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CD22 regulates B lymphocyte function in vivo through both ligand-dependent and ligand-independent mechanisms.
Nat Immunol. 2004 Oct; 5(10):1078-87.NI

Abstract

The interaction of CD22 with alpha2,6-linked sialic acid ligands has been widely proposed to regulate B lymphocyte function and migration. Here, we generated gene-targeted mice that express mutant CD22 molecules that do not interact with these ligands. CD22 ligand binding regulated the expression of cell surface CD22, immunoglobulin M and major histocompatibility complex class II on mature B cells, maintenance of the marginal zone B cell population, optimal B cell antigen receptor-induced proliferation, and B cell turnover rates. However, CD22 negative regulation of calcium mobilization after B cell antigen receptor ligation, CD22 phosphorylation, recruitment of SHP-1 to CD22 and B cell migration did not require CD22 ligand engagement. These observations resolve longstanding questions regarding the physiological importance of CD22 ligand binding in the regulation of B cell function in vivo.

Authors+Show Affiliations

Department of Immunology, Duke University Medical Center, Durham, North Carolina 27710, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

15378059

Citation

Poe, Jonathan C., et al. "CD22 Regulates B Lymphocyte Function in Vivo Through Both Ligand-dependent and Ligand-independent Mechanisms." Nature Immunology, vol. 5, no. 10, 2004, pp. 1078-87.
Poe JC, Fujimoto Y, Hasegawa M, et al. CD22 regulates B lymphocyte function in vivo through both ligand-dependent and ligand-independent mechanisms. Nat Immunol. 2004;5(10):1078-87.
Poe, J. C., Fujimoto, Y., Hasegawa, M., Haas, K. M., Miller, A. S., Sanford, I. G., Bock, C. B., Fujimoto, M., & Tedder, T. F. (2004). CD22 regulates B lymphocyte function in vivo through both ligand-dependent and ligand-independent mechanisms. Nature Immunology, 5(10), 1078-87.
Poe JC, et al. CD22 Regulates B Lymphocyte Function in Vivo Through Both Ligand-dependent and Ligand-independent Mechanisms. Nat Immunol. 2004;5(10):1078-87. PubMed PMID: 15378059.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - CD22 regulates B lymphocyte function in vivo through both ligand-dependent and ligand-independent mechanisms. AU - Poe,Jonathan C, AU - Fujimoto,Yoko, AU - Hasegawa,Minoru, AU - Haas,Karen M, AU - Miller,Ann S, AU - Sanford,Isaac G, AU - Bock,Cheryl B, AU - Fujimoto,Manabu, AU - Tedder,Thomas F, Y1 - 2004/09/19/ PY - 2004/03/31/received PY - 2004/08/25/accepted PY - 2004/9/21/pubmed PY - 2004/10/22/medline PY - 2004/9/21/entrez SP - 1078 EP - 87 JF - Nature immunology JO - Nat Immunol VL - 5 IS - 10 N2 - The interaction of CD22 with alpha2,6-linked sialic acid ligands has been widely proposed to regulate B lymphocyte function and migration. Here, we generated gene-targeted mice that express mutant CD22 molecules that do not interact with these ligands. CD22 ligand binding regulated the expression of cell surface CD22, immunoglobulin M and major histocompatibility complex class II on mature B cells, maintenance of the marginal zone B cell population, optimal B cell antigen receptor-induced proliferation, and B cell turnover rates. However, CD22 negative regulation of calcium mobilization after B cell antigen receptor ligation, CD22 phosphorylation, recruitment of SHP-1 to CD22 and B cell migration did not require CD22 ligand engagement. These observations resolve longstanding questions regarding the physiological importance of CD22 ligand binding in the regulation of B cell function in vivo. SN - 1529-2908 UR - https://www.unboundmedicine.com/medline/citation/15378059/CD22_regulates_B_lymphocyte_function_in_vivo_through_both_ligand_dependent_and_ligand_independent_mechanisms_ L2 - https://doi.org/10.1038/ni1121 DB - PRIME DP - Unbound Medicine ER -