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Microemulsion electrokinetic chromatography with laser-induced fluorescence detection: as tested with amino acid derivatives.
Biomed Chromatogr. 2004 Oct; 18(8):600-7.BC

Abstract

Over a decade ago, microemulsion electrokinetic chromatography was introduced as a novel mode of capillary electrophoresis. However, there has not been publication on the combination of microemulsion electrokinetic chromatography with laser-induced fluorescence detection. In this paper, a preliminary method using microemulsion eletrokinetic chromatography combined with laser-induced fluorescence detection and second derivative electrophoregram was established as a sensitive and selective assay for separation and determination of nine amino acids after derivatization with 4-chloro-7-nitrobenzo-2-oxa-1, 3-diazol. The derivatization and separation conditions were optimized. In the investigated concentration ranges correlation coefficients were better than 0.995. The relative standard deviation (n = 5) of the migration times and peak heights were 0.56-0.76 and 2.21-7.15%, respectively. The detection limits (S/N = 3) were at a neaomolar level (0.32-2.20 nM). The method was applied for the analysis of compound amino acid injection and a Chinese traditional herbal medicine. The recoveries were 95.9-107.9%.

Authors+Show Affiliations

Department of chemistry, Lanzhou University, Lanzhou 730000, People's Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15386510

Citation

Jianping, Xie, et al. "Microemulsion Electrokinetic Chromatography With Laser-induced Fluorescence Detection: as Tested With Amino Acid Derivatives." Biomedical Chromatography : BMC, vol. 18, no. 8, 2004, pp. 600-7.
Jianping X, Jiyou Z, Huanxiang L, et al. Microemulsion electrokinetic chromatography with laser-induced fluorescence detection: as tested with amino acid derivatives. Biomed Chromatogr. 2004;18(8):600-7.
Jianping, X., Jiyou, Z., Huanxiang, L., Jiaqin, L., Jianniao, T., Xingguo, C., & Zhide, H. (2004). Microemulsion electrokinetic chromatography with laser-induced fluorescence detection: as tested with amino acid derivatives. Biomedical Chromatography : BMC, 18(8), 600-7.
Jianping X, et al. Microemulsion Electrokinetic Chromatography With Laser-induced Fluorescence Detection: as Tested With Amino Acid Derivatives. Biomed Chromatogr. 2004;18(8):600-7. PubMed PMID: 15386510.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Microemulsion electrokinetic chromatography with laser-induced fluorescence detection: as tested with amino acid derivatives. AU - Jianping,Xie, AU - Jiyou,Zhang, AU - Huanxiang,Liu, AU - Jiaqin,Liu, AU - Jianniao,Tian, AU - Xingguo,Chen, AU - Zhide,Hu, PY - 2004/9/24/pubmed PY - 2005/2/11/medline PY - 2004/9/24/entrez SP - 600 EP - 7 JF - Biomedical chromatography : BMC JO - Biomed Chromatogr VL - 18 IS - 8 N2 - Over a decade ago, microemulsion electrokinetic chromatography was introduced as a novel mode of capillary electrophoresis. However, there has not been publication on the combination of microemulsion electrokinetic chromatography with laser-induced fluorescence detection. In this paper, a preliminary method using microemulsion eletrokinetic chromatography combined with laser-induced fluorescence detection and second derivative electrophoregram was established as a sensitive and selective assay for separation and determination of nine amino acids after derivatization with 4-chloro-7-nitrobenzo-2-oxa-1, 3-diazol. The derivatization and separation conditions were optimized. In the investigated concentration ranges correlation coefficients were better than 0.995. The relative standard deviation (n = 5) of the migration times and peak heights were 0.56-0.76 and 2.21-7.15%, respectively. The detection limits (S/N = 3) were at a neaomolar level (0.32-2.20 nM). The method was applied for the analysis of compound amino acid injection and a Chinese traditional herbal medicine. The recoveries were 95.9-107.9%. SN - 0269-3879 UR - https://www.unboundmedicine.com/medline/citation/15386510/Microemulsion_electrokinetic_chromatography_with_laser_induced_fluorescence_detection:_as_tested_with_amino_acid_derivatives_ L2 - https://doi.org/10.1002/bmc.362 DB - PRIME DP - Unbound Medicine ER -