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Hair analysis of nicotine and cotinine for evaluating tobacco smoke exposure by liquid chromatography-mass spectrometry.
Biomed Chromatogr. 2004 Nov; 18(9):655-61.BC

Abstract

A simple liquid chromatography-electrospray ionization mass spectrometry (LC-ESI-MS) method for the determination of nicotine and cotinine in human hair was established. In the procedure, a hair sample (10 mg) was washed with dichloromethane and digested in 2.5 M sodium hydroxide. The digest was extracted with dichloromethane and then 25 mM hydrochloric acid in methanol was added to the extract, to prevent loss of analytes. The solution was evaporated and redissolved in the mobile phase, methanol/10 mM ammonium acetate (30/70, v/v). A 20 microL aliquot of redissolved solution was subjected to analysis. Nicotine and cotinine in human hair were quantified by using deuterated analytes as internal standards. The quantification limits were 8 microg/L for nicotine and 0.9 microg/L for cotinine. The proposed method was applied to measure the concentrations of nicotine and cotinine in hair of smokers and non-smokers to evaluate their self-reported smoking and exposure to environmental tobacco smoke. In both cases, the method provided good selectivity, accuracy and precision.

Authors+Show Affiliations

Chetiyanukornkul T
Graduate School of Natural Science and Technology, Kanazawa University, Kakuma-machi, Kanazawa, Ishikawa 920-1192, Japan.
Toriba A
No affiliation info available
Kizu R
No affiliation info available
Kimura K
No affiliation info available
Hayakawa K
No affiliation info available

MeSH

Chromatography, LiquidCotinineHairHumansNicotineReference StandardsSmokeSpectrometry, Mass, Electrospray IonizationTobaccoTobacco Smoke Pollution

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15386502

Citation

Chetiyanukornkul, Thaneeya, et al. "Hair Analysis of Nicotine and Cotinine for Evaluating Tobacco Smoke Exposure By Liquid Chromatography-mass Spectrometry." Biomedical Chromatography : BMC, vol. 18, no. 9, 2004, pp. 655-61.
Chetiyanukornkul T, Toriba A, Kizu R, et al. Hair analysis of nicotine and cotinine for evaluating tobacco smoke exposure by liquid chromatography-mass spectrometry. Biomed Chromatogr. 2004;18(9):655-61.
Chetiyanukornkul, T., Toriba, A., Kizu, R., Kimura, K., & Hayakawa, K. (2004). Hair analysis of nicotine and cotinine for evaluating tobacco smoke exposure by liquid chromatography-mass spectrometry. Biomedical Chromatography : BMC, 18(9), 655-61.
Chetiyanukornkul T, et al. Hair Analysis of Nicotine and Cotinine for Evaluating Tobacco Smoke Exposure By Liquid Chromatography-mass Spectrometry. Biomed Chromatogr. 2004;18(9):655-61. PubMed PMID: 15386502.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Hair analysis of nicotine and cotinine for evaluating tobacco smoke exposure by liquid chromatography-mass spectrometry. AU - Chetiyanukornkul,Thaneeya, AU - Toriba,Akira, AU - Kizu,Ryoichi, AU - Kimura,Kazuko, AU - Hayakawa,Kazuichi, PY - 2004/9/24/pubmed PY - 2005/4/26/medline PY - 2004/9/24/entrez SP - 655 EP - 61 JF - Biomedical chromatography : BMC JO - Biomed Chromatogr VL - 18 IS - 9 N2 - A simple liquid chromatography-electrospray ionization mass spectrometry (LC-ESI-MS) method for the determination of nicotine and cotinine in human hair was established. In the procedure, a hair sample (10 mg) was washed with dichloromethane and digested in 2.5 M sodium hydroxide. The digest was extracted with dichloromethane and then 25 mM hydrochloric acid in methanol was added to the extract, to prevent loss of analytes. The solution was evaporated and redissolved in the mobile phase, methanol/10 mM ammonium acetate (30/70, v/v). A 20 microL aliquot of redissolved solution was subjected to analysis. Nicotine and cotinine in human hair were quantified by using deuterated analytes as internal standards. The quantification limits were 8 microg/L for nicotine and 0.9 microg/L for cotinine. The proposed method was applied to measure the concentrations of nicotine and cotinine in hair of smokers and non-smokers to evaluate their self-reported smoking and exposure to environmental tobacco smoke. In both cases, the method provided good selectivity, accuracy and precision. SN - 0269-3879 UR - https://www.unboundmedicine.com/medline/citation/15386502/Hair_analysis_of_nicotine_and_cotinine_for_evaluating_tobacco_smoke_exposure_by_liquid_chromatography_mass_spectrometry_ DB - PRIME DP - Unbound Medicine ER -