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The epaxial-hypaxial subdivision of the avian somite.
Dev Biol. 2004 Oct 15; 274(2):348-69.DB

Abstract

In all jaw-bearing vertebrates, three-dimensional mobility relies on segregated, separately innervated epaxial and hypaxial skeletal muscles. In amniotes, these muscles form from the morphologically continuous dermomyotome and myotome, whose epaxial-hypaxial subdivision and hence the formation of distinct epaxial-hypaxial muscles is not understood. Here we show that En1 expression labels a central subdomain of the avian dermomyotome, medially abutting the expression domain of the lead-lateral or hypaxial marker Sim1. En1 expression is maintained when cells from the En1-positive dermomyotome enter the myotome and dermatome, thereby superimposing the En1-Sim1 expression boundary onto the developing musculature and dermis. En1 cells originate from the dorsomedial edge of the somite. Their development is under positive control by notochord and floor plate (Shh), dorsal neural tube (Wnt1) and surface ectoderm (Wnt1-like signalling activity) but negatively regulated by the lateral plate mesoderm (BMP4). This dependence on epaxial signals and suppression by hypaxial signals places En1 into the epaxial somitic programme. Consequently, the En1-Sim1 expression boundary marks the epaxial-hypaxial dermomyotomal or myotomal boundary. In cell aggregation assays, En1- and Sim1-expressing cells sort out, suggesting that the En1-Sim1 expression boundary may represent a true compartment boundary, foreshadowing the epaxial-hypaxial segregation of muscle.

Authors+Show Affiliations

Department of Craniofacial Development, King's College London, London Bridge, London SE1 9RT, UK.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15385164

Citation

Cheng, Louise, et al. "The Epaxial-hypaxial Subdivision of the Avian Somite." Developmental Biology, vol. 274, no. 2, 2004, pp. 348-69.
Cheng L, Alvares LE, Ahmed MU, et al. The epaxial-hypaxial subdivision of the avian somite. Dev Biol. 2004;274(2):348-69.
Cheng, L., Alvares, L. E., Ahmed, M. U., El-Hanfy, A. S., & Dietrich, S. (2004). The epaxial-hypaxial subdivision of the avian somite. Developmental Biology, 274(2), 348-69.
Cheng L, et al. The Epaxial-hypaxial Subdivision of the Avian Somite. Dev Biol. 2004 Oct 15;274(2):348-69. PubMed PMID: 15385164.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The epaxial-hypaxial subdivision of the avian somite. AU - Cheng,Louise, AU - Alvares,Lúcia E, AU - Ahmed,Mohi U, AU - El-Hanfy,Amira S, AU - Dietrich,Susanne, PY - 2003/02/10/received PY - 2004/07/06/revised PY - 2004/07/07/accepted PY - 2004/9/24/pubmed PY - 2004/12/16/medline PY - 2004/9/24/entrez SP - 348 EP - 69 JF - Developmental biology JO - Dev Biol VL - 274 IS - 2 N2 - In all jaw-bearing vertebrates, three-dimensional mobility relies on segregated, separately innervated epaxial and hypaxial skeletal muscles. In amniotes, these muscles form from the morphologically continuous dermomyotome and myotome, whose epaxial-hypaxial subdivision and hence the formation of distinct epaxial-hypaxial muscles is not understood. Here we show that En1 expression labels a central subdomain of the avian dermomyotome, medially abutting the expression domain of the lead-lateral or hypaxial marker Sim1. En1 expression is maintained when cells from the En1-positive dermomyotome enter the myotome and dermatome, thereby superimposing the En1-Sim1 expression boundary onto the developing musculature and dermis. En1 cells originate from the dorsomedial edge of the somite. Their development is under positive control by notochord and floor plate (Shh), dorsal neural tube (Wnt1) and surface ectoderm (Wnt1-like signalling activity) but negatively regulated by the lateral plate mesoderm (BMP4). This dependence on epaxial signals and suppression by hypaxial signals places En1 into the epaxial somitic programme. Consequently, the En1-Sim1 expression boundary marks the epaxial-hypaxial dermomyotomal or myotomal boundary. In cell aggregation assays, En1- and Sim1-expressing cells sort out, suggesting that the En1-Sim1 expression boundary may represent a true compartment boundary, foreshadowing the epaxial-hypaxial segregation of muscle. SN - 0012-1606 UR - https://www.unboundmedicine.com/medline/citation/15385164/The_epaxial_hypaxial_subdivision_of_the_avian_somite_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0012-1606(04)00495-6 DB - PRIME DP - Unbound Medicine ER -