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Label-free biosensing by surface plasmon resonance of nanoparticles on glass: optimization of nanoparticle size.
Anal Chem. 2004 Sep 15; 76(18):5370-8.AC

Abstract

The unique optical properties of noble metal nanoparticles have been used to design a label-free biosensor in a chip format. In this paper, we demonstrate that the size of gold nanoparticles significantly affects the sensitivity of the biosensor. Gold nanoparticles with diameters in the range of 12-48 nm were synthesized in solution and sensor chips were fabricated by chemisorption of these nanoparticles on amine-functionalized glass. Sensors fabricated from 39-nm-diameter gold nanoparticles exhibited maximum sensitivity to the change of the bulk refractive index and the largest "analytical volume", defined as the region around the nanoparticle within which a change in refractive index causes a change in the optical properties of the immobilized nanoparticles. The detection limit for streptavidin-biotin binding of a sensor fabricated from 39-nm-diameter nanoparticles was 20-fold better than a previously reported sensor fabricated from 13-nm-diameter gold nanoparticles. We also discuss several other factors that could improve the performance of the next generation of these immobilized metal nanoparticle sensors.

Authors+Show Affiliations

Department of Biomedical Engineering, Box 90281, Duke University, Durham, North Carolina 27708-0281, USA.No affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

15362894

Citation

Nath, Nidhi, and Ashutosh Chilkoti. "Label-free Biosensing By Surface Plasmon Resonance of Nanoparticles On Glass: Optimization of Nanoparticle Size." Analytical Chemistry, vol. 76, no. 18, 2004, pp. 5370-8.
Nath N, Chilkoti A. Label-free biosensing by surface plasmon resonance of nanoparticles on glass: optimization of nanoparticle size. Anal Chem. 2004;76(18):5370-8.
Nath, N., & Chilkoti, A. (2004). Label-free biosensing by surface plasmon resonance of nanoparticles on glass: optimization of nanoparticle size. Analytical Chemistry, 76(18), 5370-8.
Nath N, Chilkoti A. Label-free Biosensing By Surface Plasmon Resonance of Nanoparticles On Glass: Optimization of Nanoparticle Size. Anal Chem. 2004 Sep 15;76(18):5370-8. PubMed PMID: 15362894.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Label-free biosensing by surface plasmon resonance of nanoparticles on glass: optimization of nanoparticle size. AU - Nath,Nidhi, AU - Chilkoti,Ashutosh, PY - 2004/9/15/pubmed PY - 2006/5/25/medline PY - 2004/9/15/entrez SP - 5370 EP - 8 JF - Analytical chemistry JO - Anal Chem VL - 76 IS - 18 N2 - The unique optical properties of noble metal nanoparticles have been used to design a label-free biosensor in a chip format. In this paper, we demonstrate that the size of gold nanoparticles significantly affects the sensitivity of the biosensor. Gold nanoparticles with diameters in the range of 12-48 nm were synthesized in solution and sensor chips were fabricated by chemisorption of these nanoparticles on amine-functionalized glass. Sensors fabricated from 39-nm-diameter gold nanoparticles exhibited maximum sensitivity to the change of the bulk refractive index and the largest "analytical volume", defined as the region around the nanoparticle within which a change in refractive index causes a change in the optical properties of the immobilized nanoparticles. The detection limit for streptavidin-biotin binding of a sensor fabricated from 39-nm-diameter nanoparticles was 20-fold better than a previously reported sensor fabricated from 13-nm-diameter gold nanoparticles. We also discuss several other factors that could improve the performance of the next generation of these immobilized metal nanoparticle sensors. SN - 0003-2700 UR - https://www.unboundmedicine.com/medline/citation/15362894/Label_free_biosensing_by_surface_plasmon_resonance_of_nanoparticles_on_glass:_optimization_of_nanoparticle_size_ L2 - https://doi.org/10.1021/ac049741z DB - PRIME DP - Unbound Medicine ER -