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Clinical evaluation of affirm VPIII in the detection and identification of Trichomonas vaginalis, Gardnerella vaginalis, and Candida species in vaginitis/vaginosis.
Infect Dis Obstet Gynecol. 2004; 12(1):17-21.ID

Abstract

OBJECTIVE

To compare the Affirm VPIII Microbial Identification Test for detection and identification of Candida species, Gardnerella vaginalis and Trichomonas vaginalis to clinical and microscopic criteria commonly used to diagnose vaginitis.

METHODS

Women that were symptomatic for vaginitis/vaginosis and asymptomatic women being seen for routine obstetric or gynecological care were included in this study. Women treated with antibiotics or antifungals within one week or women who had douched within 24 hours were excluded. Two vaginal swab specimens were simultaneously obtained from each patient, one swab was placed in sterile physiological saline for immediate microscopic wet mount examination and KOH testing. The other swab was placed in the Affirm collection tube for Affirm VPIII testing based on previously demonstrated methods.

RESULTS

The Affirm assay was significantly more likely to identify Gardnerella and Candida than wet mount. 190 (45%) were positive for Gardnerella by Affirm compared to 58 (14%) by wet mount; 45 (11%) were positive for Candida by Affirm compared to 31 (7%) by wet mount; and 30 (7%) were positive for Trichomonas by Affirm compared to 23 (5%) by wet mount. Symptomatic women were significantly more likely to be positive by Affirm only (23% vs. 10%), wet mount only (3% vs. 2%) or Affirm and wet mount (15% vs. 1%). Asymptomatic women were significantly more likely to be negative for Affirm and wet mount (43% vs. 5%).

CONCLUSIONS

The Affirm VPIII test is a more sensitive diagnostic test for detection and identification of symptomatic vaginitis/vaginosis than conventional clinical examination and wet mount testing.

Authors+Show Affiliations

Department of Obstetrics, Gynecology and Pathology, St. Vincent Hospitals and Health Service, Indianapolis, IN, USA. haywood.brown@duke.eduNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

15460191

Citation

Brown, Haywood L., et al. "Clinical Evaluation of Affirm VPIII in the Detection and Identification of Trichomonas Vaginalis, Gardnerella Vaginalis, and Candida Species in Vaginitis/vaginosis." Infectious Diseases in Obstetrics and Gynecology, vol. 12, no. 1, 2004, pp. 17-21.
Brown HL, Fuller DD, Jasper LT, et al. Clinical evaluation of affirm VPIII in the detection and identification of Trichomonas vaginalis, Gardnerella vaginalis, and Candida species in vaginitis/vaginosis. Infect Dis Obstet Gynecol. 2004;12(1):17-21.
Brown, H. L., Fuller, D. D., Jasper, L. T., Davis, T. E., & Wright, J. D. (2004). Clinical evaluation of affirm VPIII in the detection and identification of Trichomonas vaginalis, Gardnerella vaginalis, and Candida species in vaginitis/vaginosis. Infectious Diseases in Obstetrics and Gynecology, 12(1), 17-21.
Brown HL, et al. Clinical Evaluation of Affirm VPIII in the Detection and Identification of Trichomonas Vaginalis, Gardnerella Vaginalis, and Candida Species in Vaginitis/vaginosis. Infect Dis Obstet Gynecol. 2004;12(1):17-21. PubMed PMID: 15460191.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Clinical evaluation of affirm VPIII in the detection and identification of Trichomonas vaginalis, Gardnerella vaginalis, and Candida species in vaginitis/vaginosis. AU - Brown,Haywood L, AU - Fuller,Deanna D, AU - Jasper,Lori T, AU - Davis,Thomas E, AU - Wright,Justin D, PY - 2004/10/6/pubmed PY - 2004/10/22/medline PY - 2004/10/6/entrez SP - 17 EP - 21 JF - Infectious diseases in obstetrics and gynecology JO - Infect Dis Obstet Gynecol VL - 12 IS - 1 N2 - OBJECTIVE: To compare the Affirm VPIII Microbial Identification Test for detection and identification of Candida species, Gardnerella vaginalis and Trichomonas vaginalis to clinical and microscopic criteria commonly used to diagnose vaginitis. METHODS: Women that were symptomatic for vaginitis/vaginosis and asymptomatic women being seen for routine obstetric or gynecological care were included in this study. Women treated with antibiotics or antifungals within one week or women who had douched within 24 hours were excluded. Two vaginal swab specimens were simultaneously obtained from each patient, one swab was placed in sterile physiological saline for immediate microscopic wet mount examination and KOH testing. The other swab was placed in the Affirm collection tube for Affirm VPIII testing based on previously demonstrated methods. RESULTS: The Affirm assay was significantly more likely to identify Gardnerella and Candida than wet mount. 190 (45%) were positive for Gardnerella by Affirm compared to 58 (14%) by wet mount; 45 (11%) were positive for Candida by Affirm compared to 31 (7%) by wet mount; and 30 (7%) were positive for Trichomonas by Affirm compared to 23 (5%) by wet mount. Symptomatic women were significantly more likely to be positive by Affirm only (23% vs. 10%), wet mount only (3% vs. 2%) or Affirm and wet mount (15% vs. 1%). Asymptomatic women were significantly more likely to be negative for Affirm and wet mount (43% vs. 5%). CONCLUSIONS: The Affirm VPIII test is a more sensitive diagnostic test for detection and identification of symptomatic vaginitis/vaginosis than conventional clinical examination and wet mount testing. SN - 1064-7449 UR - https://www.unboundmedicine.com/medline/citation/15460191/Clinical_evaluation_of_affirm_VPIII_in_the_detection_and_identification_of_Trichomonas_vaginalis_Gardnerella_vaginalis_and_Candida_species_in_vaginitis/vaginosis_ DB - PRIME DP - Unbound Medicine ER -