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Genomic plasticity of the causative agent of melioidosis, Burkholderia pseudomallei.
Proc Natl Acad Sci U S A. 2004 Sep 28; 101(39):14240-5.PN

Abstract

Burkholderia pseudomallei is a recognized biothreat agent and the causative agent of melioidosis. This Gram-negative bacterium exists as a soil saprophyte in melioidosis-endemic areas of the world and accounts for 20% of community-acquired septicaemias in northeastern Thailand where half of those affected die. Here we report the complete genome of B. pseudomallei, which is composed of two chromosomes of 4.07 megabase pairs and 3.17 megabase pairs, showing significant functional partitioning of genes between them. The large chromosome encodes many of the core functions associated with central metabolism and cell growth, whereas the small chromosome carries more accessory functions associated with adaptation and survival in different niches. Genomic comparisons with closely and more distantly related bacteria revealed a greater level of gene order conservation and a greater number of orthologous genes on the large chromosome, suggesting that the two replicons have distinct evolutionary origins. A striking feature of the genome was the presence of 16 genomic islands (GIs) that together made up 6.1% of the genome. Further analysis revealed these islands to be variably present in a collection of invasive and soil isolates but entirely absent from the clonally related organism B. mallei. We propose that variable horizontal gene acquisition by B. pseudomallei is an important feature of recent genetic evolution and that this has resulted in a genetically diverse pathogenic species.

Authors+Show Affiliations

Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, United Kingdom.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15377794

Citation

Holden, Matthew T G., et al. "Genomic Plasticity of the Causative Agent of Melioidosis, Burkholderia Pseudomallei." Proceedings of the National Academy of Sciences of the United States of America, vol. 101, no. 39, 2004, pp. 14240-5.
Holden MT, Titball RW, Peacock SJ, et al. Genomic plasticity of the causative agent of melioidosis, Burkholderia pseudomallei. Proc Natl Acad Sci U S A. 2004;101(39):14240-5.
Holden, M. T., Titball, R. W., Peacock, S. J., Cerdeño-Tárraga, A. M., Atkins, T., Crossman, L. C., Pitt, T., Churcher, C., Mungall, K., Bentley, S. D., Sebaihia, M., Thomson, N. R., Bason, N., Beacham, I. R., Brooks, K., Brown, K. A., Brown, N. F., Challis, G. L., Cherevach, I., ... Parkhill, J. (2004). Genomic plasticity of the causative agent of melioidosis, Burkholderia pseudomallei. Proceedings of the National Academy of Sciences of the United States of America, 101(39), 14240-5.
Holden MT, et al. Genomic Plasticity of the Causative Agent of Melioidosis, Burkholderia Pseudomallei. Proc Natl Acad Sci U S A. 2004 Sep 28;101(39):14240-5. PubMed PMID: 15377794.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genomic plasticity of the causative agent of melioidosis, Burkholderia pseudomallei. AU - Holden,Matthew T G, AU - Titball,Richard W, AU - Peacock,Sharon J, AU - Cerdeño-Tárraga,Ana M, AU - Atkins,Timothy, AU - Crossman,Lisa C, AU - Pitt,Tyrone, AU - Churcher,Carol, AU - Mungall,Karen, AU - Bentley,Stephen D, AU - Sebaihia,Mohammed, AU - Thomson,Nicholas R, AU - Bason,Nathalie, AU - Beacham,Ifor R, AU - Brooks,Karen, AU - Brown,Katherine A, AU - Brown,Nat F, AU - Challis,Greg L, AU - Cherevach,Inna, AU - Chillingworth,Tracy, AU - Cronin,Ann, AU - Crossett,Ben, AU - Davis,Paul, AU - DeShazer,David, AU - Feltwell,Theresa, AU - Fraser,Audrey, AU - Hance,Zahra, AU - Hauser,Heidi, AU - Holroyd,Simon, AU - Jagels,Kay, AU - Keith,Karen E, AU - Maddison,Mark, AU - Moule,Sharon, AU - Price,Claire, AU - Quail,Michael A, AU - Rabbinowitsch,Ester, AU - Rutherford,Kim, AU - Sanders,Mandy, AU - Simmonds,Mark, AU - Songsivilai,Sirirurg, AU - Stevens,Kim, AU - Tumapa,Sarinna, AU - Vesaratchavest,Monkgol, AU - Whitehead,Sally, AU - Yeats,Corin, AU - Barrell,Bart G, AU - Oyston,Petra C F, AU - Parkhill,Julian, Y1 - 2004/09/17/ PY - 2004/9/21/pubmed PY - 2005/2/8/medline PY - 2004/9/21/entrez SP - 14240 EP - 5 JF - Proceedings of the National Academy of Sciences of the United States of America JO - Proc Natl Acad Sci U S A VL - 101 IS - 39 N2 - Burkholderia pseudomallei is a recognized biothreat agent and the causative agent of melioidosis. This Gram-negative bacterium exists as a soil saprophyte in melioidosis-endemic areas of the world and accounts for 20% of community-acquired septicaemias in northeastern Thailand where half of those affected die. Here we report the complete genome of B. pseudomallei, which is composed of two chromosomes of 4.07 megabase pairs and 3.17 megabase pairs, showing significant functional partitioning of genes between them. The large chromosome encodes many of the core functions associated with central metabolism and cell growth, whereas the small chromosome carries more accessory functions associated with adaptation and survival in different niches. Genomic comparisons with closely and more distantly related bacteria revealed a greater level of gene order conservation and a greater number of orthologous genes on the large chromosome, suggesting that the two replicons have distinct evolutionary origins. A striking feature of the genome was the presence of 16 genomic islands (GIs) that together made up 6.1% of the genome. Further analysis revealed these islands to be variably present in a collection of invasive and soil isolates but entirely absent from the clonally related organism B. mallei. We propose that variable horizontal gene acquisition by B. pseudomallei is an important feature of recent genetic evolution and that this has resulted in a genetically diverse pathogenic species. SN - 0027-8424 UR - https://www.unboundmedicine.com/medline/citation/15377794/Genomic_plasticity_of_the_causative_agent_of_melioidosis_Burkholderia_pseudomallei_ L2 - http://www.pnas.org/cgi/pmidlookup?view=long&pmid=15377794 DB - PRIME DP - Unbound Medicine ER -