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Surface-enhanced Raman spectroscopy for bacterial discrimination utilizing a scanning electron microscope with a Raman spectroscopy interface.
Anal Chem. 2004 Sep 01; 76(17):5198-202.AC

Abstract

Surface-enhanced Raman scattering (SERS) utilizing colloidal silver has already been shown to provide a rapid means of generating "whole-organism fingerprints" for use in bacterial identification and discrimination. However, one of the main drawbacks of the technique for the analysis of microbiological samples with optical Raman microspectroscopy has been the inability to acquire pre-emptively a region of the sample matrix where both the SERS substrate and biomass are both present. In this study, we introduce a Raman interface for scanning electron microscopy (SEM) and demonstrate the application of this technology to the reproducible and targeted collection of bacterial SERS spectra. In secondary electron mode, the SEM images clearly reveal regions of the sample matrix where the sodium borohydride-reduced silver colloidal particles are present, Stokes spectra collected from these regions are rich in vibrational bands, whereas spectra taken from other areas of the sample elicit a strong fluorescence response. Replicate SERS spectra were collected from two bacterial strains and show excellent reproducibility both by visual inspection and as demonstrated by principal components analysis on the whole SERS spectra.

Authors+Show Affiliations

Department of Chemistry, UMIST, P.O. Box 88, Sackville Street, Manchester M60 1QD, UK.No affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15373461

Citation

Jarvis, Roger M., et al. "Surface-enhanced Raman Spectroscopy for Bacterial Discrimination Utilizing a Scanning Electron Microscope With a Raman Spectroscopy Interface." Analytical Chemistry, vol. 76, no. 17, 2004, pp. 5198-202.
Jarvis RM, Brooker A, Goodacre R. Surface-enhanced Raman spectroscopy for bacterial discrimination utilizing a scanning electron microscope with a Raman spectroscopy interface. Anal Chem. 2004;76(17):5198-202.
Jarvis, R. M., Brooker, A., & Goodacre, R. (2004). Surface-enhanced Raman spectroscopy for bacterial discrimination utilizing a scanning electron microscope with a Raman spectroscopy interface. Analytical Chemistry, 76(17), 5198-202.
Jarvis RM, Brooker A, Goodacre R. Surface-enhanced Raman Spectroscopy for Bacterial Discrimination Utilizing a Scanning Electron Microscope With a Raman Spectroscopy Interface. Anal Chem. 2004 Sep 1;76(17):5198-202. PubMed PMID: 15373461.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Surface-enhanced Raman spectroscopy for bacterial discrimination utilizing a scanning electron microscope with a Raman spectroscopy interface. AU - Jarvis,Roger M, AU - Brooker,Alan, AU - Goodacre,Royston, PY - 2004/9/18/pubmed PY - 2006/5/3/medline PY - 2004/9/18/entrez SP - 5198 EP - 202 JF - Analytical chemistry JO - Anal Chem VL - 76 IS - 17 N2 - Surface-enhanced Raman scattering (SERS) utilizing colloidal silver has already been shown to provide a rapid means of generating "whole-organism fingerprints" for use in bacterial identification and discrimination. However, one of the main drawbacks of the technique for the analysis of microbiological samples with optical Raman microspectroscopy has been the inability to acquire pre-emptively a region of the sample matrix where both the SERS substrate and biomass are both present. In this study, we introduce a Raman interface for scanning electron microscopy (SEM) and demonstrate the application of this technology to the reproducible and targeted collection of bacterial SERS spectra. In secondary electron mode, the SEM images clearly reveal regions of the sample matrix where the sodium borohydride-reduced silver colloidal particles are present, Stokes spectra collected from these regions are rich in vibrational bands, whereas spectra taken from other areas of the sample elicit a strong fluorescence response. Replicate SERS spectra were collected from two bacterial strains and show excellent reproducibility both by visual inspection and as demonstrated by principal components analysis on the whole SERS spectra. SN - 0003-2700 UR - https://www.unboundmedicine.com/medline/citation/15373461/Surface_enhanced_Raman_spectroscopy_for_bacterial_discrimination_utilizing_a_scanning_electron_microscope_with_a_Raman_spectroscopy_interface_ L2 - https://doi.org/10.1021/ac049663f DB - PRIME DP - Unbound Medicine ER -