Tags

Type your tag names separated by a space and hit enter

Molecular cloning and comparative analysis of a y-type inactive HMW glutenin subunit gene from cultivated emmer wheat (Triticum dicoccum L.).
Hereditas 2004; 141(1):46-54H

Abstract

Cultivated emmer (Triticum dicoccum, 2n = 4x = 28, AABB) is closely related to bread wheat and possesses extensive allelic variations in high molecular weight glutenin subunit (HMW-GS) composition. These alleles may be an important genetic resource for wheat quality improvement. To isolate and clone HMW-GS genes from cultivated emmer, two pairs of allele-specific (AS) PCR primers were designed to amplify the coding sequence of y-type HMW-GS genes and their upstream sequences, respectively. The results showed that single bands of strong amplification were obtained through AS-PCR of genomic DNA from emmer. After cloning and sequencing the complete sequence of coding and 5'-flanking regions of a y-type subunit gene at Glu-A1 locus was obtained. Nucleotide and deduced amino acid sequences analysis showed that this gene possessed a similar structure as the previously reported Ay gene from common wheat, and is hence designated as Ay1d. The distinct feature of the Ay1d gene is that its coding region contains four stop codons and its upstream region has a 85-bp deletion in the same position of the Ay gene, which are probably responsible for the silencing of y-type subunit genes at Glu-A1 locus. Phylogenetic analysis of HMW glutenin subunit genes from different Triticum species and genomes were also carried out.

Authors+Show Affiliations

Key Laboratory of Genetics and Biotechnology, Department of Biology, Capital Normal University, Beijing, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15383071

Citation

Sun, Minmin, et al. "Molecular Cloning and Comparative Analysis of a Y-type Inactive HMW Glutenin Subunit Gene From Cultivated Emmer Wheat (Triticum Dicoccum L.)." Hereditas, vol. 141, no. 1, 2004, pp. 46-54.
Sun M, Yan Y, Jiang Y, et al. Molecular cloning and comparative analysis of a y-type inactive HMW glutenin subunit gene from cultivated emmer wheat (Triticum dicoccum L.). Hereditas. 2004;141(1):46-54.
Sun, M., Yan, Y., Jiang, Y., Xiao, Y., Hu, Y., Cai, M., ... Zeller, F. J. (2004). Molecular cloning and comparative analysis of a y-type inactive HMW glutenin subunit gene from cultivated emmer wheat (Triticum dicoccum L.). Hereditas, 141(1), pp. 46-54.
Sun M, et al. Molecular Cloning and Comparative Analysis of a Y-type Inactive HMW Glutenin Subunit Gene From Cultivated Emmer Wheat (Triticum Dicoccum L.). Hereditas. 2004;141(1):46-54. PubMed PMID: 15383071.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular cloning and comparative analysis of a y-type inactive HMW glutenin subunit gene from cultivated emmer wheat (Triticum dicoccum L.). AU - Sun,Minmin, AU - Yan,Yueming, AU - Jiang,Yi, AU - Xiao,Yinghua, AU - Hu,Yingkao, AU - Cai,Minhua, AU - Li,Yaxuan, AU - Hsam,Sai L K, AU - Zeller,Friedrich J, PY - 2004/9/24/pubmed PY - 2004/11/13/medline PY - 2004/9/24/entrez SP - 46 EP - 54 JF - Hereditas JO - Hereditas VL - 141 IS - 1 N2 - Cultivated emmer (Triticum dicoccum, 2n = 4x = 28, AABB) is closely related to bread wheat and possesses extensive allelic variations in high molecular weight glutenin subunit (HMW-GS) composition. These alleles may be an important genetic resource for wheat quality improvement. To isolate and clone HMW-GS genes from cultivated emmer, two pairs of allele-specific (AS) PCR primers were designed to amplify the coding sequence of y-type HMW-GS genes and their upstream sequences, respectively. The results showed that single bands of strong amplification were obtained through AS-PCR of genomic DNA from emmer. After cloning and sequencing the complete sequence of coding and 5'-flanking regions of a y-type subunit gene at Glu-A1 locus was obtained. Nucleotide and deduced amino acid sequences analysis showed that this gene possessed a similar structure as the previously reported Ay gene from common wheat, and is hence designated as Ay1d. The distinct feature of the Ay1d gene is that its coding region contains four stop codons and its upstream region has a 85-bp deletion in the same position of the Ay gene, which are probably responsible for the silencing of y-type subunit genes at Glu-A1 locus. Phylogenetic analysis of HMW glutenin subunit genes from different Triticum species and genomes were also carried out. SN - 0018-0661 UR - https://www.unboundmedicine.com/medline/citation/15383071/Molecular_cloning_and_comparative_analysis_of_a_y_type_inactive_HMW_glutenin_subunit_gene_from_cultivated_emmer_wheat__Triticum_dicoccum_L___ L2 - https://doi.org/10.1111/j.1601-5223.2004.01835.x DB - PRIME DP - Unbound Medicine ER -