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Characterization of starch synthase I and II expressed in early developing seeds of kidney bean (Phaseolus vulgaris L.).
Biosci Biotechnol Biochem. 2004 Sep; 68(9):1949-60.BB

Abstract

Plant starch synthase (SS) contributes to the elongation of glucan chains during starch biosynthesis and hence plays an essential role in determining the fine structure of amylopectin. To elucidate the role of SS activity in the formation of amylopectin in kidney bean (Phaseolus vulgaris L.), a study was undertaken to isolate cDNA clones for SS and to characterize the enzymatic properties of the coded recombinant enzymes. Two SS cDNAs, designated pvss1 and pvss21, which were isolated from early developing seeds, encoded SSI and SSII (designated PvSSI and PvSSII-1) that displayed significant identity (more than 65%) with other SSI and SSII members, respectively. RNA gel blot analysis indicated that both transcripts accumulate in leaves and developing seeds at the early stage. Immunoblot analysis with antisera raised against both recombinant proteins (rPvSSI and rPvSSII-1) showed that the accumulation of both proteins parallels the gene expression profiles, although both were detectable only in starch-granule fractions. Recombinant enzymes expressed by Escherichia coli cells showed distinct chain-length specificities for the extension of glucan chains. Our results suggest that these SS isozymes for synthesis of transitory starch are also responsible for synthesis of storage starch in early developing seeds of kidney bean.

Authors+Show Affiliations

Senoura T
Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Sapporo, Japan.
Isono N
No affiliation info available
Yoshikawa M
No affiliation info available
Asao A
No affiliation info available
Hamada S
No affiliation info available
Watanabe K
No affiliation info available
Ito H
No affiliation info available
Matsui H
No affiliation info available

MeSH

Amino Acid SequenceCloning, MolecularGene Expression RegulationGene Expression Regulation, DevelopmentalGene Expression Regulation, EnzymologicGene Expression Regulation, PlantMolecular Sequence DataPhaseolusPhylogenyPlant ProteinsRNA, PlantSeedsSequence AlignmentStarchStarch Synthase

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15388972

Citation

Senoura, Takeshi, et al. "Characterization of Starch Synthase I and II Expressed in Early Developing Seeds of Kidney Bean (Phaseolus Vulgaris L.)." Bioscience, Biotechnology, and Biochemistry, vol. 68, no. 9, 2004, pp. 1949-60.
Senoura T, Isono N, Yoshikawa M, et al. Characterization of starch synthase I and II expressed in early developing seeds of kidney bean (Phaseolus vulgaris L.). Biosci Biotechnol Biochem. 2004;68(9):1949-60.
Senoura, T., Isono, N., Yoshikawa, M., Asao, A., Hamada, S., Watanabe, K., Ito, H., & Matsui, H. (2004). Characterization of starch synthase I and II expressed in early developing seeds of kidney bean (Phaseolus vulgaris L.). Bioscience, Biotechnology, and Biochemistry, 68(9), 1949-60.
Senoura T, et al. Characterization of Starch Synthase I and II Expressed in Early Developing Seeds of Kidney Bean (Phaseolus Vulgaris L.). Biosci Biotechnol Biochem. 2004;68(9):1949-60. PubMed PMID: 15388972.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of starch synthase I and II expressed in early developing seeds of kidney bean (Phaseolus vulgaris L.). AU - Senoura,Takeshi, AU - Isono,Naoto, AU - Yoshikawa,Motoyo, AU - Asao,Ayako, AU - Hamada,Shigeki, AU - Watanabe,Kenji, AU - Ito,Hiroyuki, AU - Matsui,Hirokazu, PY - 2004/9/25/pubmed PY - 2005/4/15/medline PY - 2004/9/25/entrez SP - 1949 EP - 60 JF - Bioscience, biotechnology, and biochemistry JO - Biosci Biotechnol Biochem VL - 68 IS - 9 N2 - Plant starch synthase (SS) contributes to the elongation of glucan chains during starch biosynthesis and hence plays an essential role in determining the fine structure of amylopectin. To elucidate the role of SS activity in the formation of amylopectin in kidney bean (Phaseolus vulgaris L.), a study was undertaken to isolate cDNA clones for SS and to characterize the enzymatic properties of the coded recombinant enzymes. Two SS cDNAs, designated pvss1 and pvss21, which were isolated from early developing seeds, encoded SSI and SSII (designated PvSSI and PvSSII-1) that displayed significant identity (more than 65%) with other SSI and SSII members, respectively. RNA gel blot analysis indicated that both transcripts accumulate in leaves and developing seeds at the early stage. Immunoblot analysis with antisera raised against both recombinant proteins (rPvSSI and rPvSSII-1) showed that the accumulation of both proteins parallels the gene expression profiles, although both were detectable only in starch-granule fractions. Recombinant enzymes expressed by Escherichia coli cells showed distinct chain-length specificities for the extension of glucan chains. Our results suggest that these SS isozymes for synthesis of transitory starch are also responsible for synthesis of storage starch in early developing seeds of kidney bean. SN - 0916-8451 UR - https://www.unboundmedicine.com/medline/citation/15388972/Characterization_of_starch_synthase_I_and_II_expressed_in_early_developing_seeds_of_kidney_bean__Phaseolus_vulgaris_L___ DB - PRIME DP - Unbound Medicine ER -