TY - JOUR T1 - Detection of cocaine and cocaethylene in sweat by solid-phase microextraction and gas chromatography/mass spectrometry. AU - Follador,Maria José Damas, AU - Yonamine,Mauricio, AU - de Moraes Moreau,Regina Lucia, AU - Silva,Ovandir Alves, PY - 2003/11/20/received PY - 2004/03/26/accepted PY - 2004/10/2/pubmed PY - 2005/1/28/medline PY - 2004/10/2/entrez SP - 37 EP - 40 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 811 IS - 1 N2 - In the present work, a semi-quantitative method was developed to detect simultaneously cocaine (COC) and cocaethylene (CE) (transesterification product of the coingestion of COC with ethanol) in sweat. Sweat samples were collected by means of a non-occlusive sweat patch device supplied by PharmChek. The method was based on the dissolution of COC and CE incorporated into the patch, with 0.2 M sodium acetate buffer (pH 5.0) and the extraction of the analytes by solid-phase microextraction (SPME). Gas chromatography/mass spectrometry (GC-MS) was used to detect the analytes in selected ion monitoring mode (SIM). The method showed to be very simple, rapid and sensitive. The limits of detection were 5 ng/ml for COC and CE (12.5 ng/patch). Good inter and intra-assay precision was also observed (coefficient of variation <8%) with the use of deuterated internal standards. SN - 1570-0232 UR - https://www.unboundmedicine.com/medline/citation/15458719/Detection_of_cocaine_and_cocaethylene_in_sweat_by_solid_phase_microextraction_and_gas_chromatography/mass_spectrometry_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570-0232(04)00631-2 DB - PRIME DP - Unbound Medicine ER -