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Transgenic expression of Cre recombinase from the tyrosine hydroxylase locus.
Genesis. 2004 Oct; 40(2):67-73.G

Abstract

Catecholaminergic neurons are affected in several neurological and psychiatric diseases. Tyrosine hydroxylase (TH) is the first, rate-limiting enzyme in catecholamine synthesis. We report a knockin mouse expressing Cre-recombinase from the 3'-untranslated region of the endogenous Th gene by means of an internal ribosomal entry sequence (IRES). The resulting Cre expression matches the normal pattern of TH expression, while the pattern and level of TH are not altered in the knockin mouse. Crossings with two different LacZ reporter mice demonstrated Cre-mediated genomic recombination in TH expressing tissues. In addition, LacZ was found in some unexpected cell populations (including oocytes), indicating recombination due to transient developmental TH expression. Our novel knockin mouse can be used for generation of tissue-specific or general knockouts (depending on scheme of crossing) in mice carrying genes flanked by loxP sites. This knockin mouse can also be used for tracing cell lineages expressing TH during development.

Authors+Show Affiliations

Department of Neuroscience, Developmental Neuroscience Unit, Uppsala University, Biomedical Center, SE-751 23 Uppsala, Sweden.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15452869

Citation

Lindeberg, Jonas, et al. "Transgenic Expression of Cre Recombinase From the Tyrosine Hydroxylase Locus." Genesis (New York, N.Y. : 2000), vol. 40, no. 2, 2004, pp. 67-73.
Lindeberg J, Usoskin D, Bengtsson H, et al. Transgenic expression of Cre recombinase from the tyrosine hydroxylase locus. Genesis. 2004;40(2):67-73.
Lindeberg, J., Usoskin, D., Bengtsson, H., Gustafsson, A., Kylberg, A., Söderström, S., & Ebendal, T. (2004). Transgenic expression of Cre recombinase from the tyrosine hydroxylase locus. Genesis (New York, N.Y. : 2000), 40(2), 67-73.
Lindeberg J, et al. Transgenic Expression of Cre Recombinase From the Tyrosine Hydroxylase Locus. Genesis. 2004;40(2):67-73. PubMed PMID: 15452869.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Transgenic expression of Cre recombinase from the tyrosine hydroxylase locus. AU - Lindeberg,Jonas, AU - Usoskin,Dmitry, AU - Bengtsson,Henrik, AU - Gustafsson,Anna, AU - Kylberg,Annika, AU - Söderström,Stine, AU - Ebendal,Ted, PY - 2004/9/29/pubmed PY - 2005/3/12/medline PY - 2004/9/29/entrez SP - 67 EP - 73 JF - Genesis (New York, N.Y. : 2000) JO - Genesis VL - 40 IS - 2 N2 - Catecholaminergic neurons are affected in several neurological and psychiatric diseases. Tyrosine hydroxylase (TH) is the first, rate-limiting enzyme in catecholamine synthesis. We report a knockin mouse expressing Cre-recombinase from the 3'-untranslated region of the endogenous Th gene by means of an internal ribosomal entry sequence (IRES). The resulting Cre expression matches the normal pattern of TH expression, while the pattern and level of TH are not altered in the knockin mouse. Crossings with two different LacZ reporter mice demonstrated Cre-mediated genomic recombination in TH expressing tissues. In addition, LacZ was found in some unexpected cell populations (including oocytes), indicating recombination due to transient developmental TH expression. Our novel knockin mouse can be used for generation of tissue-specific or general knockouts (depending on scheme of crossing) in mice carrying genes flanked by loxP sites. This knockin mouse can also be used for tracing cell lineages expressing TH during development. SN - 1526-954X UR - https://www.unboundmedicine.com/medline/citation/15452869/Transgenic_expression_of_Cre_recombinase_from_the_tyrosine_hydroxylase_locus_ L2 - https://doi.org/10.1002/gene.20065 DB - PRIME DP - Unbound Medicine ER -