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Generation of novel conditional and hypomorphic alleles of the Smad2 gene.
Genesis. 2004 Oct; 40(2):118-123.G

Abstract

Smad2 is an intracellular mediator of the transforming growth factor beta signaling (TGFbeta) pathway. It has been previously shown that, in the mouse, ablation of functional Smad2 results in embryonic lethality due to gastrulation defects. To circumvent the early lethality and study the spatially and temporally specific functions of Smad2, we utilized the Cre-loxP system to generate a Smad2 conditional allele. Here we show that a conditional allele, Smad2(flox), was generated. In this allele, exons 9 and 10 are flanked by loxP sites and the gene is functionally wildtype. Cre-mediated recombination results in a deletion allele which phenocopies our previously reported Smad2(DeltaC) null mutation. To generate this conditional allele, we first made a targeted mutation which introduced a floxed neo cassette into intron 10. This allele (Smad2(3loxP)) functions hypomorphically when placed opposite a null allele, and unlike the other published Smad2 hypomorphic allele, can be maintained in the homozygous state.

Authors+Show Affiliations

Molecular, Cellular, and Developmental Biology Program, The Ohio State University, Columbus, OH 43210, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15452874

Citation

Liu, Ye, et al. "Generation of Novel Conditional and Hypomorphic Alleles of the Smad2 Gene." Genesis (New York, N.Y. : 2000), vol. 40, no. 2, 2004, pp. 118-123.
Liu Y, Festing MH, Hester M, et al. Generation of novel conditional and hypomorphic alleles of the Smad2 gene. Genesis. 2004;40(2):118-123.
Liu, Y., Festing, M. H., Hester, M., Thompson, J. C., & Weinstein, M. (2004). Generation of novel conditional and hypomorphic alleles of the Smad2 gene. Genesis (New York, N.Y. : 2000), 40(2), 118-123.
Liu Y, et al. Generation of Novel Conditional and Hypomorphic Alleles of the Smad2 Gene. Genesis. 2004;40(2):118-123. PubMed PMID: 15452874.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Generation of novel conditional and hypomorphic alleles of the Smad2 gene. AU - Liu,Ye, AU - Festing,Maria H, AU - Hester,Mark, AU - Thompson,John C, AU - Weinstein,Michael, PY - 2004/9/29/pubmed PY - 2005/3/12/medline PY - 2004/9/29/entrez SP - 118 EP - 123 JF - Genesis (New York, N.Y. : 2000) JO - Genesis VL - 40 IS - 2 N2 - Smad2 is an intracellular mediator of the transforming growth factor beta signaling (TGFbeta) pathway. It has been previously shown that, in the mouse, ablation of functional Smad2 results in embryonic lethality due to gastrulation defects. To circumvent the early lethality and study the spatially and temporally specific functions of Smad2, we utilized the Cre-loxP system to generate a Smad2 conditional allele. Here we show that a conditional allele, Smad2(flox), was generated. In this allele, exons 9 and 10 are flanked by loxP sites and the gene is functionally wildtype. Cre-mediated recombination results in a deletion allele which phenocopies our previously reported Smad2(DeltaC) null mutation. To generate this conditional allele, we first made a targeted mutation which introduced a floxed neo cassette into intron 10. This allele (Smad2(3loxP)) functions hypomorphically when placed opposite a null allele, and unlike the other published Smad2 hypomorphic allele, can be maintained in the homozygous state. SN - 1526-954X UR - https://www.unboundmedicine.com/medline/citation/15452874/Generation_of_novel_conditional_and_hypomorphic_alleles_of_the_Smad2_gene_ L2 - https://doi.org/10.1002/gene.20072 DB - PRIME DP - Unbound Medicine ER -