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Determination and evaluation of the sequence and textural effects of the puroindoline a and puroindoline b genes in a population of synthetic hexaploid wheat.
Theor Appl Genet. 2004 Nov; 109(8):1597-603.TA

Abstract

Aegilops tauschii (2 n=2 x=14, DD) is a rich source of genetic variability for hexaploid wheat (Triticum aestivum, 2 n=6 x=42, AABBDD) improvement. This variability can be accessed through utilizing synthetic hexaploid wheat lines, which contain genomes from Ae. tauschii and T. turgidum (2 n=4 x=28, AABB). Numerous desirable characteristics can and have been introgressed into common hexaploid wheat with this germplasm. In this work, the genetic variability in the two puroindoline genes (a and b) contained on the D genome, and the relationship that sequence polymorphisms in these genes have on endosperm texture among a population of 75 CIMMYT synthetic hexaploid accessions is described. Kernel texture was evaluated using the single kernel characterization system (SKCS). Kernel texture differed significantly (P<or=0.0001) among the synthetic hexaploid accessions (range 2.6-40.9) and the parent types, durum or Ae. tauschii. The interaction term between parent types was also a significant effect (P<or=0.0001). In addition to the 'wild-type' protein sequences of the puroindoline genes (those present in 'Chinese Spring' and all other soft wheats), three other translated sequences were identified in puroindoline a and two others in puroindoline b. These protein sequences were associated with significantly (P<or=0.0001) softer endosperm textures than the wild-type protein sequences. As the softer alleles are expressed in a hexaploid background, they are immediately available to wheat breeding programs.

Authors+Show Affiliations

Gedye KR
Department of Food Science and Human Nutrition, Washington State University, Pullman, WA 99164-6376, USA.
Morris CF
No affiliation info available
Bettge AD
No affiliation info available

MeSH

AgricultureAmino Acid SequenceAnalysis of VarianceBase SequenceCrops, AgriculturalGene Transfer TechniquesHybridization, GeneticMolecular Sequence DataPlant ProteinsPolyploidySeedsSequence AlignmentSequence Analysis, DNATriticum

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

15448897

Citation

Gedye, K R., et al. "Determination and Evaluation of the Sequence and Textural Effects of the Puroindoline a and Puroindoline B Genes in a Population of Synthetic Hexaploid Wheat." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 109, no. 8, 2004, pp. 1597-603.
Gedye KR, Morris CF, Bettge AD. Determination and evaluation of the sequence and textural effects of the puroindoline a and puroindoline b genes in a population of synthetic hexaploid wheat. Theor Appl Genet. 2004;109(8):1597-603.
Gedye, K. R., Morris, C. F., & Bettge, A. D. (2004). Determination and evaluation of the sequence and textural effects of the puroindoline a and puroindoline b genes in a population of synthetic hexaploid wheat. TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 109(8), 1597-603.
Gedye KR, Morris CF, Bettge AD. Determination and Evaluation of the Sequence and Textural Effects of the Puroindoline a and Puroindoline B Genes in a Population of Synthetic Hexaploid Wheat. Theor Appl Genet. 2004;109(8):1597-603. PubMed PMID: 15448897.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination and evaluation of the sequence and textural effects of the puroindoline a and puroindoline b genes in a population of synthetic hexaploid wheat. AU - Gedye,K R, AU - Morris,C F, AU - Bettge,A D, Y1 - 2004/09/22/ PY - 2004/05/16/received PY - 2004/08/04/accepted PY - 2004/9/28/pubmed PY - 2005/2/25/medline PY - 2004/9/28/entrez SP - 1597 EP - 603 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor Appl Genet VL - 109 IS - 8 N2 - Aegilops tauschii (2 n=2 x=14, DD) is a rich source of genetic variability for hexaploid wheat (Triticum aestivum, 2 n=6 x=42, AABBDD) improvement. This variability can be accessed through utilizing synthetic hexaploid wheat lines, which contain genomes from Ae. tauschii and T. turgidum (2 n=4 x=28, AABB). Numerous desirable characteristics can and have been introgressed into common hexaploid wheat with this germplasm. In this work, the genetic variability in the two puroindoline genes (a and b) contained on the D genome, and the relationship that sequence polymorphisms in these genes have on endosperm texture among a population of 75 CIMMYT synthetic hexaploid accessions is described. Kernel texture was evaluated using the single kernel characterization system (SKCS). Kernel texture differed significantly (P<or=0.0001) among the synthetic hexaploid accessions (range 2.6-40.9) and the parent types, durum or Ae. tauschii. The interaction term between parent types was also a significant effect (P<or=0.0001). In addition to the 'wild-type' protein sequences of the puroindoline genes (those present in 'Chinese Spring' and all other soft wheats), three other translated sequences were identified in puroindoline a and two others in puroindoline b. These protein sequences were associated with significantly (P<or=0.0001) softer endosperm textures than the wild-type protein sequences. As the softer alleles are expressed in a hexaploid background, they are immediately available to wheat breeding programs. SN - 0040-5752 UR - https://www.unboundmedicine.com/medline/citation/15448897/Determination_and_evaluation_of_the_sequence_and_textural_effects_of_the_puroindoline_a_and_puroindoline_b_genes_in_a_population_of_synthetic_hexaploid_wheat_ DB - PRIME DP - Unbound Medicine ER -