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Rapid and sensitive determination of ephedrine and pseudoephedrine by micellar electrokinetic chromatography with on-line regenerating covalent coating.
Biomed Chromatogr. 2005 Jan; 19(1):9-14.BC

Abstract

A rapid, sensitive and reproducible micellar electrokinetic chromatographic method using hexamethyldisilazane as on-line regenerating covalent coating was developed for the quantification of ephedrine (E) and pseudoephedrine (PE). E and PE were derivatized with 4-chloro-7-nitrobenzo-2-oxa-1,3-diazol for laser-induced fluorescence detection. The on-line regenerating covalent coating formed a combinative double coating with the subsequently produced dynamic SDS coating. The total coating can be easily removed and conveniently regenerated on-line. The simple coating procedure was described. By a series of optimization, a running buffer of 20 mm Na(2)B(4)O(7) + 16 mm SDS was applied for the separation of the derivatives. Linear relationships for E and PE were obtained in the range of 0.044-6.60 microg mL(-1) (correlation coefficients: 0.9975 for E, 0.9981 for PE), and the detection limits for E and PE were 1.71 and 0.67 ng mL(-1), respectively. The separation speed, the reproducibility and the sensitivity were much improved over those of other capillary electrophoresis methods more recently reported. The method was applied to the analysis of the two alkaloids in traditional herbal preparations with recoveries in the range 92.8-104.8%.

Authors+Show Affiliations

Department of Chemistry, Lanzhou University, Lanzhou 730000, People's Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15386565

Citation

Jianping, Xie, et al. "Rapid and Sensitive Determination of Ephedrine and Pseudoephedrine By Micellar Electrokinetic Chromatography With On-line Regenerating Covalent Coating." Biomedical Chromatography : BMC, vol. 19, no. 1, 2005, pp. 9-14.
Jianping X, Jiyou Z, Jiaqin L, et al. Rapid and sensitive determination of ephedrine and pseudoephedrine by micellar electrokinetic chromatography with on-line regenerating covalent coating. Biomed Chromatogr. 2005;19(1):9-14.
Jianping, X., Jiyou, Z., Jiaqin, L., Jianniao, T., Xingguo, C., & Zhide, H. (2005). Rapid and sensitive determination of ephedrine and pseudoephedrine by micellar electrokinetic chromatography with on-line regenerating covalent coating. Biomedical Chromatography : BMC, 19(1), 9-14.
Jianping X, et al. Rapid and Sensitive Determination of Ephedrine and Pseudoephedrine By Micellar Electrokinetic Chromatography With On-line Regenerating Covalent Coating. Biomed Chromatogr. 2005;19(1):9-14. PubMed PMID: 15386565.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rapid and sensitive determination of ephedrine and pseudoephedrine by micellar electrokinetic chromatography with on-line regenerating covalent coating. AU - Jianping,Xie, AU - Jiyou,Zhang, AU - Jiaqin,Liu, AU - Jianniao,Tian, AU - Xingguo,Chen, AU - Zhide,Hu, PY - 2004/9/24/pubmed PY - 2005/7/13/medline PY - 2004/9/24/entrez SP - 9 EP - 14 JF - Biomedical chromatography : BMC JO - Biomed Chromatogr VL - 19 IS - 1 N2 - A rapid, sensitive and reproducible micellar electrokinetic chromatographic method using hexamethyldisilazane as on-line regenerating covalent coating was developed for the quantification of ephedrine (E) and pseudoephedrine (PE). E and PE were derivatized with 4-chloro-7-nitrobenzo-2-oxa-1,3-diazol for laser-induced fluorescence detection. The on-line regenerating covalent coating formed a combinative double coating with the subsequently produced dynamic SDS coating. The total coating can be easily removed and conveniently regenerated on-line. The simple coating procedure was described. By a series of optimization, a running buffer of 20 mm Na(2)B(4)O(7) + 16 mm SDS was applied for the separation of the derivatives. Linear relationships for E and PE were obtained in the range of 0.044-6.60 microg mL(-1) (correlation coefficients: 0.9975 for E, 0.9981 for PE), and the detection limits for E and PE were 1.71 and 0.67 ng mL(-1), respectively. The separation speed, the reproducibility and the sensitivity were much improved over those of other capillary electrophoresis methods more recently reported. The method was applied to the analysis of the two alkaloids in traditional herbal preparations with recoveries in the range 92.8-104.8%. SN - 0269-3879 UR - https://www.unboundmedicine.com/medline/citation/15386565/Rapid_and_sensitive_determination_of_ephedrine_and_pseudoephedrine_by_micellar_electrokinetic_chromatography_with_on_line_regenerating_covalent_coating_ L2 - https://doi.org/10.1002/bmc.407 DB - PRIME DP - Unbound Medicine ER -