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Morphological changes and oxidative stress in rat prostate exposed to a non-carcinogenic dose of cadmium.
Toxicol Lett. 2004 Nov 28; 153(3):365-76.TL

Abstract

Cadmium chloride is an environmental toxicant implicated in human prostate carcinogenesis. The mechanism of its toxicity is far from fully understood. This study evaluates the effect of exposure to an oral non-carcinogenic dose of cadmium (15 ppm in drinking water for three months) on different parameters of the ventral prostatic lobe of normal and exposed rats. We analyzed the histology by optic light microscopy, activities of antioxidant enzymes (CAT, SOD, GPx and G-6-PDH), expression of iNOS and COX-2 by Western blot, expression of MT-I, MT-II, IGF-I, IGF-BP5 and rtert by RT-PCR. Histological changes were found: the height of the cells decreased, acinar lumen were enlarged and they lost the typical invaginations. Lipoperoxidation was increased in the Cd group and the antioxidant enzymes changed their activities: SOD increased, CAT and G-6-PDH decreased and GPx did not show variations. iNOS and COX-2 did not change their expressions. MT-I and IGF-BP5 mRNA increased while MT-II, IGF-I and rtert did not show variations. Cd exposure induces important morphological changes in the prostate, which could be a consequence of lipoperoxidation and oxidative stress, which are not related to iNOS and COX-2. The histology suggests an involution state of the gland, confirmed by the expression of IGF-I, IGF-BP5 and rtert.

Authors+Show Affiliations

Laboratory of Molecular Biochemistry, Faculty of Chemistry, Biochemistry and Pharmacy, National University of San Luis, Avenida Ejercito de los Andes 950, 5700 San Luis, Argentina. salvarez@unsl.edu.arNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15454312

Citation

Alvarez, Silvina M., et al. "Morphological Changes and Oxidative Stress in Rat Prostate Exposed to a Non-carcinogenic Dose of Cadmium." Toxicology Letters, vol. 153, no. 3, 2004, pp. 365-76.
Alvarez SM, Gómez NN, Scardapane L, et al. Morphological changes and oxidative stress in rat prostate exposed to a non-carcinogenic dose of cadmium. Toxicol Lett. 2004;153(3):365-76.
Alvarez, S. M., Gómez, N. N., Scardapane, L., Zirulnik, F., Martínez, D., & Giménez, M. S. (2004). Morphological changes and oxidative stress in rat prostate exposed to a non-carcinogenic dose of cadmium. Toxicology Letters, 153(3), 365-76.
Alvarez SM, et al. Morphological Changes and Oxidative Stress in Rat Prostate Exposed to a Non-carcinogenic Dose of Cadmium. Toxicol Lett. 2004 Nov 28;153(3):365-76. PubMed PMID: 15454312.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Morphological changes and oxidative stress in rat prostate exposed to a non-carcinogenic dose of cadmium. AU - Alvarez,Silvina M, AU - Gómez,Nidia N, AU - Scardapane,Luis, AU - Zirulnik,Fanny, AU - Martínez,Dante, AU - Giménez,María Sofía, PY - 2004/03/25/received PY - 2004/06/16/revised PY - 2004/06/17/accepted PY - 2004/9/30/pubmed PY - 2004/12/16/medline PY - 2004/9/30/entrez SP - 365 EP - 76 JF - Toxicology letters JO - Toxicol Lett VL - 153 IS - 3 N2 - Cadmium chloride is an environmental toxicant implicated in human prostate carcinogenesis. The mechanism of its toxicity is far from fully understood. This study evaluates the effect of exposure to an oral non-carcinogenic dose of cadmium (15 ppm in drinking water for three months) on different parameters of the ventral prostatic lobe of normal and exposed rats. We analyzed the histology by optic light microscopy, activities of antioxidant enzymes (CAT, SOD, GPx and G-6-PDH), expression of iNOS and COX-2 by Western blot, expression of MT-I, MT-II, IGF-I, IGF-BP5 and rtert by RT-PCR. Histological changes were found: the height of the cells decreased, acinar lumen were enlarged and they lost the typical invaginations. Lipoperoxidation was increased in the Cd group and the antioxidant enzymes changed their activities: SOD increased, CAT and G-6-PDH decreased and GPx did not show variations. iNOS and COX-2 did not change their expressions. MT-I and IGF-BP5 mRNA increased while MT-II, IGF-I and rtert did not show variations. Cd exposure induces important morphological changes in the prostate, which could be a consequence of lipoperoxidation and oxidative stress, which are not related to iNOS and COX-2. The histology suggests an involution state of the gland, confirmed by the expression of IGF-I, IGF-BP5 and rtert. SN - 0378-4274 UR - https://www.unboundmedicine.com/medline/citation/15454312/Morphological_changes_and_oxidative_stress_in_rat_prostate_exposed_to_a_non_carcinogenic_dose_of_cadmium_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-4274(04)00315-7 DB - PRIME DP - Unbound Medicine ER -