Tags

Type your tag names separated by a space and hit enter

Montelukast modulates lung CysLT(1) receptor expression and eosinophilic inflammation in asthmatic mice.
Acta Pharmacol Sin. 2004 Oct; 25(10):1341-6.AP

Abstract

AIM

To determine the expressions of cysteinyl leukotriene receptors, CysLT1 and CysLT2, in airway eosinophilic inflammation of OVA-induced asthmatic mice and the modulation by montelukast, a CysLT1 receptor antagonist.

METHODS

Asthma model was induced by chronic exposure to ovalbumin (OVA) in C57BL/6 mice. The eosinophils in bronchoalveolar lavage (BAL) fluid and lung tissues were counted, IL-5 level in BAL fluid was measured, and CysLT1 and CysLT2 receptor mRNA expressions were detected by semi-quantitative RT-PCR.

RESULTS

Montelukast (6 mg/kg, once per day for 20 d) significantly suppressed the increased eosinophils in BAL fluid and lung tissue, and increased IL-5 level in BAL fluid in OVA challenged mice. OVA challenge increased CysLT1 but decreased CysLT2 receptor mRNA expression. Montelukast inhibited the increased CysLT1 but not the reduced CysLT2 expression after OVA challenge.

CONCLUSION

CysLT receptors are modulated immunologically, and montelukast inhibits up-regulation of CysLT1 receptor and airway eosinophilic inflammation in asthmatic mice.

Authors+Show Affiliations

Department of Pharmacology, Zhejiang University School of Medicine, Hangzhou 310031, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15456537

Citation

Zhang, Yan-Jun, et al. "Montelukast Modulates Lung CysLT(1) Receptor Expression and Eosinophilic Inflammation in Asthmatic Mice." Acta Pharmacologica Sinica, vol. 25, no. 10, 2004, pp. 1341-6.
Zhang YJ, Zhang L, Wang SB, et al. Montelukast modulates lung CysLT(1) receptor expression and eosinophilic inflammation in asthmatic mice. Acta Pharmacol Sin. 2004;25(10):1341-6.
Zhang, Y. J., Zhang, L., Wang, S. B., Shen, H. H., & Wei, E. Q. (2004). Montelukast modulates lung CysLT(1) receptor expression and eosinophilic inflammation in asthmatic mice. Acta Pharmacologica Sinica, 25(10), 1341-6.
Zhang YJ, et al. Montelukast Modulates Lung CysLT(1) Receptor Expression and Eosinophilic Inflammation in Asthmatic Mice. Acta Pharmacol Sin. 2004;25(10):1341-6. PubMed PMID: 15456537.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Montelukast modulates lung CysLT(1) receptor expression and eosinophilic inflammation in asthmatic mice. AU - Zhang,Yan-Jun, AU - Zhang,Lei, AU - Wang,Shao-Bin, AU - Shen,Hua-Hao, AU - Wei,Er-Qing, PY - 2004/10/1/pubmed PY - 2005/1/29/medline PY - 2004/10/1/entrez SP - 1341 EP - 6 JF - Acta pharmacologica Sinica JO - Acta Pharmacol Sin VL - 25 IS - 10 N2 - AIM: To determine the expressions of cysteinyl leukotriene receptors, CysLT1 and CysLT2, in airway eosinophilic inflammation of OVA-induced asthmatic mice and the modulation by montelukast, a CysLT1 receptor antagonist. METHODS: Asthma model was induced by chronic exposure to ovalbumin (OVA) in C57BL/6 mice. The eosinophils in bronchoalveolar lavage (BAL) fluid and lung tissues were counted, IL-5 level in BAL fluid was measured, and CysLT1 and CysLT2 receptor mRNA expressions were detected by semi-quantitative RT-PCR. RESULTS: Montelukast (6 mg/kg, once per day for 20 d) significantly suppressed the increased eosinophils in BAL fluid and lung tissue, and increased IL-5 level in BAL fluid in OVA challenged mice. OVA challenge increased CysLT1 but decreased CysLT2 receptor mRNA expression. Montelukast inhibited the increased CysLT1 but not the reduced CysLT2 expression after OVA challenge. CONCLUSION: CysLT receptors are modulated immunologically, and montelukast inhibits up-regulation of CysLT1 receptor and airway eosinophilic inflammation in asthmatic mice. SN - 1671-4083 UR - https://www.unboundmedicine.com/medline/citation/15456537/Montelukast_modulates_lung_CysLT_1__receptor_expression_and_eosinophilic_inflammation_in_asthmatic_mice_ L2 - http://www.chinaphar.com/1671-4083/25/1341.pdf DB - PRIME DP - Unbound Medicine ER -