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[The role of costimulatory molecule 4-1BB and CD28 in activating of various T cell subsets from human peripheral blood].
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2004 Sep; 20(5):533-6.XB

Abstract

AIM

To explore the role of 4-1BB molecule in the activation and proliferation of CD4(+) T cells and CD8(+) T cells and compare it with that of CD28 molecule.

METHODS

Human peripheral blood mononuclear cells (PBMCs) were stimulated in-vitro with anti-CD3 mAb. Anti-4-1BB mAb and anti-CD80 mAb were added so as to block respectively costimulatory signals of 4-1BB/4-1BBL and CD28/B7-1. Flow cytometry was used to detect the proliferation ratio of CD4(+) T cells and CD8(+) T cells and the secretion of IFN-gamma.

RESULTS

When 4-1BB/4-1BBL and CD28/B7-1 signals were blocked by anti-4-1BBL mAb and anti-CD80 mAb, the proliferative response and the secretion of IFN-gamma by CD4(+) T cells and CD8(+) T cells were obviously decreased. In the group stimulated with anti-CD3 mAb only, the ratio of CD8/CD4 T cells was 1.98+/-0.06. In the groups blocked with anti-4-1BBL mAb and anti-CD80 mAb, the ratios were 0.96+/-0.03 and 2.69+/-0.16, respectively.

CONCLUSION

4-1BB molecule could provide a costimulatory signal for activation and proliferation of CD4(+) T cells and CD8(+) T cells. The costimulatory signal mediated by 4-1BB molecule could mainly induce the proliferative response of CD8(+) T cells, while that mediated by CD28 molecule could induce the activation of CD4(+) T cells mainly.

Authors+Show Affiliations

Department of Immunology, Bengbu Medical College, Bengbu 233003, China. jywu8191@sina.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

15367340

Citation

Wu, Jun-ying, et al. "[The Role of Costimulatory Molecule 4-1BB and CD28 in Activating of Various T Cell Subsets From Human Peripheral Blood]." Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi = Chinese Journal of Cellular and Molecular Immunology, vol. 20, no. 5, 2004, pp. 533-6.
Wu JY, Qian F, Lu HZ, et al. [The role of costimulatory molecule 4-1BB and CD28 in activating of various T cell subsets from human peripheral blood]. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2004;20(5):533-6.
Wu, J. Y., Qian, F., Lu, H. Z., Wang, W., & Li, B. Q. (2004). [The role of costimulatory molecule 4-1BB and CD28 in activating of various T cell subsets from human peripheral blood]. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi = Chinese Journal of Cellular and Molecular Immunology, 20(5), 533-6.
Wu JY, et al. [The Role of Costimulatory Molecule 4-1BB and CD28 in Activating of Various T Cell Subsets From Human Peripheral Blood]. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2004;20(5):533-6. PubMed PMID: 15367340.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [The role of costimulatory molecule 4-1BB and CD28 in activating of various T cell subsets from human peripheral blood]. AU - Wu,Jun-ying, AU - Qian,Feng, AU - Lu,He-zuo, AU - Wang,Wei, AU - Li,Bai-qing, PY - 2004/9/16/pubmed PY - 2006/4/6/medline PY - 2004/9/16/entrez SP - 533 EP - 6 JF - Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology JO - Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi VL - 20 IS - 5 N2 - AIM: To explore the role of 4-1BB molecule in the activation and proliferation of CD4(+) T cells and CD8(+) T cells and compare it with that of CD28 molecule. METHODS: Human peripheral blood mononuclear cells (PBMCs) were stimulated in-vitro with anti-CD3 mAb. Anti-4-1BB mAb and anti-CD80 mAb were added so as to block respectively costimulatory signals of 4-1BB/4-1BBL and CD28/B7-1. Flow cytometry was used to detect the proliferation ratio of CD4(+) T cells and CD8(+) T cells and the secretion of IFN-gamma. RESULTS: When 4-1BB/4-1BBL and CD28/B7-1 signals were blocked by anti-4-1BBL mAb and anti-CD80 mAb, the proliferative response and the secretion of IFN-gamma by CD4(+) T cells and CD8(+) T cells were obviously decreased. In the group stimulated with anti-CD3 mAb only, the ratio of CD8/CD4 T cells was 1.98+/-0.06. In the groups blocked with anti-4-1BBL mAb and anti-CD80 mAb, the ratios were 0.96+/-0.03 and 2.69+/-0.16, respectively. CONCLUSION: 4-1BB molecule could provide a costimulatory signal for activation and proliferation of CD4(+) T cells and CD8(+) T cells. The costimulatory signal mediated by 4-1BB molecule could mainly induce the proliferative response of CD8(+) T cells, while that mediated by CD28 molecule could induce the activation of CD4(+) T cells mainly. SN - 1007-8738 UR - https://www.unboundmedicine.com/medline/citation/15367340/[The_role_of_costimulatory_molecule_4_1BB_and_CD28_in_activating_of_various_T_cell_subsets_from_human_peripheral_blood]_ DB - PRIME DP - Unbound Medicine ER -