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Determination of Delta9-tetrahydrocannabinol from rabbit plasma by gas chromatography-mass spectrometry using two ionization techniques.
J Chromatogr B Analyt Technol Biomed Life Sci. 2004 Oct 25; 810(2):283-90.JC

Abstract

The purpose of the study was to develop a gas chromatography-mass spectrometric (GC-MS) method for the identification and quantitation of Delta(9)-tetrahydrocannabinol (THC) in rabbit plasma. Two ionization techniques were utilized for GC-MS: electron impact ionization (EI) after i.v. administration and negative chemical ionization (NCI) after sublingual administration. THC was isolated from plasma by solid phase extraction and derivatized by either trimethylsilylation (EI) or trifuoroacetylation (NCI), with deuterated THC as an internal standard. The validity of analytical method was confirmed by investigating selectivity, limit of quantitation, linearity, accuracy, precision, recovery and stability of the analyte. The method proved to be selective, linear, accurate and precise over a range of 10-430 and 0.3-530 ng/ml of THC in plasma for EI and NCI, respectively. The extraction recovery was >81% for each concentration level studied, and the analyte was shown to be stable during storage and sample preparation. The method was applied successfully in analysing THC from rabbit plasma.

Authors+Show Affiliations

Department of Pharmaceutical Chemistry, University of Kuopio, P.O. Box 1627, FIN-70211 Kuopio, Finland. janne.mannila@uku.fiNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15380726

Citation

Mannila, Janne, et al. "Determination of Delta9-tetrahydrocannabinol From Rabbit Plasma By Gas Chromatography-mass Spectrometry Using Two Ionization Techniques." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 810, no. 2, 2004, pp. 283-90.
Mannila J, Lehtonen M, Järvinen T, et al. Determination of Delta9-tetrahydrocannabinol from rabbit plasma by gas chromatography-mass spectrometry using two ionization techniques. J Chromatogr B Analyt Technol Biomed Life Sci. 2004;810(2):283-90.
Mannila, J., Lehtonen, M., Järvinen, T., & Jarho, P. (2004). Determination of Delta9-tetrahydrocannabinol from rabbit plasma by gas chromatography-mass spectrometry using two ionization techniques. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 810(2), 283-90.
Mannila J, et al. Determination of Delta9-tetrahydrocannabinol From Rabbit Plasma By Gas Chromatography-mass Spectrometry Using Two Ionization Techniques. J Chromatogr B Analyt Technol Biomed Life Sci. 2004 Oct 25;810(2):283-90. PubMed PMID: 15380726.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of Delta9-tetrahydrocannabinol from rabbit plasma by gas chromatography-mass spectrometry using two ionization techniques. AU - Mannila,Janne, AU - Lehtonen,Marko, AU - Järvinen,Tomi, AU - Jarho,Pekka, PY - 2004/03/24/received PY - 2004/08/10/accepted PY - 2004/9/24/pubmed PY - 2005/2/26/medline PY - 2004/9/24/entrez SP - 283 EP - 90 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 810 IS - 2 N2 - The purpose of the study was to develop a gas chromatography-mass spectrometric (GC-MS) method for the identification and quantitation of Delta(9)-tetrahydrocannabinol (THC) in rabbit plasma. Two ionization techniques were utilized for GC-MS: electron impact ionization (EI) after i.v. administration and negative chemical ionization (NCI) after sublingual administration. THC was isolated from plasma by solid phase extraction and derivatized by either trimethylsilylation (EI) or trifuoroacetylation (NCI), with deuterated THC as an internal standard. The validity of analytical method was confirmed by investigating selectivity, limit of quantitation, linearity, accuracy, precision, recovery and stability of the analyte. The method proved to be selective, linear, accurate and precise over a range of 10-430 and 0.3-530 ng/ml of THC in plasma for EI and NCI, respectively. The extraction recovery was >81% for each concentration level studied, and the analyte was shown to be stable during storage and sample preparation. The method was applied successfully in analysing THC from rabbit plasma. SN - 1570-0232 UR - https://www.unboundmedicine.com/medline/citation/15380726/Determination_of_Delta9_tetrahydrocannabinol_from_rabbit_plasma_by_gas_chromatography_mass_spectrometry_using_two_ionization_techniques_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570-0232(04)00658-0 DB - PRIME DP - Unbound Medicine ER -