH, Zhou CL, Zhang XW, et al. [Prevalence and clinical significance of FLT3 internal tandem duplication mutation in acute leukemia]. Zhonghua Xue Ye Xue Za Zhi. 2004;25(7):393-6.
Wang, L. H., Zhou, C. L., Zhang, X. W., Chen, S., Wang, M., & Wang, J. X. (2004). [Prevalence and clinical significance of FLT3 internal tandem duplication mutation in acute leukemia]. Zhonghua Xue Ye Xue Za Zhi = Zhonghua Xueyexue Zazhi, 25(7), 393-6.
Wang LH, et al. [Prevalence and Clinical Significance of FLT3 Internal Tandem Duplication Mutation in Acute Leukemia]. Zhonghua Xue Ye Xue Za Zhi. 2004;25(7):393-6. PubMed PMID: 15355689.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Prevalence and clinical significance of FLT3 internal tandem duplication mutation in acute leukemia]. AU - Wang,Li-hong, AU - Zhou,Chun-lin, AU - Zhang,Xin-wei, AU - Chen,Sen, AU - Wang,Min, AU - Wang,Jian-xiang, PY - 2004/9/10/pubmed PY - 2008/5/6/medline PY - 2004/9/10/entrez SP - 393 EP - 6 JF - Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi JO - Zhonghua Xue Ye Xue Za Zhi VL - 25 IS - 7 N2 - OBJECTIVE: To evaluate the prevalence of FLT3 mutation-internal tandem duplication in acute leukemia (AL) patients and its significance. METHODS: Genomic DNAs from 194 cases of AL were screened by polymerase chain reaction (PCR) and gel electrophoresis for FLT3-ITD mutations. RESULTS: FLT3-ITDs were found in 37 (25.9%) of 143 de novo acute myeoloid leukemia (AML) patients, including 10/53 of M(2), 15/40 of M(3), 4/20 of M(4) and 8/23 of M(5). Significantly more FLT3 aberrations were found in AML M(3) and M(5) (P < 0.05). No FLT3-ITD was found in 25 acute lymphoid leukemia (ALL), 2 acute hybrid leukemia, 17 myelodysplastic syndromes and 7 chronic myelogenous leukemia in blast crisis. Sequence analysis of 5 cases with abnormal PCR electrophoretic patterns revealed that the ITDs were located within exon 14 from 21 bp to 60 bp, in 3 cases the ITD was a simple tandem duplication, and in 2 cases the ITD was tandem duplication with insertion, but all of the above ITD did not altered the FLT3 reading frame. FLT3-ITD was associated with a higher peripheral white cell count (P < 0.05) and a lower complete remission rate (P < 0.05), and was more prevalent in patients with normal karyotype (P < 0.05). CONCLUSION: FLT3-ITD mutation occurs with a significant percentage in AML M(3) and M(5) patients. Sequences of the mutants are in frame mutation. FLT3-ITD mutation was associated with a higher peripheral white cell count and a lower complete remission rate. SN - 0253-2727 UR - https://www.unboundmedicine.com/medline/citation/15355689/[Prevalence_and_clinical_significance_of_FLT3_internal_tandem_duplication_mutation_in_acute_leukemia]_ L2 - http://journal.yiigle.com/LinkIn.do?linkin_type=pubmed&amp;issn=0253-2727&amp;year=2004&amp;vol=25&amp;issue=7&amp;fpage=393 DB - PRIME DP - Unbound Medicine ER -