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Detection of cocaine and cocaethylene in sweat by solid-phase microextraction and gas chromatography/mass spectrometry.
J Chromatogr B Analyt Technol Biomed Life Sci. 2004 Nov 05; 811(1):37-40.JC

Abstract

In the present work, a semi-quantitative method was developed to detect simultaneously cocaine (COC) and cocaethylene (CE) (transesterification product of the coingestion of COC with ethanol) in sweat. Sweat samples were collected by means of a non-occlusive sweat patch device supplied by PharmChek. The method was based on the dissolution of COC and CE incorporated into the patch, with 0.2 M sodium acetate buffer (pH 5.0) and the extraction of the analytes by solid-phase microextraction (SPME). Gas chromatography/mass spectrometry (GC-MS) was used to detect the analytes in selected ion monitoring mode (SIM). The method showed to be very simple, rapid and sensitive. The limits of detection were 5 ng/ml for COC and CE (12.5 ng/patch). Good inter and intra-assay precision was also observed (coefficient of variation <8%) with the use of deuterated internal standards.

Authors+Show Affiliations

College of Pharmaceutical Sciences, Toxicology, University of S. Paulo, USP, Av. Professor Lineu Prestes, 580 B13B CEP 05508-900 São Paulo, SP, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Validation Study

Language

eng

PubMed ID

15458719

Citation

Follador, Maria José Damas, et al. "Detection of Cocaine and Cocaethylene in Sweat By Solid-phase Microextraction and Gas Chromatography/mass Spectrometry." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 811, no. 1, 2004, pp. 37-40.
Follador MJ, Yonamine M, de Moraes Moreau RL, et al. Detection of cocaine and cocaethylene in sweat by solid-phase microextraction and gas chromatography/mass spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci. 2004;811(1):37-40.
Follador, M. J., Yonamine, M., de Moraes Moreau, R. L., & Silva, O. A. (2004). Detection of cocaine and cocaethylene in sweat by solid-phase microextraction and gas chromatography/mass spectrometry. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 811(1), 37-40.
Follador MJ, et al. Detection of Cocaine and Cocaethylene in Sweat By Solid-phase Microextraction and Gas Chromatography/mass Spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci. 2004 Nov 5;811(1):37-40. PubMed PMID: 15458719.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of cocaine and cocaethylene in sweat by solid-phase microextraction and gas chromatography/mass spectrometry. AU - Follador,Maria José Damas, AU - Yonamine,Mauricio, AU - de Moraes Moreau,Regina Lucia, AU - Silva,Ovandir Alves, PY - 2003/11/20/received PY - 2004/03/26/accepted PY - 2004/10/2/pubmed PY - 2005/1/28/medline PY - 2004/10/2/entrez SP - 37 EP - 40 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 811 IS - 1 N2 - In the present work, a semi-quantitative method was developed to detect simultaneously cocaine (COC) and cocaethylene (CE) (transesterification product of the coingestion of COC with ethanol) in sweat. Sweat samples were collected by means of a non-occlusive sweat patch device supplied by PharmChek. The method was based on the dissolution of COC and CE incorporated into the patch, with 0.2 M sodium acetate buffer (pH 5.0) and the extraction of the analytes by solid-phase microextraction (SPME). Gas chromatography/mass spectrometry (GC-MS) was used to detect the analytes in selected ion monitoring mode (SIM). The method showed to be very simple, rapid and sensitive. The limits of detection were 5 ng/ml for COC and CE (12.5 ng/patch). Good inter and intra-assay precision was also observed (coefficient of variation <8%) with the use of deuterated internal standards. SN - 1570-0232 UR - https://www.unboundmedicine.com/medline/citation/15458719/Detection_of_cocaine_and_cocaethylene_in_sweat_by_solid_phase_microextraction_and_gas_chromatography/mass_spectrometry_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570-0232(04)00631-2 DB - PRIME DP - Unbound Medicine ER -