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Anti-telomere antibodies in systemic lupus erythematosus (SLE): a comparison with five antinuclear antibody assays in 430 patients with SLE and other rheumatic diseases.
Ann Rheum Dis. 2004 Oct; 63(10):1250-4.AR

Abstract

OBJECTIVE

To investigate the prevalence and diagnostic significance of antibodies against telomeric DNA in systemic lupus erythematosus (SLE) and other autoimmune rheumatic diseases, and to make comparisons with five conventional anti-DNA or anti-nuclear antibody (ANA) assays.

METHODS

Antibodies to telomeres, which are highly repetitive sequences of DNA (TTAGGG/CCCTAA) at the end of eukaryotic chromosomes, were measured by an enzyme linked immunosorbent assay (ELISA) in 305 patients with SLE and 125 patients with other autoimmune rheumatic diseases (78 rheumatoid arthritis, 32 primary Sjögren's syndrome, eight mixed connective tissue disease, seven miscellaneous rheumatic diseases). Other assays used were two commercial ELISA assays for anti-dsDNA using calf thymus as antigen, Crithidialuciliae immunofluorescence, and radioimmunoassay (RIA) for anti-dsDNA and immunofluorescence using Hep-2 cells for ANA.

RESULTS

The prevalence of anti-telomere in SLE was 60%, v 5% in rheumatoid arthritis and 18% in other autoimmune rheumatic diseases. Specificity of anti-telomere for SLE was 91%; positive and negative predictive values were 95% and 46%, respectively. For anti-dsDNA by two ELISA assays using calf thymus as antigen, sensitivities were 69% and 29% and specificities 66% and 96%, respectively. Other anti-dsDNA assays had low sensitivities (RIA 43%, Crithidia immunofluorescence 13%). The association of anti-telomere with a history of nephritis in patients with SLE was stronger (p = 0.005) than by any other assay (p = 0.006-0.999). The correlations between the different assays were good (p<0.001 for all comparisons).

CONCLUSIONS

The new ELISA for anti-telomere antibodies using standardised human dsDNA as antigen is a sensitive and highly specific test for SLE.

Authors+Show Affiliations

Peijas Hospital, 01400 Vantaa, Finland.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15361381

Citation

Salonen, E M., et al. "Anti-telomere Antibodies in Systemic Lupus Erythematosus (SLE): a Comparison With Five Antinuclear Antibody Assays in 430 Patients With SLE and Other Rheumatic Diseases." Annals of the Rheumatic Diseases, vol. 63, no. 10, 2004, pp. 1250-4.
Salonen EM, Miettinen A, Walle TK, et al. Anti-telomere antibodies in systemic lupus erythematosus (SLE): a comparison with five antinuclear antibody assays in 430 patients with SLE and other rheumatic diseases. Ann Rheum Dis. 2004;63(10):1250-4.
Salonen, E. M., Miettinen, A., Walle, T. K., Koskenmies, S., Kere, J., & Julkunen, H. (2004). Anti-telomere antibodies in systemic lupus erythematosus (SLE): a comparison with five antinuclear antibody assays in 430 patients with SLE and other rheumatic diseases. Annals of the Rheumatic Diseases, 63(10), 1250-4.
Salonen EM, et al. Anti-telomere Antibodies in Systemic Lupus Erythematosus (SLE): a Comparison With Five Antinuclear Antibody Assays in 430 Patients With SLE and Other Rheumatic Diseases. Ann Rheum Dis. 2004;63(10):1250-4. PubMed PMID: 15361381.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Anti-telomere antibodies in systemic lupus erythematosus (SLE): a comparison with five antinuclear antibody assays in 430 patients with SLE and other rheumatic diseases. AU - Salonen,E M, AU - Miettinen,A, AU - Walle,T K, AU - Koskenmies,S, AU - Kere,J, AU - Julkunen,H, PY - 2004/9/14/pubmed PY - 2004/10/27/medline PY - 2004/9/14/entrez SP - 1250 EP - 4 JF - Annals of the rheumatic diseases JO - Ann. Rheum. Dis. VL - 63 IS - 10 N2 - OBJECTIVE: To investigate the prevalence and diagnostic significance of antibodies against telomeric DNA in systemic lupus erythematosus (SLE) and other autoimmune rheumatic diseases, and to make comparisons with five conventional anti-DNA or anti-nuclear antibody (ANA) assays. METHODS: Antibodies to telomeres, which are highly repetitive sequences of DNA (TTAGGG/CCCTAA) at the end of eukaryotic chromosomes, were measured by an enzyme linked immunosorbent assay (ELISA) in 305 patients with SLE and 125 patients with other autoimmune rheumatic diseases (78 rheumatoid arthritis, 32 primary Sjögren's syndrome, eight mixed connective tissue disease, seven miscellaneous rheumatic diseases). Other assays used were two commercial ELISA assays for anti-dsDNA using calf thymus as antigen, Crithidialuciliae immunofluorescence, and radioimmunoassay (RIA) for anti-dsDNA and immunofluorescence using Hep-2 cells for ANA. RESULTS: The prevalence of anti-telomere in SLE was 60%, v 5% in rheumatoid arthritis and 18% in other autoimmune rheumatic diseases. Specificity of anti-telomere for SLE was 91%; positive and negative predictive values were 95% and 46%, respectively. For anti-dsDNA by two ELISA assays using calf thymus as antigen, sensitivities were 69% and 29% and specificities 66% and 96%, respectively. Other anti-dsDNA assays had low sensitivities (RIA 43%, Crithidia immunofluorescence 13%). The association of anti-telomere with a history of nephritis in patients with SLE was stronger (p = 0.005) than by any other assay (p = 0.006-0.999). The correlations between the different assays were good (p<0.001 for all comparisons). CONCLUSIONS: The new ELISA for anti-telomere antibodies using standardised human dsDNA as antigen is a sensitive and highly specific test for SLE. SN - 0003-4967 UR - https://www.unboundmedicine.com/medline/citation/15361381/Anti_telomere_antibodies_in_systemic_lupus_erythematosus__SLE_:_a_comparison_with_five_antinuclear_antibody_assays_in_430_patients_with_SLE_and_other_rheumatic_diseases_ L2 - https://ard.bmj.com/cgi/pmidlookup?view=long&amp;pmid=15361381 DB - PRIME DP - Unbound Medicine ER -