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Phospholipase Cepsilon regulates ovulation in Caenorhabditis elegans.
Dev Biol. 2004 Oct 01; 274(1):201-10.DB

Abstract

Phospholipase Cepsilon (PLCepsilon) is a novel class of phosphoinositide-specific PLC with unknown physiological functions. Here, we present the first genetic analysis of PLCepsilon in an intact organism, the nematode Caenorhabditis elegans. Ovulation in C. elegans is dependent on an inositol 1,4,5-trisphosphate (IP(3)) signaling pathway activated by the receptor tyrosine kinase LET-23. We generated deletion mutants of the gene, plc-1, encoding C. elegans PLCepsilon. We observed a novel ovulation phenotype whereby oocytes are trapped in the spermatheca due to delayed dilation of the spermatheca-uterine valve. The expression of plc-1 in the adult spermatheca is consistent with its involvement in regulation of ovulation. On the other hand, we failed to observe genetic interaction of plc-1 with let-23-mediated IP(3) signaling pathway genes, suggesting a complex mechanism for control of ovulation.

Authors+Show Affiliations

Division of Cell Biology, Graduate School of Medicine, University of the Ryukyus, Nishihara-cho, Okinawa 903-0215, Japan. kariya@med.u-ryukyu.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

15355798

Citation

Kariya, Ken-Ichi, et al. "Phospholipase Cepsilon Regulates Ovulation in Caenorhabditis Elegans." Developmental Biology, vol. 274, no. 1, 2004, pp. 201-10.
Kariya K, Bui YK, Gao X, et al. Phospholipase Cepsilon regulates ovulation in Caenorhabditis elegans. Dev Biol. 2004;274(1):201-10.
Kariya, K., Bui, Y. K., Gao, X., Sternberg, P. W., & Kataoka, T. (2004). Phospholipase Cepsilon regulates ovulation in Caenorhabditis elegans. Developmental Biology, 274(1), 201-10.
Kariya K, et al. Phospholipase Cepsilon Regulates Ovulation in Caenorhabditis Elegans. Dev Biol. 2004 Oct 1;274(1):201-10. PubMed PMID: 15355798.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Phospholipase Cepsilon regulates ovulation in Caenorhabditis elegans. AU - Kariya,Ken-Ichi, AU - Bui,Yen Kim, AU - Gao,Xianlong, AU - Sternberg,Paul W, AU - Kataoka,Tohru, PY - 2004/03/19/received PY - 2004/06/24/revised PY - 2004/06/28/accepted PY - 2004/9/10/pubmed PY - 2004/11/4/medline PY - 2004/9/10/entrez SP - 201 EP - 10 JF - Developmental biology JO - Dev Biol VL - 274 IS - 1 N2 - Phospholipase Cepsilon (PLCepsilon) is a novel class of phosphoinositide-specific PLC with unknown physiological functions. Here, we present the first genetic analysis of PLCepsilon in an intact organism, the nematode Caenorhabditis elegans. Ovulation in C. elegans is dependent on an inositol 1,4,5-trisphosphate (IP(3)) signaling pathway activated by the receptor tyrosine kinase LET-23. We generated deletion mutants of the gene, plc-1, encoding C. elegans PLCepsilon. We observed a novel ovulation phenotype whereby oocytes are trapped in the spermatheca due to delayed dilation of the spermatheca-uterine valve. The expression of plc-1 in the adult spermatheca is consistent with its involvement in regulation of ovulation. On the other hand, we failed to observe genetic interaction of plc-1 with let-23-mediated IP(3) signaling pathway genes, suggesting a complex mechanism for control of ovulation. SN - 0012-1606 UR - https://www.unboundmedicine.com/medline/citation/15355798/Phospholipase_Cepsilon_regulates_ovulation_in_Caenorhabditis_elegans_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0012-1606(04)00480-4 DB - PRIME DP - Unbound Medicine ER -