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Radiation hybrid mapping of the species cytoplasm-specific (scsae) gene in wheat.
Genetics. 2004 Sep; 168(1):415-23.G

Abstract

Radiation hybrid (RH) mapping is based on radiation-induced chromosome breakage and analysis of chromosome segment retention or loss using molecular markers. In durum wheat (Triticum turgidum L., AABB), an alloplasmic durum line [(lo) durum] has been identified with chromosome 1D of T. aestivum L. (AABBDD) carrying the species cytoplasm-specific (scsae) gene. The chromosome 1D of this line segregates as a whole without recombination, precluding the use of conventional genome mapping. A radiation hybrid mapping population was developed from a hemizygous (lo) scsae--line using 35 krad gamma rays. The analysis of 87 individuals of this population with 39 molecular markers mapped on chromosome 1D revealed 88 radiation-induced breaks in this chromosome. This number of chromosome 1D breaks is eight times higher than the number of previously identified breaks and should result in a 10-fold increase in mapping resolution compared to what was previously possible. The analysis of molecular marker retention in our radiation hybrid mapping panel allowed the localization of scsae and 8 linked markers on the long arm of chromosome 1D. This constitutes the first report of using RH mapping to localize a gene in wheat and illustrates that this approach is feasible in a species with a large complex genome.

Authors+Show Affiliations

Department of Plant Sciences, North Dakota State University, Fargo 58105, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

15454553

Citation

Hossain, Khwaja G., et al. "Radiation Hybrid Mapping of the Species Cytoplasm-specific (scsae) Gene in Wheat." Genetics, vol. 168, no. 1, 2004, pp. 415-23.
Hossain KG, Riera-Lizarazu O, Kalavacharla V, et al. Radiation hybrid mapping of the species cytoplasm-specific (scsae) gene in wheat. Genetics. 2004;168(1):415-23.
Hossain, K. G., Riera-Lizarazu, O., Kalavacharla, V., Vales, M. I., Maan, S. S., & Kianian, S. F. (2004). Radiation hybrid mapping of the species cytoplasm-specific (scsae) gene in wheat. Genetics, 168(1), 415-23.
Hossain KG, et al. Radiation Hybrid Mapping of the Species Cytoplasm-specific (scsae) Gene in Wheat. Genetics. 2004;168(1):415-23. PubMed PMID: 15454553.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Radiation hybrid mapping of the species cytoplasm-specific (scsae) gene in wheat. AU - Hossain,Khwaja G, AU - Riera-Lizarazu,Oscar, AU - Kalavacharla,Venugopal, AU - Vales,M Isabel, AU - Maan,Schivcharan S, AU - Kianian,Shahryar F, PY - 2004/9/30/pubmed PY - 2005/4/13/medline PY - 2004/9/30/entrez SP - 415 EP - 23 JF - Genetics JO - Genetics VL - 168 IS - 1 N2 - Radiation hybrid (RH) mapping is based on radiation-induced chromosome breakage and analysis of chromosome segment retention or loss using molecular markers. In durum wheat (Triticum turgidum L., AABB), an alloplasmic durum line [(lo) durum] has been identified with chromosome 1D of T. aestivum L. (AABBDD) carrying the species cytoplasm-specific (scsae) gene. The chromosome 1D of this line segregates as a whole without recombination, precluding the use of conventional genome mapping. A radiation hybrid mapping population was developed from a hemizygous (lo) scsae--line using 35 krad gamma rays. The analysis of 87 individuals of this population with 39 molecular markers mapped on chromosome 1D revealed 88 radiation-induced breaks in this chromosome. This number of chromosome 1D breaks is eight times higher than the number of previously identified breaks and should result in a 10-fold increase in mapping resolution compared to what was previously possible. The analysis of molecular marker retention in our radiation hybrid mapping panel allowed the localization of scsae and 8 linked markers on the long arm of chromosome 1D. This constitutes the first report of using RH mapping to localize a gene in wheat and illustrates that this approach is feasible in a species with a large complex genome. SN - 0016-6731 UR - https://www.unboundmedicine.com/medline/citation/15454553/Radiation_hybrid_mapping_of_the_species_cytoplasm_specific__scsae__gene_in_wheat_ L2 - https://academic.oup.com/genetics/article-lookup/doi/10.1534/genetics.103.022590 DB - PRIME DP - Unbound Medicine ER -