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Continuous capillary electrophoresis with flow injection and its application for determination of Ephedrine and Pseudo-ephedrine in Chinese medicinal preparations.
Biomed Chromatogr. 2004 Oct; 18(8):581-8.BC

Abstract

This article presents a new, simple and rapid continuous separation method by combination of flow injection with capillary electrophoresis designed for the analysis of basic traditional Chinese medicines. The device was produced using commercial capillary and components readily available in analytical laboratory. In double-T configuration, the designed horizontal separation channel was 25 microm i.d. x 146 mm length (an effective separation length of 93 mm) quartz capillary, with two vertical elicitation arms produced from 0.5 mm i.d. pump tubing. The capillary was embedded in a 40 x 20 x 3 mm organic glass base. Using the double-T configuration, continuous introduction of a series of samples was achieved. More than 3.00 resolution for ephedrine and pseudo-ephedrine were obtained using 100 mm borate buffer (pH 9.80) within 8 min in 25 microm separation channel with an electrical field strength of 137 V/cm (UV detection at 215 nm). The linear calibration range was 50-1500 microg/mL (ephedrine, r = 0.9982; pseudo-ephedrine, r = 0.9990) for both analytes. The limits of detection were 2.65 micro g/mL for ephedrine and 2.92 microg/mL for pseudo-ephedrine. In this device, the contents of ephedrine and pseudo-ephedrine in five Chinese medicinal preparations were determined with RSDs (n = 5) in range 1.16-4.51% and recoveries in range 90.4-114.6%.

Authors+Show Affiliations

Department of Chemistry, Lanzhou University, Lanzhou 730000, People's Republic of China.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15386512

Citation

Pan, Zhongwei, et al. "Continuous Capillary Electrophoresis With Flow Injection and Its Application for Determination of Ephedrine and Pseudo-ephedrine in Chinese Medicinal Preparations." Biomedical Chromatography : BMC, vol. 18, no. 8, 2004, pp. 581-8.
Pan Z, Chen X, Hu Z. Continuous capillary electrophoresis with flow injection and its application for determination of Ephedrine and Pseudo-ephedrine in Chinese medicinal preparations. Biomed Chromatogr. 2004;18(8):581-8.
Pan, Z., Chen, X., & Hu, Z. (2004). Continuous capillary electrophoresis with flow injection and its application for determination of Ephedrine and Pseudo-ephedrine in Chinese medicinal preparations. Biomedical Chromatography : BMC, 18(8), 581-8.
Pan Z, Chen X, Hu Z. Continuous Capillary Electrophoresis With Flow Injection and Its Application for Determination of Ephedrine and Pseudo-ephedrine in Chinese Medicinal Preparations. Biomed Chromatogr. 2004;18(8):581-8. PubMed PMID: 15386512.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Continuous capillary electrophoresis with flow injection and its application for determination of Ephedrine and Pseudo-ephedrine in Chinese medicinal preparations. AU - Pan,Zhongwei, AU - Chen,Xingguo, AU - Hu,Zhide, PY - 2004/9/24/pubmed PY - 2005/2/11/medline PY - 2004/9/24/entrez SP - 581 EP - 8 JF - Biomedical chromatography : BMC JO - Biomed Chromatogr VL - 18 IS - 8 N2 - This article presents a new, simple and rapid continuous separation method by combination of flow injection with capillary electrophoresis designed for the analysis of basic traditional Chinese medicines. The device was produced using commercial capillary and components readily available in analytical laboratory. In double-T configuration, the designed horizontal separation channel was 25 microm i.d. x 146 mm length (an effective separation length of 93 mm) quartz capillary, with two vertical elicitation arms produced from 0.5 mm i.d. pump tubing. The capillary was embedded in a 40 x 20 x 3 mm organic glass base. Using the double-T configuration, continuous introduction of a series of samples was achieved. More than 3.00 resolution for ephedrine and pseudo-ephedrine were obtained using 100 mm borate buffer (pH 9.80) within 8 min in 25 microm separation channel with an electrical field strength of 137 V/cm (UV detection at 215 nm). The linear calibration range was 50-1500 microg/mL (ephedrine, r = 0.9982; pseudo-ephedrine, r = 0.9990) for both analytes. The limits of detection were 2.65 micro g/mL for ephedrine and 2.92 microg/mL for pseudo-ephedrine. In this device, the contents of ephedrine and pseudo-ephedrine in five Chinese medicinal preparations were determined with RSDs (n = 5) in range 1.16-4.51% and recoveries in range 90.4-114.6%. SN - 0269-3879 UR - https://www.unboundmedicine.com/medline/citation/15386512/Continuous_capillary_electrophoresis_with_flow_injection_and_its_application_for_determination_of_Ephedrine_and_Pseudo_ephedrine_in_Chinese_medicinal_preparations_ L2 - https://doi.org/10.1002/bmc.360 DB - PRIME DP - Unbound Medicine ER -