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Altered hippocampal short-term plasticity and associative memory in synaptotagmin IV (-/-) mice.
Hippocampus 2004; 14(8):964-74H

Abstract

Synaptotagmin IV (Syt IV) is an activity-inducible, secretory vesicle protein that is thought to function as an inhibitor of neurotransmitter release (Littleton et al. Nature 400:757-760, 1999). To test this hypothesis in neurons of the mammalian CNS, we measured field excitatory postsynaptic potentials (fEPSPs) in hippocampal slice preparations from Syt IV (-/-) mice. At Schaffer collateral synapses, the basal properties of neurotransmission are largely normal. However, two forms of short-term plasticity, paired-pulse facilitation (PPF) and post-tetanic potentiation (PTP), are significantly enhanced in area CA1 of Syt IV (-/-) slices. Similarly, the early stages of long-term potentiation (LTP) are also enhanced at these synapses. Consistent with the low levels of Syt IV observed in dentate granule cells, the mossy fiber synapses in Syt IV (-/-) slices display largely normal PPF and LTP. In addition, we find that Syt IV (-/-) mice have deficits in the associative passive avoidance memory paradigm, but are normal in the novel object recognition paradigm. The synaptic architecture and connectivity of Syt IV (-/-) brains is indistinguishable from wild-type mice as indicated by immunohistochemical analysis. These results suggest Syt IV is a presynaptic negative regulator of short-term plasticity in area CA1 of the hippocampus and is required for some, but not all, forms of hippocampus-dependent memory.

Authors+Show Affiliations

Department of Pharmacology, Box 357750, Health Sciences Building, University of Washington, Seattle, Washington 98195, USA.

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

15390175

Citation

Ferguson, Gregory D., et al. "Altered Hippocampal Short-term Plasticity and Associative Memory in Synaptotagmin IV (-/-) Mice." Hippocampus, vol. 14, no. 8, 2004, pp. 964-74.
Ferguson GD, Wang H, Herschman HR, et al. Altered hippocampal short-term plasticity and associative memory in synaptotagmin IV (-/-) mice. Hippocampus. 2004;14(8):964-74.
Ferguson, G. D., Wang, H., Herschman, H. R., & Storm, D. R. (2004). Altered hippocampal short-term plasticity and associative memory in synaptotagmin IV (-/-) mice. Hippocampus, 14(8), pp. 964-74.
Ferguson GD, et al. Altered Hippocampal Short-term Plasticity and Associative Memory in Synaptotagmin IV (-/-) Mice. Hippocampus. 2004;14(8):964-74. PubMed PMID: 15390175.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Altered hippocampal short-term plasticity and associative memory in synaptotagmin IV (-/-) mice. AU - Ferguson,Gregory D, AU - Wang,Hongbing, AU - Herschman,Harvey R, AU - Storm,Daniel R, PY - 2004/9/25/pubmed PY - 2005/3/3/medline PY - 2004/9/25/entrez SP - 964 EP - 74 JF - Hippocampus JO - Hippocampus VL - 14 IS - 8 N2 - Synaptotagmin IV (Syt IV) is an activity-inducible, secretory vesicle protein that is thought to function as an inhibitor of neurotransmitter release (Littleton et al. Nature 400:757-760, 1999). To test this hypothesis in neurons of the mammalian CNS, we measured field excitatory postsynaptic potentials (fEPSPs) in hippocampal slice preparations from Syt IV (-/-) mice. At Schaffer collateral synapses, the basal properties of neurotransmission are largely normal. However, two forms of short-term plasticity, paired-pulse facilitation (PPF) and post-tetanic potentiation (PTP), are significantly enhanced in area CA1 of Syt IV (-/-) slices. Similarly, the early stages of long-term potentiation (LTP) are also enhanced at these synapses. Consistent with the low levels of Syt IV observed in dentate granule cells, the mossy fiber synapses in Syt IV (-/-) slices display largely normal PPF and LTP. In addition, we find that Syt IV (-/-) mice have deficits in the associative passive avoidance memory paradigm, but are normal in the novel object recognition paradigm. The synaptic architecture and connectivity of Syt IV (-/-) brains is indistinguishable from wild-type mice as indicated by immunohistochemical analysis. These results suggest Syt IV is a presynaptic negative regulator of short-term plasticity in area CA1 of the hippocampus and is required for some, but not all, forms of hippocampus-dependent memory. SN - 1050-9631 UR - https://www.unboundmedicine.com/medline/citation/15390175/Altered_hippocampal_short_term_plasticity_and_associative_memory_in_synaptotagmin_IV___/___mice_ L2 - https://doi.org/10.1002/hipo.20013 DB - PRIME DP - Unbound Medicine ER -