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Detection of Mycoplasma genitalium, Mycoplasma hominis, Ureaplasma parvum (biovar 1) and Ureaplasma urealyticum (biovar 2) in patients with non-gonococcal urethritis using polymerase chain reaction-microtiter plate hybridization.
Int J Urol 2004; 11(9):750-4IJ

Abstract

AIM

To use polymerase chain reaction (PCR)-microtiter plate hybridization assays to detect Mycoplasma genitalium, Mycoplasma hominis, Ureaplasma parvum (biovar 1) and Ureaplasma urealyticum (biovar 2) in first-voided urine specimens from patients with non-gonococcal urethritis (NGU).

METHODS

A total of 153 male patients with NGU, who visited one of 24 clinics in Japan, were recruited for this study. All were examined using PCR-microtiter plate hybridization assays for the presence of M. genitalium, M. hominis, U. parvum (biovar 1) and U. urealyticum (biovar 2) in first-voided urine specimens. They were also examined for the presence of Chlamydia trachomatis.

RESULTS

Of these 153 patients, 73 (47.7%) were positive for C. trachomatis. Overall, the prevalence was 17.0% for M. genitalium, 16.3% for U. urealyticum (biovar 2), 7.8% for U. parvum (biovar 1) and 2.6% for M. hominis. In the 80 patients with non-chlamydial NGU, the prevalence of M. genitalium, U. urealyticum (biovar 2), U. parvum (biovar 1) and M. hominis was 23.8%, 18.8%, 8.8% and 2.6%, respectively.

CONCLUSIONS

This study shows the prevalence of mycoplasmas and ureaplasmas in NGU in Japan. M. genitalium and U. urealyticum (biovar 2) might be pathogens of NGU and could be associated with persistent and recurrent urethritis. When patients with NGU are treated, such pathogens should be taken into account. This PCR-microtiter plate hybridization assay provides a useful method for diagnosing NGU caused by M. genitalium and U. urealyticum (biovar 2).

Authors+Show Affiliations

Japanese Research Group for UTI, Kitakyushu, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15379939

Citation

Maeda, Shin-ichi, et al. "Detection of Mycoplasma Genitalium, Mycoplasma Hominis, Ureaplasma Parvum (biovar 1) and Ureaplasma Urealyticum (biovar 2) in Patients With Non-gonococcal Urethritis Using Polymerase Chain Reaction-microtiter Plate Hybridization." International Journal of Urology : Official Journal of the Japanese Urological Association, vol. 11, no. 9, 2004, pp. 750-4.
Maeda S, Deguchi T, Ishiko H, et al. Detection of Mycoplasma genitalium, Mycoplasma hominis, Ureaplasma parvum (biovar 1) and Ureaplasma urealyticum (biovar 2) in patients with non-gonococcal urethritis using polymerase chain reaction-microtiter plate hybridization. Int J Urol. 2004;11(9):750-4.
Maeda, S., Deguchi, T., Ishiko, H., Matsumoto, T., Naito, S., Kumon, H., ... Kamidono, S. (2004). Detection of Mycoplasma genitalium, Mycoplasma hominis, Ureaplasma parvum (biovar 1) and Ureaplasma urealyticum (biovar 2) in patients with non-gonococcal urethritis using polymerase chain reaction-microtiter plate hybridization. International Journal of Urology : Official Journal of the Japanese Urological Association, 11(9), pp. 750-4.
Maeda S, et al. Detection of Mycoplasma Genitalium, Mycoplasma Hominis, Ureaplasma Parvum (biovar 1) and Ureaplasma Urealyticum (biovar 2) in Patients With Non-gonococcal Urethritis Using Polymerase Chain Reaction-microtiter Plate Hybridization. Int J Urol. 2004;11(9):750-4. PubMed PMID: 15379939.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of Mycoplasma genitalium, Mycoplasma hominis, Ureaplasma parvum (biovar 1) and Ureaplasma urealyticum (biovar 2) in patients with non-gonococcal urethritis using polymerase chain reaction-microtiter plate hybridization. AU - Maeda,Shin-ichi, AU - Deguchi,Takashi, AU - Ishiko,Hiroaki, AU - Matsumoto,Tetsuro, AU - Naito,Seiji, AU - Kumon,Hiromi, AU - Tsukamoto,Taiji, AU - Onodera,Shouichi, AU - Kamidono,Sadao, PY - 2004/9/24/pubmed PY - 2005/1/14/medline PY - 2004/9/24/entrez SP - 750 EP - 4 JF - International journal of urology : official journal of the Japanese Urological Association JO - Int. J. Urol. VL - 11 IS - 9 N2 - AIM: To use polymerase chain reaction (PCR)-microtiter plate hybridization assays to detect Mycoplasma genitalium, Mycoplasma hominis, Ureaplasma parvum (biovar 1) and Ureaplasma urealyticum (biovar 2) in first-voided urine specimens from patients with non-gonococcal urethritis (NGU). METHODS: A total of 153 male patients with NGU, who visited one of 24 clinics in Japan, were recruited for this study. All were examined using PCR-microtiter plate hybridization assays for the presence of M. genitalium, M. hominis, U. parvum (biovar 1) and U. urealyticum (biovar 2) in first-voided urine specimens. They were also examined for the presence of Chlamydia trachomatis. RESULTS: Of these 153 patients, 73 (47.7%) were positive for C. trachomatis. Overall, the prevalence was 17.0% for M. genitalium, 16.3% for U. urealyticum (biovar 2), 7.8% for U. parvum (biovar 1) and 2.6% for M. hominis. In the 80 patients with non-chlamydial NGU, the prevalence of M. genitalium, U. urealyticum (biovar 2), U. parvum (biovar 1) and M. hominis was 23.8%, 18.8%, 8.8% and 2.6%, respectively. CONCLUSIONS: This study shows the prevalence of mycoplasmas and ureaplasmas in NGU in Japan. M. genitalium and U. urealyticum (biovar 2) might be pathogens of NGU and could be associated with persistent and recurrent urethritis. When patients with NGU are treated, such pathogens should be taken into account. This PCR-microtiter plate hybridization assay provides a useful method for diagnosing NGU caused by M. genitalium and U. urealyticum (biovar 2). SN - 0919-8172 UR - https://www.unboundmedicine.com/medline/citation/15379939/Detection_of_Mycoplasma_genitalium_Mycoplasma_hominis_Ureaplasma_parvum__biovar_1__and_Ureaplasma_urealyticum__biovar_2__in_patients_with_non_gonococcal_urethritis_using_polymerase_chain_reaction_microtiter_plate_hybridization_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0919-8172&date=2004&volume=11&issue=9&spage=750 DB - PRIME DP - Unbound Medicine ER -