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Development and validation of a method for the quantitation of Delta9tetrahydrocannabinol in oral fluid by liquid chromatography electrospray-mass-spectrometry.
J Chromatogr B Analyt Technol Biomed Life Sci. 2004 Oct 25; 810(2):319-24.JC

Abstract

Analysis of Delta(9)tetrahydrocannabinol (Delta(9)THC) and its metabolites in biological samples is of great relevance for forensic purposes. In the case of oral fluid (OF), the analysis should determine Delta(9)THC, whereas in urine, it detects the inactive metabolite tetrahydrocannabinol carboxylic acid (THC-COOH). Most laboratories analyze Delta(9)THC in such samples using GC-MS methods, but these procedures are time-consuming and involve unavoidable previous extraction and derivatization. No data is yet available on the application of liquid chromatography-mass-spectrometry to detect Delta(9)THC in oral fluid. We report a validation method in which the Delta(9)THC is isolated from oral fluid by a simple liquid-liquid extraction with hexane and subsequently analyzed by liquid chromatography-mass-spectrometry. The method here reported for the determination of Delta(9)THC in oral fluid only requires 200 microl of sample and achieves limits of detection of 2 ng/ml, and has been used to analyze oral fluid samples collected from current drug users.

Authors+Show Affiliations

Forensic Toxicology Service, Institute of Legal Medicine, Faculty of Medicine, Universidad de Santiago de Compostela, S. Francisco s/n, 15786 Santiago De Compostela, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15380731

Citation

Concheiro, M, et al. "Development and Validation of a Method for the Quantitation of Delta9tetrahydrocannabinol in Oral Fluid By Liquid Chromatography Electrospray-mass-spectrometry." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 810, no. 2, 2004, pp. 319-24.
Concheiro M, de Castro A, Quintela O, et al. Development and validation of a method for the quantitation of Delta9tetrahydrocannabinol in oral fluid by liquid chromatography electrospray-mass-spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci. 2004;810(2):319-24.
Concheiro, M., de Castro, A., Quintela, O., Cruz, A., & López-Rivadulla, M. (2004). Development and validation of a method for the quantitation of Delta9tetrahydrocannabinol in oral fluid by liquid chromatography electrospray-mass-spectrometry. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 810(2), 319-24.
Concheiro M, et al. Development and Validation of a Method for the Quantitation of Delta9tetrahydrocannabinol in Oral Fluid By Liquid Chromatography Electrospray-mass-spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci. 2004 Oct 25;810(2):319-24. PubMed PMID: 15380731.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development and validation of a method for the quantitation of Delta9tetrahydrocannabinol in oral fluid by liquid chromatography electrospray-mass-spectrometry. AU - Concheiro,M, AU - de Castro,A, AU - Quintela,O, AU - Cruz,A, AU - López-Rivadulla,M, PY - 2004/06/15/received PY - 2004/08/09/accepted PY - 2004/9/24/pubmed PY - 2005/2/26/medline PY - 2004/9/24/entrez SP - 319 EP - 24 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 810 IS - 2 N2 - Analysis of Delta(9)tetrahydrocannabinol (Delta(9)THC) and its metabolites in biological samples is of great relevance for forensic purposes. In the case of oral fluid (OF), the analysis should determine Delta(9)THC, whereas in urine, it detects the inactive metabolite tetrahydrocannabinol carboxylic acid (THC-COOH). Most laboratories analyze Delta(9)THC in such samples using GC-MS methods, but these procedures are time-consuming and involve unavoidable previous extraction and derivatization. No data is yet available on the application of liquid chromatography-mass-spectrometry to detect Delta(9)THC in oral fluid. We report a validation method in which the Delta(9)THC is isolated from oral fluid by a simple liquid-liquid extraction with hexane and subsequently analyzed by liquid chromatography-mass-spectrometry. The method here reported for the determination of Delta(9)THC in oral fluid only requires 200 microl of sample and achieves limits of detection of 2 ng/ml, and has been used to analyze oral fluid samples collected from current drug users. SN - 1570-0232 UR - https://www.unboundmedicine.com/medline/citation/15380731/Development_and_validation_of_a_method_for_the_quantitation_of_Delta9tetrahydrocannabinol_in_oral_fluid_by_liquid_chromatography_electrospray_mass_spectrometry_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570-0232(04)00650-6 DB - PRIME DP - Unbound Medicine ER -