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Monitoring neurotransmitter release from isolated retinas using online microdialysis-capillary electrophoresis.
Anal Chem. 2004 Sep 01; 76(17):5069-74.AC

Abstract

Release of neurotransmitters and other primary amine-containing analytes from intact, isolated larval salamander (Ambystoma tigrinum) retinas maintained in a 6.5-microL perfusion chamber was monitored using online microdialysis-capillary electrophoresis with laser-induced fluorescence detection (CE-LIF). Primary amines were derivatized online with o-phthaldialdehyde (OPA) and beta-mercaptoethanol. With the use of overlapping injections, the perfusate was sampled every approximately 10 s. Although separation conditions were optimized using 20 mM hydroxypropyl-gamma-cyclodextrin (HP-gamma-CD) for a number of important neuromessengers including D- and L-serine, D- and L-asparate, glutamate, GABA, serotonin, dopamine, norepinephrine, and taurine, only glutamate (0.48 +/- 0.27 microM), GABA (0.25 +/- 0.12 microM), taurine (5.5 +/- 2.1 microM), and l-serine (2.8 +/- 1.0 microM) were identified in the perfusate. Elevated levels of glutamate, GABA, and taurine were detected during stimulation with 60 mM K+. This method is the first to directly sample multiple neurotransmitters from perfused, isolated retinas and to observe changes in efflux of these neurotransmitters as a result of pharmacological stimulation.

Authors+Show Affiliations

Department of Chemistry, University of Minnesota, Minneapolis, Minnesota 55455, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

15373444

Citation

O'Brien, Kylie B., et al. "Monitoring Neurotransmitter Release From Isolated Retinas Using Online Microdialysis-capillary Electrophoresis." Analytical Chemistry, vol. 76, no. 17, 2004, pp. 5069-74.
O'Brien KB, Esguerra M, Miller RF, et al. Monitoring neurotransmitter release from isolated retinas using online microdialysis-capillary electrophoresis. Anal Chem. 2004;76(17):5069-74.
O'Brien, K. B., Esguerra, M., Miller, R. F., & Bowser, M. T. (2004). Monitoring neurotransmitter release from isolated retinas using online microdialysis-capillary electrophoresis. Analytical Chemistry, 76(17), 5069-74.
O'Brien KB, et al. Monitoring Neurotransmitter Release From Isolated Retinas Using Online Microdialysis-capillary Electrophoresis. Anal Chem. 2004 Sep 1;76(17):5069-74. PubMed PMID: 15373444.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Monitoring neurotransmitter release from isolated retinas using online microdialysis-capillary electrophoresis. AU - O'Brien,Kylie B, AU - Esguerra,Manuel, AU - Miller,Robert F, AU - Bowser,Michael T, PY - 2004/9/18/pubmed PY - 2006/5/3/medline PY - 2004/9/18/entrez SP - 5069 EP - 74 JF - Analytical chemistry JO - Anal Chem VL - 76 IS - 17 N2 - Release of neurotransmitters and other primary amine-containing analytes from intact, isolated larval salamander (Ambystoma tigrinum) retinas maintained in a 6.5-microL perfusion chamber was monitored using online microdialysis-capillary electrophoresis with laser-induced fluorescence detection (CE-LIF). Primary amines were derivatized online with o-phthaldialdehyde (OPA) and beta-mercaptoethanol. With the use of overlapping injections, the perfusate was sampled every approximately 10 s. Although separation conditions were optimized using 20 mM hydroxypropyl-gamma-cyclodextrin (HP-gamma-CD) for a number of important neuromessengers including D- and L-serine, D- and L-asparate, glutamate, GABA, serotonin, dopamine, norepinephrine, and taurine, only glutamate (0.48 +/- 0.27 microM), GABA (0.25 +/- 0.12 microM), taurine (5.5 +/- 2.1 microM), and l-serine (2.8 +/- 1.0 microM) were identified in the perfusate. Elevated levels of glutamate, GABA, and taurine were detected during stimulation with 60 mM K+. This method is the first to directly sample multiple neurotransmitters from perfused, isolated retinas and to observe changes in efflux of these neurotransmitters as a result of pharmacological stimulation. SN - 0003-2700 UR - https://www.unboundmedicine.com/medline/citation/15373444/Monitoring_neurotransmitter_release_from_isolated_retinas_using_online_microdialysis_capillary_electrophoresis_ L2 - https://doi.org/10.1021/ac049822v DB - PRIME DP - Unbound Medicine ER -