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Ryanodine contracture threshold times for diagnosis of malignant hyperthermia susceptibility: an experimental approach from a single laboratory.
J Clin Anesth. 2004 Aug; 16(5):353-7.JC

Abstract

STUDY OBJECTIVES

To define threshold times for ryanodine contracture testing (RCT) using skeletal muscle specimens from malignant hyperthermia-susceptible (MHS) and control individuals.

DESIGN

Prospective study.

SETTING

Malignant hyperthermia (MH) laboratory at a university hospital.

PATIENTS

8 patients with previous fulminant MH and 53 control patients undergoing in vitro contracture test (IVCT) for diagnosis of MH susceptibility.

INTERVENTIONS

Biopsies of the quadriceps femoris muscle were performed with a 3-in-1 nerve block, with spinal anesthesia, or with trigger-free general anesthesia.

MEASUREMENTS AND MAIN RESULTS

Patients were classified as MHS, MH normal (MHN), or MH equivocal (MHE) by the IVCT according to the protocol of the European MH Group (EMHG). Ryanodine 1 microM was added as a bolus to the organ bath to extra vital muscle specimens. Contracture levels were defined as: 1 = start of contracture (OT; min); 2 = time (min) to reach a contracture of 2 mN, and 3 = time (min) to reach a contracture of 10 mN. The effects of ryanodine on contracture responses were measured. Ryanodine induced contractures in all specimens. MHS specimens reached all defined contracture levels significantly sooner than did the controls. Ryanodine contracture test enables a clear discrimination of MHS specimens from controls at contracture levels of OT and 2 mN, whereas at 10 mN a small overlap was observed.

CONCLUSIONS

Using this test, which is an experimental approach from a single laboratory, an assignment to MHS or MHN is possible. To define contracture levels for RCT more precisely and to agree on commonly used thresholds, multicenter studies with larger numbers of patients are required.

Authors+Show Affiliations

Department of Anesthesiology, University Hospital Hamburg-Eppendorf, Hamburg, Germany. weisshorn@uke.uni-hamburg.deNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15374556

Citation

Weisshorn, Ralf, et al. "Ryanodine Contracture Threshold Times for Diagnosis of Malignant Hyperthermia Susceptibility: an Experimental Approach From a Single Laboratory." Journal of Clinical Anesthesia, vol. 16, no. 5, 2004, pp. 353-7.
Weisshorn R, Wappler F, Fiege M, et al. Ryanodine contracture threshold times for diagnosis of malignant hyperthermia susceptibility: an experimental approach from a single laboratory. J Clin Anesth. 2004;16(5):353-7.
Weisshorn, R., Wappler, F., Fiege, M., Gerbershagen, M. U., Kolodzie, K., Alberts, P., Horn, E. P., & Schulte Am Esch, J. (2004). Ryanodine contracture threshold times for diagnosis of malignant hyperthermia susceptibility: an experimental approach from a single laboratory. Journal of Clinical Anesthesia, 16(5), 353-7.
Weisshorn R, et al. Ryanodine Contracture Threshold Times for Diagnosis of Malignant Hyperthermia Susceptibility: an Experimental Approach From a Single Laboratory. J Clin Anesth. 2004;16(5):353-7. PubMed PMID: 15374556.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ryanodine contracture threshold times for diagnosis of malignant hyperthermia susceptibility: an experimental approach from a single laboratory. AU - Weisshorn,Ralf, AU - Wappler,Frank, AU - Fiege,Marko, AU - Gerbershagen,Mark U, AU - Kolodzie,Kerstin, AU - Alberts,Patrick, AU - Horn,Ernst-Peter, AU - Schulte Am Esch,Jochen, PY - 2003/05/20/received PY - 2003/09/25/revised PY - 2003/09/25/accepted PY - 2004/9/18/pubmed PY - 2005/1/13/medline PY - 2004/9/18/entrez SP - 353 EP - 7 JF - Journal of clinical anesthesia JO - J Clin Anesth VL - 16 IS - 5 N2 - STUDY OBJECTIVES: To define threshold times for ryanodine contracture testing (RCT) using skeletal muscle specimens from malignant hyperthermia-susceptible (MHS) and control individuals. DESIGN: Prospective study. SETTING: Malignant hyperthermia (MH) laboratory at a university hospital. PATIENTS: 8 patients with previous fulminant MH and 53 control patients undergoing in vitro contracture test (IVCT) for diagnosis of MH susceptibility. INTERVENTIONS: Biopsies of the quadriceps femoris muscle were performed with a 3-in-1 nerve block, with spinal anesthesia, or with trigger-free general anesthesia. MEASUREMENTS AND MAIN RESULTS: Patients were classified as MHS, MH normal (MHN), or MH equivocal (MHE) by the IVCT according to the protocol of the European MH Group (EMHG). Ryanodine 1 microM was added as a bolus to the organ bath to extra vital muscle specimens. Contracture levels were defined as: 1 = start of contracture (OT; min); 2 = time (min) to reach a contracture of 2 mN, and 3 = time (min) to reach a contracture of 10 mN. The effects of ryanodine on contracture responses were measured. Ryanodine induced contractures in all specimens. MHS specimens reached all defined contracture levels significantly sooner than did the controls. Ryanodine contracture test enables a clear discrimination of MHS specimens from controls at contracture levels of OT and 2 mN, whereas at 10 mN a small overlap was observed. CONCLUSIONS: Using this test, which is an experimental approach from a single laboratory, an assignment to MHS or MHN is possible. To define contracture levels for RCT more precisely and to agree on commonly used thresholds, multicenter studies with larger numbers of patients are required. SN - 0952-8180 UR - https://www.unboundmedicine.com/medline/citation/15374556/Ryanodine_contracture_threshold_times_for_diagnosis_of_malignant_hyperthermia_susceptibility:_an_experimental_approach_from_a_single_laboratory_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0952818004000777 DB - PRIME DP - Unbound Medicine ER -