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Shiga toxin-producing Escherichia coli in healthy young beef steers from Argentina: prevalence and virulence properties.
Int J Food Microbiol. 2004 Nov 01; 96(2):189-98.IJ

Abstract

Between July 1999 and December 2000, the prevalence of Shiga toxin-producing Escherichia coli (STEC) was established in 200 Argentine healthy young beef steers (14-16 months old) grown under local production systems with a feed grain period of 3-4 months, and the STEC strains isolated were examined in regard to their phenotypic and genotypic characteristics. Stool samples (n = 70) and rectal swabs (n = 130) were taken at the slaughterhouse level. By polymerase chain reaction (PCR), Shiga toxin (stx) gene sequences were detected in 69% of the samples. Eighty-six STEC strains were isolated from 39% of the animals. Serogroups identified, in order of frequency, were: O8 (16 strains), O113 (14), O103 (5), O91 (4), O171 (3), O174 (3), O25 (2), O112 (2), O145 (2), O2, O11, O104, O121, O128, O143, O146, O157. The most frequent serotype isolated was O8:H19 (12.9%). A total of 17 serotypes, including E. coli O157:H7 found in one animal (0.5%), have been previously associated with hemolytic uremic syndrome (HUS), bloody and non-bloody diarrhea in different countries, including Argentina. The prevalent genotype isolated was stx2 (51 of 86, 59.3%). Subtyping of stx2 variants showed the prevalence of stx2vh-b (25.6%) and stx2vh-a types (24.4%), and revealed the presence of an atypical stx2-v. Only 7.0% of STEC strains carried eae, and 33.7% harbored EHEC-hlyA gene. The full virulent genotype (stx/eae/EHEC-hlyA) was found to be present in 4 of the 86 (4.7%) STEC strains isolated. This research indicates that young steers from the main beef-producing area of Argentina are an important reservoir of STEC strains; however, its importance as agents of human diseases in our country has still to be established.

Authors+Show Affiliations

Instituto Tecnología de Alimentos, Centro de Investigación de Agroindustria, Instituto Nacional de Tecnología Agropecuaria, C.C. 77. C.P. (B1708WAB), Morón, Buenos Aires, Argentina.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15364473

Citation

Meichtri, Lelis, et al. "Shiga Toxin-producing Escherichia Coli in Healthy Young Beef Steers From Argentina: Prevalence and Virulence Properties." International Journal of Food Microbiology, vol. 96, no. 2, 2004, pp. 189-98.
Meichtri L, Miliwebsky E, Gioffré A, et al. Shiga toxin-producing Escherichia coli in healthy young beef steers from Argentina: prevalence and virulence properties. Int J Food Microbiol. 2004;96(2):189-98.
Meichtri, L., Miliwebsky, E., Gioffré, A., Chinen, I., Baschkier, A., Chillemi, G., Guth, B. E., Masana, M. O., Cataldi, A., Rodríguez, H. R., & Rivas, M. (2004). Shiga toxin-producing Escherichia coli in healthy young beef steers from Argentina: prevalence and virulence properties. International Journal of Food Microbiology, 96(2), 189-98.
Meichtri L, et al. Shiga Toxin-producing Escherichia Coli in Healthy Young Beef Steers From Argentina: Prevalence and Virulence Properties. Int J Food Microbiol. 2004 Nov 1;96(2):189-98. PubMed PMID: 15364473.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Shiga toxin-producing Escherichia coli in healthy young beef steers from Argentina: prevalence and virulence properties. AU - Meichtri,Lelis, AU - Miliwebsky,Elizabeth, AU - Gioffré,Andrea, AU - Chinen,Isabel, AU - Baschkier,Ariela, AU - Chillemi,Germán, AU - Guth,Beatriz E C, AU - Masana,Marcelo O, AU - Cataldi,Angel, AU - Rodríguez,H Ricardo, AU - Rivas,Marta, PY - 2003/01/05/received PY - 2003/12/10/revised PY - 2004/03/08/accepted PY - 2004/9/15/pubmed PY - 2004/12/16/medline PY - 2004/9/15/entrez SP - 189 EP - 98 JF - International journal of food microbiology JO - Int J Food Microbiol VL - 96 IS - 2 N2 - Between July 1999 and December 2000, the prevalence of Shiga toxin-producing Escherichia coli (STEC) was established in 200 Argentine healthy young beef steers (14-16 months old) grown under local production systems with a feed grain period of 3-4 months, and the STEC strains isolated were examined in regard to their phenotypic and genotypic characteristics. Stool samples (n = 70) and rectal swabs (n = 130) were taken at the slaughterhouse level. By polymerase chain reaction (PCR), Shiga toxin (stx) gene sequences were detected in 69% of the samples. Eighty-six STEC strains were isolated from 39% of the animals. Serogroups identified, in order of frequency, were: O8 (16 strains), O113 (14), O103 (5), O91 (4), O171 (3), O174 (3), O25 (2), O112 (2), O145 (2), O2, O11, O104, O121, O128, O143, O146, O157. The most frequent serotype isolated was O8:H19 (12.9%). A total of 17 serotypes, including E. coli O157:H7 found in one animal (0.5%), have been previously associated with hemolytic uremic syndrome (HUS), bloody and non-bloody diarrhea in different countries, including Argentina. The prevalent genotype isolated was stx2 (51 of 86, 59.3%). Subtyping of stx2 variants showed the prevalence of stx2vh-b (25.6%) and stx2vh-a types (24.4%), and revealed the presence of an atypical stx2-v. Only 7.0% of STEC strains carried eae, and 33.7% harbored EHEC-hlyA gene. The full virulent genotype (stx/eae/EHEC-hlyA) was found to be present in 4 of the 86 (4.7%) STEC strains isolated. This research indicates that young steers from the main beef-producing area of Argentina are an important reservoir of STEC strains; however, its importance as agents of human diseases in our country has still to be established. SN - 0168-1605 UR - https://www.unboundmedicine.com/medline/citation/15364473/Shiga_toxin_producing_Escherichia_coli_in_healthy_young_beef_steers_from_Argentina:_prevalence_and_virulence_properties_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0168160504001734 DB - PRIME DP - Unbound Medicine ER -