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Neurogranin is expressed by principal cells but not interneurons in the rodent and monkey neocortex and hippocampus.
J Comp Neurol 2004; 479(1):30-42JC

Abstract

As a substrate of protein kinase C (PKC), neurogranin (NG) is involved in the regulation of calcium signaling and activity-dependent plasticity. Recently, we have shown that, in the rodent cerebellum, NG is exclusively expressed by gamma-aminobutyric acidergic Golgi cells, whereas, in the monkey cerebellum, brush cells were the only neuronal population expressing NG (Singec et al. [2003] J. Comp. Neurol. 459:278-289). In the present study, we analyzed the neocortical and hippocampal expression patterns of NG in adult mouse (C57Bl/6), rat (Wistar), and monkey (Cercopithecus aetiops). By using immunocytochemistry and nonradioactive in situ hybridization, we demonstrate strong NG expression by principal cells in different neocortical layers and in the hippocampus by granule cells of the dentate gyrus and pyramidal neurons of CA1-CA3. In contrast, double-labeling experiments in rodents revealed that neocortical and hippocampal interneurons expressing glutamate decarboxylase 67 (GAD67) were consistently devoid of NG. In addition, by using antibodies against parvalbumin, calbindin, and calretinin, we could demonstrate the absence of NG in interneurons of monkey frontal cortex and hippocampus. Together these findings corroborate the idea of different calcium signaling pathways in excitatory and inhibitory cells that may contribute to different modes of synaptic plasticity in these neurons.

Authors+Show Affiliations

Institute of Anatomy and Cell Biology, University of Freiburg, D-79104 Freiburg, Germany. singeci@ninds.nih.govNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15389613

Citation

Singec, Ilyas, et al. "Neurogranin Is Expressed By Principal Cells but Not Interneurons in the Rodent and Monkey Neocortex and Hippocampus." The Journal of Comparative Neurology, vol. 479, no. 1, 2004, pp. 30-42.
Singec I, Knoth R, Ditter M, et al. Neurogranin is expressed by principal cells but not interneurons in the rodent and monkey neocortex and hippocampus. J Comp Neurol. 2004;479(1):30-42.
Singec, I., Knoth, R., Ditter, M., Volk, B., & Frotscher, M. (2004). Neurogranin is expressed by principal cells but not interneurons in the rodent and monkey neocortex and hippocampus. The Journal of Comparative Neurology, 479(1), pp. 30-42.
Singec I, et al. Neurogranin Is Expressed By Principal Cells but Not Interneurons in the Rodent and Monkey Neocortex and Hippocampus. J Comp Neurol. 2004 Nov 1;479(1):30-42. PubMed PMID: 15389613.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Neurogranin is expressed by principal cells but not interneurons in the rodent and monkey neocortex and hippocampus. AU - Singec,Ilyas, AU - Knoth,Rolf, AU - Ditter,Margarethe, AU - Volk,Benedikt, AU - Frotscher,Michael, PY - 2004/9/25/pubmed PY - 2004/12/22/medline PY - 2004/9/25/entrez SP - 30 EP - 42 JF - The Journal of comparative neurology JO - J. Comp. Neurol. VL - 479 IS - 1 N2 - As a substrate of protein kinase C (PKC), neurogranin (NG) is involved in the regulation of calcium signaling and activity-dependent plasticity. Recently, we have shown that, in the rodent cerebellum, NG is exclusively expressed by gamma-aminobutyric acidergic Golgi cells, whereas, in the monkey cerebellum, brush cells were the only neuronal population expressing NG (Singec et al. [2003] J. Comp. Neurol. 459:278-289). In the present study, we analyzed the neocortical and hippocampal expression patterns of NG in adult mouse (C57Bl/6), rat (Wistar), and monkey (Cercopithecus aetiops). By using immunocytochemistry and nonradioactive in situ hybridization, we demonstrate strong NG expression by principal cells in different neocortical layers and in the hippocampus by granule cells of the dentate gyrus and pyramidal neurons of CA1-CA3. In contrast, double-labeling experiments in rodents revealed that neocortical and hippocampal interneurons expressing glutamate decarboxylase 67 (GAD67) were consistently devoid of NG. In addition, by using antibodies against parvalbumin, calbindin, and calretinin, we could demonstrate the absence of NG in interneurons of monkey frontal cortex and hippocampus. Together these findings corroborate the idea of different calcium signaling pathways in excitatory and inhibitory cells that may contribute to different modes of synaptic plasticity in these neurons. SN - 0021-9967 UR - https://www.unboundmedicine.com/medline/citation/15389613/Neurogranin_is_expressed_by_principal_cells_but_not_interneurons_in_the_rodent_and_monkey_neocortex_and_hippocampus_ L2 - https://doi.org/10.1002/cne.20302 DB - PRIME DP - Unbound Medicine ER -