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Comparison of laser-induced dissociation and high-energy collision-induced dissociation using matrix-assisted laser desorption/ionization tandem time-of-flight (MALDI-TOF/TOF) for peptide and protein identification.
Rapid Commun Mass Spectrom. 2004; 18(18):2093-105.RC

Abstract

The fragmentation of peptides under laser-induced dissociation (LID) as well as high-energy collision-induced dissociation (CID) conditions has been investigated. The effect of the different fragmentation mechanisms on the formation of specific fragment ion types and the usability of the resulting spectra, e.g. for high-throughput protein identification, has been evaluated. Also, basic investigations on the influence of the matrix, as well as laser fluence, on the fragment ion formation and the consequences in the spectral appearance are discussed. The preconditions for obtaining 'pure' CID spectra on matrix-assisted laser desorption/ionization tandem time-of-flight (MALDI-TOF/TOF) instruments are evaluated and discussed as well as the differences between LID and CID in the resulting fragment ion types. While containing a wealth of information due to additional fragment ions in comparison with LID, CID spectra are significantly more complex than LID spectra and, due to different fragmentation patterns, the CID spectra are of limited use for protein identification, even under optimized parameter settings, due to significantly lower scores for the individual spectra. Conditions for optimal results regarding protein identification using MALDI-TOF/TOF instruments have been evaluated. For database searches using tandem mass spectrometric data, the use of LID as fragmentation technique in combination with parameter settings supporting the use of internal fragment ions turned out to yield the optimal results.

Authors+Show Affiliations

Bruker Daltonik GmbH, Leipzig, Germany. mma@bdal.deNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Evaluation Study
Journal Article
Validation Study

Language

eng

PubMed ID

15378722

Citation

Macht, Marcus, et al. "Comparison of Laser-induced Dissociation and High-energy Collision-induced Dissociation Using Matrix-assisted Laser Desorption/ionization Tandem Time-of-flight (MALDI-TOF/TOF) for Peptide and Protein Identification." Rapid Communications in Mass Spectrometry : RCM, vol. 18, no. 18, 2004, pp. 2093-105.
Macht M, Asperger A, Deininger SO. Comparison of laser-induced dissociation and high-energy collision-induced dissociation using matrix-assisted laser desorption/ionization tandem time-of-flight (MALDI-TOF/TOF) for peptide and protein identification. Rapid Commun Mass Spectrom. 2004;18(18):2093-105.
Macht, M., Asperger, A., & Deininger, S. O. (2004). Comparison of laser-induced dissociation and high-energy collision-induced dissociation using matrix-assisted laser desorption/ionization tandem time-of-flight (MALDI-TOF/TOF) for peptide and protein identification. Rapid Communications in Mass Spectrometry : RCM, 18(18), 2093-105.
Macht M, Asperger A, Deininger SO. Comparison of Laser-induced Dissociation and High-energy Collision-induced Dissociation Using Matrix-assisted Laser Desorption/ionization Tandem Time-of-flight (MALDI-TOF/TOF) for Peptide and Protein Identification. Rapid Commun Mass Spectrom. 2004;18(18):2093-105. PubMed PMID: 15378722.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparison of laser-induced dissociation and high-energy collision-induced dissociation using matrix-assisted laser desorption/ionization tandem time-of-flight (MALDI-TOF/TOF) for peptide and protein identification. AU - Macht,Marcus, AU - Asperger,Arndt, AU - Deininger,Sören-Oliver, PY - 2004/9/21/pubmed PY - 2004/11/13/medline PY - 2004/9/21/entrez SP - 2093 EP - 105 JF - Rapid communications in mass spectrometry : RCM JO - Rapid Commun Mass Spectrom VL - 18 IS - 18 N2 - The fragmentation of peptides under laser-induced dissociation (LID) as well as high-energy collision-induced dissociation (CID) conditions has been investigated. The effect of the different fragmentation mechanisms on the formation of specific fragment ion types and the usability of the resulting spectra, e.g. for high-throughput protein identification, has been evaluated. Also, basic investigations on the influence of the matrix, as well as laser fluence, on the fragment ion formation and the consequences in the spectral appearance are discussed. The preconditions for obtaining 'pure' CID spectra on matrix-assisted laser desorption/ionization tandem time-of-flight (MALDI-TOF/TOF) instruments are evaluated and discussed as well as the differences between LID and CID in the resulting fragment ion types. While containing a wealth of information due to additional fragment ions in comparison with LID, CID spectra are significantly more complex than LID spectra and, due to different fragmentation patterns, the CID spectra are of limited use for protein identification, even under optimized parameter settings, due to significantly lower scores for the individual spectra. Conditions for optimal results regarding protein identification using MALDI-TOF/TOF instruments have been evaluated. For database searches using tandem mass spectrometric data, the use of LID as fragmentation technique in combination with parameter settings supporting the use of internal fragment ions turned out to yield the optimal results. SN - 0951-4198 UR - https://www.unboundmedicine.com/medline/citation/15378722/Comparison_of_laser_induced_dissociation_and_high_energy_collision_induced_dissociation_using_matrix_assisted_laser_desorption/ionization_tandem_time_of_flight__MALDI_TOF/TOF__for_peptide_and_protein_identification_ L2 - https://doi.org/10.1002/rcm.1589 DB - PRIME DP - Unbound Medicine ER -