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Ex vivo CF diagnosis by intestinal current measurements (ICM) in small aperture, circulating Ussing chambers.
J Cyst Fibros 2004; 3 Suppl 2:159-63JC

Abstract

Intestinal current measurements (ICM) on rectal suction biopsies are a tool for the ex vivo diagnosis of classical and atypical cystic fibrosis (CF). We present the basic ICM protocol, typical tracings and their interpretation. The ICM technique allows the registration of CF-induced changes in electrogenic transepithelial ion transport (Cl-, HCO3-, K+) in a Cl- secretory epithelium, and on the basis of pharmacological criteria, is able to discriminate between CFTR-mediated Cl- secretion and secretion through alternative anion channels. ICM is particularly useful for the classification of individuals with CF-like clinical features with equivocal sweat test values and/or no or one identifiable CFTR mutation.

Authors+Show Affiliations

Departments of Paediatric Gastroenterology and Biochemistry, Erasmus University Medical Centre, P.O. Box 1738, Rotterdam, 3000DR The Netherlands. h.dejonge@erasmusmc.nlNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Review

Language

eng

PubMed ID

15463951

Citation

De Jonge, Hugo R., et al. "Ex Vivo CF Diagnosis By Intestinal Current Measurements (ICM) in Small Aperture, Circulating Ussing Chambers." Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society, vol. 3 Suppl 2, 2004, pp. 159-63.
De Jonge HR, Ballmann M, Veeze H, et al. Ex vivo CF diagnosis by intestinal current measurements (ICM) in small aperture, circulating Ussing chambers. J Cyst Fibros. 2004;3 Suppl 2:159-63.
De Jonge, H. R., Ballmann, M., Veeze, H., Bronsveld, I., Stanke, F., Tümmler, B., & Sinaasappel, M. (2004). Ex vivo CF diagnosis by intestinal current measurements (ICM) in small aperture, circulating Ussing chambers. Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society, 3 Suppl 2, pp. 159-63.
De Jonge HR, et al. Ex Vivo CF Diagnosis By Intestinal Current Measurements (ICM) in Small Aperture, Circulating Ussing Chambers. J Cyst Fibros. 2004;3 Suppl 2:159-63. PubMed PMID: 15463951.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ex vivo CF diagnosis by intestinal current measurements (ICM) in small aperture, circulating Ussing chambers. AU - De Jonge,Hugo R, AU - Ballmann,Manfred, AU - Veeze,Henk, AU - Bronsveld,Inez, AU - Stanke,Frauke, AU - Tümmler,Burkhard, AU - Sinaasappel,Maarten, PY - 2004/10/7/pubmed PY - 2004/12/16/medline PY - 2004/10/7/entrez SP - 159 EP - 63 JF - Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society JO - J. Cyst. Fibros. VL - 3 Suppl 2 N2 - Intestinal current measurements (ICM) on rectal suction biopsies are a tool for the ex vivo diagnosis of classical and atypical cystic fibrosis (CF). We present the basic ICM protocol, typical tracings and their interpretation. The ICM technique allows the registration of CF-induced changes in electrogenic transepithelial ion transport (Cl-, HCO3-, K+) in a Cl- secretory epithelium, and on the basis of pharmacological criteria, is able to discriminate between CFTR-mediated Cl- secretion and secretion through alternative anion channels. ICM is particularly useful for the classification of individuals with CF-like clinical features with equivocal sweat test values and/or no or one identifiable CFTR mutation. SN - 1569-1993 UR - https://www.unboundmedicine.com/medline/citation/15463951/Ex_vivo_CF_diagnosis_by_intestinal_current_measurements__ICM__in_small_aperture_circulating_Ussing_chambers_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1569199304000888 DB - PRIME DP - Unbound Medicine ER -