Tags

Type your tag names separated by a space and hit enter

Compartmentalization established by claudin-11-based tight junctions in stria vascularis is required for hearing through generation of endocochlear potential.
J Cell Sci. 2004 Oct 01; 117(Pt 21):5087-96.JC

Abstract

Claudins are cell adhesion molecules working at tight junctions (TJs) that are directly involved in compartmentalization in multicellular organisms. The cochlea includes a rather peculiar compartment filled with endolymph. This compartment is characterized by high K+ concentration (approximately 150 mM) and a positive endocochlear potential (approximately 90 mV; EP), both indispensable conditions for cochlear hair cells to transduce acoustic stimuli to electrical signals. These conditions are thought to be generated by the stria vascularis, which is adjacent to the endolymph compartment. The stria vascularis itself constitutes an isolated compartment delineated by two epithelial barriers, marginal and basal cell layers. Because TJs of basal cells are primarily composed of claudin-11, claudin-11-deficient (Cld11-/-) mice were generated with an expectation that the compartmentalization in stria vascularis in these mice would be affected. Auditory brainstem response measurements revealed that Cld11-/- mice suffered from deafness; although no obvious gross morphological malformations were detected in Cld11-/- cochlea, freeze-fracture replica electron microscopy showed that TJs disappeared from basal cells of the stria vascularis. In good agreement with this, tracer experiments showed that the basal cell barrier was destroyed without affecting the marginal cell barrier. Importantly, in the endolymph compartment of Cld11-/- cochlea, the K+ concentration was maintained around the normal level (approximately 150 mM), whereas the EP was suppressed down to approximately 30 mV. These findings indicated that the establishment of the stria vascularis compartment, especially the basal cell barrier, is indispensable for hearing ability through the generation/maintenance of EP but not of a high K+ concentration in the endolymph.

Authors+Show Affiliations

Department of Cell Biology, Faculty of Medicine, Kyoto University, Sakyo-ku, 606-8501, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15456848

Citation

Kitajiri, Shin-ichiro, et al. "Compartmentalization Established By Claudin-11-based Tight Junctions in Stria Vascularis Is Required for Hearing Through Generation of Endocochlear Potential." Journal of Cell Science, vol. 117, no. Pt 21, 2004, pp. 5087-96.
Kitajiri S, Miyamoto T, Mineharu A, et al. Compartmentalization established by claudin-11-based tight junctions in stria vascularis is required for hearing through generation of endocochlear potential. J Cell Sci. 2004;117(Pt 21):5087-96.
Kitajiri, S., Miyamoto, T., Mineharu, A., Sonoda, N., Furuse, K., Hata, M., Sasaki, H., Mori, Y., Kubota, T., Ito, J., Furuse, M., & Tsukita, S. (2004). Compartmentalization established by claudin-11-based tight junctions in stria vascularis is required for hearing through generation of endocochlear potential. Journal of Cell Science, 117(Pt 21), 5087-96.
Kitajiri S, et al. Compartmentalization Established By Claudin-11-based Tight Junctions in Stria Vascularis Is Required for Hearing Through Generation of Endocochlear Potential. J Cell Sci. 2004 Oct 1;117(Pt 21):5087-96. PubMed PMID: 15456848.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Compartmentalization established by claudin-11-based tight junctions in stria vascularis is required for hearing through generation of endocochlear potential. AU - Kitajiri,Shin-ichiro, AU - Miyamoto,Tatsuo, AU - Mineharu,Akihito, AU - Sonoda,Noriyuki, AU - Furuse,Kyoko, AU - Hata,Masaki, AU - Sasaki,Hiroyuki, AU - Mori,Yoshiaki, AU - Kubota,Takahiro, AU - Ito,Juichi, AU - Furuse,Mikio, AU - Tsukita,Shoichiro, PY - 2004/10/1/pubmed PY - 2005/4/26/medline PY - 2004/10/1/entrez SP - 5087 EP - 96 JF - Journal of cell science JO - J. Cell. Sci. VL - 117 IS - Pt 21 N2 - Claudins are cell adhesion molecules working at tight junctions (TJs) that are directly involved in compartmentalization in multicellular organisms. The cochlea includes a rather peculiar compartment filled with endolymph. This compartment is characterized by high K+ concentration (approximately 150 mM) and a positive endocochlear potential (approximately 90 mV; EP), both indispensable conditions for cochlear hair cells to transduce acoustic stimuli to electrical signals. These conditions are thought to be generated by the stria vascularis, which is adjacent to the endolymph compartment. The stria vascularis itself constitutes an isolated compartment delineated by two epithelial barriers, marginal and basal cell layers. Because TJs of basal cells are primarily composed of claudin-11, claudin-11-deficient (Cld11-/-) mice were generated with an expectation that the compartmentalization in stria vascularis in these mice would be affected. Auditory brainstem response measurements revealed that Cld11-/- mice suffered from deafness; although no obvious gross morphological malformations were detected in Cld11-/- cochlea, freeze-fracture replica electron microscopy showed that TJs disappeared from basal cells of the stria vascularis. In good agreement with this, tracer experiments showed that the basal cell barrier was destroyed without affecting the marginal cell barrier. Importantly, in the endolymph compartment of Cld11-/- cochlea, the K+ concentration was maintained around the normal level (approximately 150 mM), whereas the EP was suppressed down to approximately 30 mV. These findings indicated that the establishment of the stria vascularis compartment, especially the basal cell barrier, is indispensable for hearing ability through the generation/maintenance of EP but not of a high K+ concentration in the endolymph. SN - 0021-9533 UR - https://www.unboundmedicine.com/medline/citation/15456848/Compartmentalization_established_by_claudin_11_based_tight_junctions_in_stria_vascularis_is_required_for_hearing_through_generation_of_endocochlear_potential_ L2 - http://jcs.biologists.org/cgi/pmidlookup?view=long&pmid=15456848 DB - PRIME DP - Unbound Medicine ER -