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Using radioligand-binding assays to measure tissue transglutaminase autoantibodies in young children.
Acta Paediatr 2004; 93(8):1046-51AP

Abstract

AIM

To measure autoantibodies against tissue transglutaminase (tTG) in young children prospectively screened for coeliac disease (CD).

METHODS

In total, 652 children aged 2.9 (2.5-4.2) y were analysed for IgA-tTG and IgG-tTG with radioligand-binding assays and IgA endomysial antibodies (EMA) by indirect immunofluorescence. Antibody-positive children were retested after 1.2 (range 0.2-1.9) y. Intestinal biopsy was performed on children with persistently high antibody levels.

RESULTS

In total, 3.2% (95% CI: 1.9-4.6%) of the 652 children were positive for at least one antibody at baseline: 2.5% (95% CI: 1.3-3.7%) for IgA-tTG, 1.7% (95% CI: 0.7-2.7%) for IgG-tTG and 2.9% (95% CI: 1.6-4.2%) for IgA-EMA, respectively. Ten children were positive for all three antibodies, five for both IgA-tTG and EMA, four for EMA only, one for IgA-tTG and another for IgG-tTG. IgA-EMA titres correlated with IgA-tTG levels (r = 0.73, p = 0.0003). At follow-up, seven of 20 children remained positive for all three antibodies, three for IgA-tTG only, one for both IgA-tTG and EMA, one for IgA-tTG and IgG-tTG, and the remaining child refused further participation. Three biopsies showed villous atrophy, two increased intraepithelial lymphocytes and two normal findings. Biopsy was not performed in four children with low or declining tTG antibody levels at follow-up and in one child who declined. CD was evident in 0.5% (95% CI: 0.0-1.0%) (3/652).

CONCLUSION

This study revealed a high number of young children positive for tTG antibodies as well as EMA, but the majority showed declining levels in both antibodies over time. We suggest using radioligand-binding assays for quantitative measurement of tTG antibodies when change in antibody levels is studied in young children.

Authors+Show Affiliations

Department of Paediatrics, Malmö University Hospital, Lund University, Malmö Sweden. daniel.agardh@home.seNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Studies
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15456194

Citation

Agardh, D, et al. "Using Radioligand-binding Assays to Measure Tissue Transglutaminase Autoantibodies in Young Children." Acta Paediatrica (Oslo, Norway : 1992), vol. 93, no. 8, 2004, pp. 1046-51.
Agardh D, Carlsson A, Lynch K, et al. Using radioligand-binding assays to measure tissue transglutaminase autoantibodies in young children. Acta Paediatr. 2004;93(8):1046-51.
Agardh, D., Carlsson, A., Lynch, K., Axelsson, I., Lemmark, A., & Ivarsson, S. A. (2004). Using radioligand-binding assays to measure tissue transglutaminase autoantibodies in young children. Acta Paediatrica (Oslo, Norway : 1992), 93(8), pp. 1046-51.
Agardh D, et al. Using Radioligand-binding Assays to Measure Tissue Transglutaminase Autoantibodies in Young Children. Acta Paediatr. 2004;93(8):1046-51. PubMed PMID: 15456194.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Using radioligand-binding assays to measure tissue transglutaminase autoantibodies in young children. AU - Agardh,D, AU - Carlsson,A, AU - Lynch,K, AU - Axelsson,I, AU - Lemmark,A, AU - Ivarsson,S A, PY - 2004/10/1/pubmed PY - 2004/12/24/medline PY - 2004/10/1/entrez SP - 1046 EP - 51 JF - Acta paediatrica (Oslo, Norway : 1992) JO - Acta Paediatr. VL - 93 IS - 8 N2 - AIM: To measure autoantibodies against tissue transglutaminase (tTG) in young children prospectively screened for coeliac disease (CD). METHODS: In total, 652 children aged 2.9 (2.5-4.2) y were analysed for IgA-tTG and IgG-tTG with radioligand-binding assays and IgA endomysial antibodies (EMA) by indirect immunofluorescence. Antibody-positive children were retested after 1.2 (range 0.2-1.9) y. Intestinal biopsy was performed on children with persistently high antibody levels. RESULTS: In total, 3.2% (95% CI: 1.9-4.6%) of the 652 children were positive for at least one antibody at baseline: 2.5% (95% CI: 1.3-3.7%) for IgA-tTG, 1.7% (95% CI: 0.7-2.7%) for IgG-tTG and 2.9% (95% CI: 1.6-4.2%) for IgA-EMA, respectively. Ten children were positive for all three antibodies, five for both IgA-tTG and EMA, four for EMA only, one for IgA-tTG and another for IgG-tTG. IgA-EMA titres correlated with IgA-tTG levels (r = 0.73, p = 0.0003). At follow-up, seven of 20 children remained positive for all three antibodies, three for IgA-tTG only, one for both IgA-tTG and EMA, one for IgA-tTG and IgG-tTG, and the remaining child refused further participation. Three biopsies showed villous atrophy, two increased intraepithelial lymphocytes and two normal findings. Biopsy was not performed in four children with low or declining tTG antibody levels at follow-up and in one child who declined. CD was evident in 0.5% (95% CI: 0.0-1.0%) (3/652). CONCLUSION: This study revealed a high number of young children positive for tTG antibodies as well as EMA, but the majority showed declining levels in both antibodies over time. We suggest using radioligand-binding assays for quantitative measurement of tTG antibodies when change in antibody levels is studied in young children. SN - 0803-5253 UR - https://www.unboundmedicine.com/medline/citation/15456194/Using_radioligand_binding_assays_to_measure_tissue_transglutaminase_autoantibodies_in_young_children_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0803-5253&date=2004&volume=93&issue=8&spage=1046 DB - PRIME DP - Unbound Medicine ER -