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Confirmatory analysis for drugs of abuse in plasma and urine by high-performance liquid chromatography-tandem mass spectrometry with respect to criteria for compound identification.
J Chromatogr B Analyt Technol Biomed Life Sci. 2004 Nov 05; 811(1):21-30.JC

Abstract

Recently, high-performance liquid chromatography-tandem mass spectrometry (LC/MS/MS) has become a powerful tool for quantitative confirmatory analysis of drugs of abuse and has begun to spread in the field of forensic toxicology. Guidelines for confirmatory analysis by GC/MS and LC/MS/MS have been published recently by several organizations (WADA, IOC, SOFT, GTFCh, EU). However, these guidelines have not yet been included in procedures for drug analysis with LC/MS/MS. The prerequisites for forensic confirmatory analysis by LC/MS/MS with respect to EU guidelines are chromatographic separation, a minimum number of two MS/MS transitions to obtain the required identification points and predefined thresholds for the variability of the relative intensities of the MS/MS transitions (MRM transitions) in samples and reference standards. LC/MS/MS methods for determination of several classes of drugs of abuse including some basic drugs (opiates, stimulants), cannabinoids and some of their phase-I- and phase-II-metabolites (especially glucuronides) in urine and serum of drug abusers and/or crime offenders or victims have been developed and validated following current recommendations and are presented in this paper. At least two MRM transitions for each substance were monitored to provide sufficient identification of drugs, deuterated analogues of analytes were used as internal standards for quantitation where possible and chromatographic separation has been performed on reversed-phase columns with gradient elution. Validation data obtained and the application to real samples show that the requested criteria for confirmatory analysis of drugs of abuse by EU guidelines can be fulfilled with a total number of four identification points by LC/MS/MS methods using a triple-quadrupole mass spectrometer. Furthermore, the methods are sufficiently sensitive to meet current requirements for confirmatory analysis of drugs of abuse in driving under the influence of drugs (DUID) cases established by the Society of Toxicological and Forensic Chemistry (GTFCh).

Authors+Show Affiliations

Department of Analytical Chemistry, University of Pardubice, FCHT, nam. Cs. legii 565, 53210 Pardubice, Czech Republic.No affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15458717

Citation

Maralikova, Barbora, and Wolfgang Weinmann. "Confirmatory Analysis for Drugs of Abuse in Plasma and Urine By High-performance Liquid Chromatography-tandem Mass Spectrometry With Respect to Criteria for Compound Identification." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 811, no. 1, 2004, pp. 21-30.
Maralikova B, Weinmann W. Confirmatory analysis for drugs of abuse in plasma and urine by high-performance liquid chromatography-tandem mass spectrometry with respect to criteria for compound identification. J Chromatogr B Analyt Technol Biomed Life Sci. 2004;811(1):21-30.
Maralikova, B., & Weinmann, W. (2004). Confirmatory analysis for drugs of abuse in plasma and urine by high-performance liquid chromatography-tandem mass spectrometry with respect to criteria for compound identification. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 811(1), 21-30.
Maralikova B, Weinmann W. Confirmatory Analysis for Drugs of Abuse in Plasma and Urine By High-performance Liquid Chromatography-tandem Mass Spectrometry With Respect to Criteria for Compound Identification. J Chromatogr B Analyt Technol Biomed Life Sci. 2004 Nov 5;811(1):21-30. PubMed PMID: 15458717.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Confirmatory analysis for drugs of abuse in plasma and urine by high-performance liquid chromatography-tandem mass spectrometry with respect to criteria for compound identification. AU - Maralikova,Barbora, AU - Weinmann,Wolfgang, PY - 2003/12/12/received PY - 2004/04/03/accepted PY - 2004/10/2/pubmed PY - 2005/1/28/medline PY - 2004/10/2/entrez SP - 21 EP - 30 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 811 IS - 1 N2 - Recently, high-performance liquid chromatography-tandem mass spectrometry (LC/MS/MS) has become a powerful tool for quantitative confirmatory analysis of drugs of abuse and has begun to spread in the field of forensic toxicology. Guidelines for confirmatory analysis by GC/MS and LC/MS/MS have been published recently by several organizations (WADA, IOC, SOFT, GTFCh, EU). However, these guidelines have not yet been included in procedures for drug analysis with LC/MS/MS. The prerequisites for forensic confirmatory analysis by LC/MS/MS with respect to EU guidelines are chromatographic separation, a minimum number of two MS/MS transitions to obtain the required identification points and predefined thresholds for the variability of the relative intensities of the MS/MS transitions (MRM transitions) in samples and reference standards. LC/MS/MS methods for determination of several classes of drugs of abuse including some basic drugs (opiates, stimulants), cannabinoids and some of their phase-I- and phase-II-metabolites (especially glucuronides) in urine and serum of drug abusers and/or crime offenders or victims have been developed and validated following current recommendations and are presented in this paper. At least two MRM transitions for each substance were monitored to provide sufficient identification of drugs, deuterated analogues of analytes were used as internal standards for quantitation where possible and chromatographic separation has been performed on reversed-phase columns with gradient elution. Validation data obtained and the application to real samples show that the requested criteria for confirmatory analysis of drugs of abuse by EU guidelines can be fulfilled with a total number of four identification points by LC/MS/MS methods using a triple-quadrupole mass spectrometer. Furthermore, the methods are sufficiently sensitive to meet current requirements for confirmatory analysis of drugs of abuse in driving under the influence of drugs (DUID) cases established by the Society of Toxicological and Forensic Chemistry (GTFCh). SN - 1570-0232 UR - https://www.unboundmedicine.com/medline/citation/15458717/Confirmatory_analysis_for_drugs_of_abuse_in_plasma_and_urine_by_high_performance_liquid_chromatography_tandem_mass_spectrometry_with_respect_to_criteria_for_compound_identification_ DB - PRIME DP - Unbound Medicine ER -