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Osmoregulatory action of 17beta-estradiol in the gilthead sea bream Sparus auratus.
J Exp Zool A Comp Exp Biol. 2004 Oct 01; 301(10):828-36.JE

Abstract

The osmoregulatory action of 17beta-estradiol (E2) was examined in the euryhaline teleost Sparus auratas. In a first set of experiments, fish were injected once with vegetable oil containing E2 (1, 2 and 5 microg/g body weight), transferred 12h after injection from sea water (SW, 38 ppt salinity) to hypersaline water (HSW, 55 ppt) or to brackish water (BW, 5 ppt salinity) and sampled 12h later (i.e. 24 h post-injection). In a second experiment, fish were injected intraperitoneally with coconut oil alone or containing E2 (10 microg/g body weight) and sampled after 5 days. In the same experiment, after 5 days of treatment, fish of each group were transferred to HSW, BW and SW and sampled 4 days later (9 days post-implant). Gill Na+,K+ -ATPase activity, plasma E2 levels, plasma osmolality, and plasma levels of ions (sodium and calcium), glucose, lactate, protein, triglyceride, and hepatosomatic index were examined. Transfer from SW to HSW produced no significant effects on any parameters assessed. E2 treatment did not affect any parameter. Transfer from SW to BW resulted in a significant decrease in plasma osmolality and plasma sodium but did not affect gill Na+,K+ -ATPase activity. A single dose of E2 attenuated the decrease in these parameters after transfer from SW to BW, but was without effect on gill Na+,K+ -ATPase activity. An implant of E2 (10 microg/g body weight) for 5 days significantly increased plasma calcium, hepatosomatic index, plasma metabolic parameters, and gill Na+,K+ -ATPase activity. In coconut oil-implanted (sham) fish, transfer from SW to HSW or BW during 4 days significantly elevated gill Na+,K+ -ATPase. Gill Na+,K+ -ATPase activity remained unaltered after transfer of E2-treated fish to HSW or BW. However, in E2-treated fish transferred from SW to SW (9 days in SW after E2-implant), gill Na+,K+ -ATPase activity decreased with respect to HSW- or BW-transferred fish. Shams transferred to HSW showed increased levels of lactate, protein, and trygliceride in plasma, while those transferred to BW only displayed increased trygliceride levels. E2-treated fish transferred to HSW showed higher protein levels without any change in other plasmatic parameters, while those transferred to BW displayed elevated plasma glucose levels but decreased osmolality and protein levels. These results substantiate a chronic stimulatory action of E2 on gill Na+,K+ -ATPase activity in the euryhaline teleost Sparus auratas.

Authors+Show Affiliations

Departamento de Biología, Facultad de Ciencias del Mar y Ambientales, Universidad de Cádiz, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15449347

Citation

Guzmán, José María, et al. "Osmoregulatory Action of 17beta-estradiol in the Gilthead Sea Bream Sparus Auratus." Journal of Experimental Zoology. Part A, Comparative Experimental Biology, vol. 301, no. 10, 2004, pp. 828-36.
Guzmán JM, Sangiao-Alvarellos S, Laiz-Carrión R, et al. Osmoregulatory action of 17beta-estradiol in the gilthead sea bream Sparus auratus. J Exp Zool A Comp Exp Biol. 2004;301(10):828-36.
Guzmán, J. M., Sangiao-Alvarellos, S., Laiz-Carrión, R., Míguez, J. M., Martín del Río, M. d. e. l. . P., Soengas, J. L., & Mancera, J. M. (2004). Osmoregulatory action of 17beta-estradiol in the gilthead sea bream Sparus auratus. Journal of Experimental Zoology. Part A, Comparative Experimental Biology, 301(10), 828-36.
Guzmán JM, et al. Osmoregulatory Action of 17beta-estradiol in the Gilthead Sea Bream Sparus Auratus. J Exp Zool A Comp Exp Biol. 2004 Oct 1;301(10):828-36. PubMed PMID: 15449347.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Osmoregulatory action of 17beta-estradiol in the gilthead sea bream Sparus auratus. AU - Guzmán,José María, AU - Sangiao-Alvarellos,Susana, AU - Laiz-Carrión,Raúl, AU - Míguez,Jesús M, AU - Martín del Río,María del Pilar, AU - Soengas,José Luis, AU - Mancera,Juan Miguel, PY - 2004/9/28/pubmed PY - 2004/12/22/medline PY - 2004/9/28/entrez SP - 828 EP - 36 JF - Journal of experimental zoology. Part A, Comparative experimental biology JO - J Exp Zool A Comp Exp Biol VL - 301 IS - 10 N2 - The osmoregulatory action of 17beta-estradiol (E2) was examined in the euryhaline teleost Sparus auratas. In a first set of experiments, fish were injected once with vegetable oil containing E2 (1, 2 and 5 microg/g body weight), transferred 12h after injection from sea water (SW, 38 ppt salinity) to hypersaline water (HSW, 55 ppt) or to brackish water (BW, 5 ppt salinity) and sampled 12h later (i.e. 24 h post-injection). In a second experiment, fish were injected intraperitoneally with coconut oil alone or containing E2 (10 microg/g body weight) and sampled after 5 days. In the same experiment, after 5 days of treatment, fish of each group were transferred to HSW, BW and SW and sampled 4 days later (9 days post-implant). Gill Na+,K+ -ATPase activity, plasma E2 levels, plasma osmolality, and plasma levels of ions (sodium and calcium), glucose, lactate, protein, triglyceride, and hepatosomatic index were examined. Transfer from SW to HSW produced no significant effects on any parameters assessed. E2 treatment did not affect any parameter. Transfer from SW to BW resulted in a significant decrease in plasma osmolality and plasma sodium but did not affect gill Na+,K+ -ATPase activity. A single dose of E2 attenuated the decrease in these parameters after transfer from SW to BW, but was without effect on gill Na+,K+ -ATPase activity. An implant of E2 (10 microg/g body weight) for 5 days significantly increased plasma calcium, hepatosomatic index, plasma metabolic parameters, and gill Na+,K+ -ATPase activity. In coconut oil-implanted (sham) fish, transfer from SW to HSW or BW during 4 days significantly elevated gill Na+,K+ -ATPase. Gill Na+,K+ -ATPase activity remained unaltered after transfer of E2-treated fish to HSW or BW. However, in E2-treated fish transferred from SW to SW (9 days in SW after E2-implant), gill Na+,K+ -ATPase activity decreased with respect to HSW- or BW-transferred fish. Shams transferred to HSW showed increased levels of lactate, protein, and trygliceride in plasma, while those transferred to BW only displayed increased trygliceride levels. E2-treated fish transferred to HSW showed higher protein levels without any change in other plasmatic parameters, while those transferred to BW displayed elevated plasma glucose levels but decreased osmolality and protein levels. These results substantiate a chronic stimulatory action of E2 on gill Na+,K+ -ATPase activity in the euryhaline teleost Sparus auratas. SN - 1548-8969 UR - https://www.unboundmedicine.com/medline/citation/15449347/Osmoregulatory_action_of_17beta_estradiol_in_the_gilthead_sea_bream_Sparus_auratus_ L2 - https://doi.org/10.1002/jez.a.102 DB - PRIME DP - Unbound Medicine ER -