Tags

Type your tag names separated by a space and hit enter

Diagnosis of chronic alcohol consumption. Hair analysis for ethyl-glucuronide.
Forensic Sci Int. 2004 Oct 29; 145(2-3):161-6.FS

Abstract

This paper describes a procedure for the detection and quantification of ethyl-glucuronide (EtG) in hair samples. During method development the efficacy of extraction of EtG from hair was compared in four extraction methods: (a) methanol; (b) methanol:water (1:1); (c) water; and (d) water:trifluoroacetic acid (9:1). In addition, three derivatizing agents were compared as well: N,O-bistrimethylsilyl-trifluoroacetamide (BSTFA): trimethylchlorosilane (TMCS) (99:1), pentafluoropropionic anhydride (PFPA) and heptafluorobutyric anhydride (HFBA). Water was found to be the best extracting solvent and PFPA the best derivatizing agent. Both provided the highest recoveries, with cleaner extracts and more stable derivatives. The final method is as follows: about 100mg of hair are sequentially washed with water and acetone. The decontaminated sample is finely cut with scissors, then the deuterated internal standard (EtG-d5) and 2 mL of water are added. After sonication for 2 h, the sample is maintained at room temperature overnight. Derivatization is performed with PFPA. Derivatives are injected into a GC-MS system in the electronic impact mode. The method shows linearity over the range of concentrations from 0.050 to 5 ng/mg. Detection and quantification limits are 0.025 and 0.050 ng/mg, respectively. Mean recoveries for the three studied concentrations (low, medium and high) are higher than 87%. The coefficients of variation in intra- and inter-assay precision are always lower than 7%. The method is being routinely applied in our lab for the diagnosis of chronic alcohol consumption.

Authors+Show Affiliations

Instituto Nacional de Toxicología, P.O. Box 863, 41080 Sevilla, Spain. cjurado@arrakis.esNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15451088

Citation

Jurado, C, et al. "Diagnosis of Chronic Alcohol Consumption. Hair Analysis for Ethyl-glucuronide." Forensic Science International, vol. 145, no. 2-3, 2004, pp. 161-6.
Jurado C, Soriano T, Giménez MP, et al. Diagnosis of chronic alcohol consumption. Hair analysis for ethyl-glucuronide. Forensic Sci Int. 2004;145(2-3):161-6.
Jurado, C., Soriano, T., Giménez, M. P., & Menéndez, M. (2004). Diagnosis of chronic alcohol consumption. Hair analysis for ethyl-glucuronide. Forensic Science International, 145(2-3), 161-6.
Jurado C, et al. Diagnosis of Chronic Alcohol Consumption. Hair Analysis for Ethyl-glucuronide. Forensic Sci Int. 2004 Oct 29;145(2-3):161-6. PubMed PMID: 15451088.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Diagnosis of chronic alcohol consumption. Hair analysis for ethyl-glucuronide. AU - Jurado,C, AU - Soriano,T, AU - Giménez,M P, AU - Menéndez,M, PY - 2004/9/29/pubmed PY - 2004/12/22/medline PY - 2004/9/29/entrez SP - 161 EP - 6 JF - Forensic science international JO - Forensic Sci Int VL - 145 IS - 2-3 N2 - This paper describes a procedure for the detection and quantification of ethyl-glucuronide (EtG) in hair samples. During method development the efficacy of extraction of EtG from hair was compared in four extraction methods: (a) methanol; (b) methanol:water (1:1); (c) water; and (d) water:trifluoroacetic acid (9:1). In addition, three derivatizing agents were compared as well: N,O-bistrimethylsilyl-trifluoroacetamide (BSTFA): trimethylchlorosilane (TMCS) (99:1), pentafluoropropionic anhydride (PFPA) and heptafluorobutyric anhydride (HFBA). Water was found to be the best extracting solvent and PFPA the best derivatizing agent. Both provided the highest recoveries, with cleaner extracts and more stable derivatives. The final method is as follows: about 100mg of hair are sequentially washed with water and acetone. The decontaminated sample is finely cut with scissors, then the deuterated internal standard (EtG-d5) and 2 mL of water are added. After sonication for 2 h, the sample is maintained at room temperature overnight. Derivatization is performed with PFPA. Derivatives are injected into a GC-MS system in the electronic impact mode. The method shows linearity over the range of concentrations from 0.050 to 5 ng/mg. Detection and quantification limits are 0.025 and 0.050 ng/mg, respectively. Mean recoveries for the three studied concentrations (low, medium and high) are higher than 87%. The coefficients of variation in intra- and inter-assay precision are always lower than 7%. The method is being routinely applied in our lab for the diagnosis of chronic alcohol consumption. SN - 0379-0738 UR - https://www.unboundmedicine.com/medline/citation/15451088/Diagnosis_of_chronic_alcohol_consumption__Hair_analysis_for_ethyl_glucuronide_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0379073804002464 DB - PRIME DP - Unbound Medicine ER -