Tags

Type your tag names separated by a space and hit enter

In vitro chemopreventive activity of Camellia sinensis, Ilex paraguariensis and Ardisia compressa tea extracts and selected polyphenols.
Mutat Res. 2004 Oct 04; 554(1-2):53-65.MR

Abstract

Several herbal teas contain bioactive compounds that have been associated with a lower risk of chronic diseases including cancer. The aim of this study was to evaluate the chemopreventive activity of tea aqueous extracts and selected constituent pure polyphenols using a battery of in vitro marker systems relevant for the prevention of cancer. The effects of (-) epigallocatechin gallate (EGCG), quercetin (Q), gallic acid (GA), green tea (GT, Camellia sinensis), ardisia tea (AT, Ardisia compressa) and mate tea (MT, Ilex paraguariensis) extracts were tested. Cytotoxicity, TPA-induced ornithine decarboxylase (ODC) and quinone reductase (QR) activities were evaluated in vitro using HepG2 cells. The topoisomerase inhibitory activity was also tested, using the Saccharomyces cerevisiae yeast system. Results suggest that MT, AT and GT are cytotoxic to the HepG2 cells, with MT demonstrating dominant cytotoxicity. EGCG showed greater cytotoxicity than Q and GA against HepG2 cells. The greatest inhibition (82%) of TPA-induced ODC activity was shown by Q, with 25 microM (IC50 = 11.90 microM). Topoisomerase II, but not topoisomerase I, was the cellular target of MT, AT, EGCG, Q and GA, which acted mainly as true catalytic inhibitors. The cytotoxic activity and the inhibition of topoisomerase II may contribute to the overall chemopreventive activity of AT and MT extracts. Ardisia and mate teas may thus share a public health potential as chemopreventive agents.

Authors+Show Affiliations

Department of Food Science and Human Nutrition, University of Illinois at Urbana-Champaign, 228 ERML, MC-051, 1201 W. Gregory Drive, 61801, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

15450404

Citation

Ramirez-Mares, Marco Vinicio, et al. "In Vitro Chemopreventive Activity of Camellia Sinensis, Ilex Paraguariensis and Ardisia Compressa Tea Extracts and Selected Polyphenols." Mutation Research, vol. 554, no. 1-2, 2004, pp. 53-65.
Ramirez-Mares MV, Chandra S, de Mejia EG. In vitro chemopreventive activity of Camellia sinensis, Ilex paraguariensis and Ardisia compressa tea extracts and selected polyphenols. Mutat Res. 2004;554(1-2):53-65.
Ramirez-Mares, M. V., Chandra, S., & de Mejia, E. G. (2004). In vitro chemopreventive activity of Camellia sinensis, Ilex paraguariensis and Ardisia compressa tea extracts and selected polyphenols. Mutation Research, 554(1-2), 53-65.
Ramirez-Mares MV, Chandra S, de Mejia EG. In Vitro Chemopreventive Activity of Camellia Sinensis, Ilex Paraguariensis and Ardisia Compressa Tea Extracts and Selected Polyphenols. Mutat Res. 2004 Oct 4;554(1-2):53-65. PubMed PMID: 15450404.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vitro chemopreventive activity of Camellia sinensis, Ilex paraguariensis and Ardisia compressa tea extracts and selected polyphenols. AU - Ramirez-Mares,Marco Vinicio, AU - Chandra,Sonia, AU - de Mejia,Elvira Gonzalez, PY - 2003/10/28/received PY - 2004/01/26/revised PY - 2004/03/02/accepted PY - 2004/9/29/pubmed PY - 2004/11/4/medline PY - 2004/9/29/entrez SP - 53 EP - 65 JF - Mutation research JO - Mutat Res VL - 554 IS - 1-2 N2 - Several herbal teas contain bioactive compounds that have been associated with a lower risk of chronic diseases including cancer. The aim of this study was to evaluate the chemopreventive activity of tea aqueous extracts and selected constituent pure polyphenols using a battery of in vitro marker systems relevant for the prevention of cancer. The effects of (-) epigallocatechin gallate (EGCG), quercetin (Q), gallic acid (GA), green tea (GT, Camellia sinensis), ardisia tea (AT, Ardisia compressa) and mate tea (MT, Ilex paraguariensis) extracts were tested. Cytotoxicity, TPA-induced ornithine decarboxylase (ODC) and quinone reductase (QR) activities were evaluated in vitro using HepG2 cells. The topoisomerase inhibitory activity was also tested, using the Saccharomyces cerevisiae yeast system. Results suggest that MT, AT and GT are cytotoxic to the HepG2 cells, with MT demonstrating dominant cytotoxicity. EGCG showed greater cytotoxicity than Q and GA against HepG2 cells. The greatest inhibition (82%) of TPA-induced ODC activity was shown by Q, with 25 microM (IC50 = 11.90 microM). Topoisomerase II, but not topoisomerase I, was the cellular target of MT, AT, EGCG, Q and GA, which acted mainly as true catalytic inhibitors. The cytotoxic activity and the inhibition of topoisomerase II may contribute to the overall chemopreventive activity of AT and MT extracts. Ardisia and mate teas may thus share a public health potential as chemopreventive agents. SN - 0027-5107 UR - https://www.unboundmedicine.com/medline/citation/15450404/In_vitro_chemopreventive_activity_of_Camellia_sinensis_Ilex_paraguariensis_and_Ardisia_compressa_tea_extracts_and_selected_polyphenols_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S002751070400079X DB - PRIME DP - Unbound Medicine ER -