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Association of matrix metalloproteinase-9 in eosinophilic meningitis of BALB/c mice caused by Angiostrongylus cantonensis.
Parasitol Res. 2004 Nov; 94(5):321-8.PR

Abstract

Induction of gelatinase in eosinophilic meningitis of BALB/c-strain mice was caused by Angiostrongylus cantonensis. Time-course studies showed that the molecular weight of 94-kDa gelatinase was detected at day 10 post-inoculation (PI), and reached a high intensity from days 15 to 25 PI. The 94-kDa gelatinase activity was clearly inhibited by EDTA and 1,10-phenanthroline, but not by leupeptin and phenylmethanesulphonyl fluoride. When immunoblots were performed using specific antiserums against the 94-kDa gelatinase B (matrix metalloproteinase-9; MMP-9) with cerebrospinal fluid (CSF), the 94-kDa immunopositive band was MMP-9. Immunohistochemistry studies demonstrated MMP-9 localisation within eosinophils and macrophages. The increased MMP-9 activity was closely associated with the rapid rise of CSF eosinophils, and the inflammatory reaction of the subarachnoid space. In contrast to changes in MMP-9, MMP-2 activity was constitutive and unaffected in this parasitic meningitis. These results show that MMP-9 was associated with eosinophilic meningitis, and that the enzyme may be a useful marker for angiostrongyliasis meningitis.

Authors+Show Affiliations

Department of Parasitology, Chung Shan Medical University, 402 Taichung, Taiwan.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15449178

Citation

Lee, H H., et al. "Association of Matrix Metalloproteinase-9 in Eosinophilic Meningitis of BALB/c Mice Caused By Angiostrongylus Cantonensis." Parasitology Research, vol. 94, no. 5, 2004, pp. 321-8.
Lee HH, Chou HL, Chen KM, et al. Association of matrix metalloproteinase-9 in eosinophilic meningitis of BALB/c mice caused by Angiostrongylus cantonensis. Parasitol Res. 2004;94(5):321-8.
Lee, H. H., Chou, H. L., Chen, K. M., & Lai, S. C. (2004). Association of matrix metalloproteinase-9 in eosinophilic meningitis of BALB/c mice caused by Angiostrongylus cantonensis. Parasitology Research, 94(5), 321-8.
Lee HH, et al. Association of Matrix Metalloproteinase-9 in Eosinophilic Meningitis of BALB/c Mice Caused By Angiostrongylus Cantonensis. Parasitol Res. 2004;94(5):321-8. PubMed PMID: 15449178.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Association of matrix metalloproteinase-9 in eosinophilic meningitis of BALB/c mice caused by Angiostrongylus cantonensis. AU - Lee,H H, AU - Chou,H L, AU - Chen,K M, AU - Lai,S C, Y1 - 2004/09/21/ PY - 2004/04/30/received PY - 2004/07/15/accepted PY - 2004/9/28/pubmed PY - 2005/1/26/medline PY - 2004/9/28/entrez SP - 321 EP - 8 JF - Parasitology research JO - Parasitol Res VL - 94 IS - 5 N2 - Induction of gelatinase in eosinophilic meningitis of BALB/c-strain mice was caused by Angiostrongylus cantonensis. Time-course studies showed that the molecular weight of 94-kDa gelatinase was detected at day 10 post-inoculation (PI), and reached a high intensity from days 15 to 25 PI. The 94-kDa gelatinase activity was clearly inhibited by EDTA and 1,10-phenanthroline, but not by leupeptin and phenylmethanesulphonyl fluoride. When immunoblots were performed using specific antiserums against the 94-kDa gelatinase B (matrix metalloproteinase-9; MMP-9) with cerebrospinal fluid (CSF), the 94-kDa immunopositive band was MMP-9. Immunohistochemistry studies demonstrated MMP-9 localisation within eosinophils and macrophages. The increased MMP-9 activity was closely associated with the rapid rise of CSF eosinophils, and the inflammatory reaction of the subarachnoid space. In contrast to changes in MMP-9, MMP-2 activity was constitutive and unaffected in this parasitic meningitis. These results show that MMP-9 was associated with eosinophilic meningitis, and that the enzyme may be a useful marker for angiostrongyliasis meningitis. SN - 0932-0113 UR - https://www.unboundmedicine.com/medline/citation/15449178/Association_of_matrix_metalloproteinase_9_in_eosinophilic_meningitis_of_BALB/c_mice_caused_by_Angiostrongylus_cantonensis_ L2 - https://dx.doi.org/10.1007/s00436-004-1196-3 DB - PRIME DP - Unbound Medicine ER -